Elongase of very long chain fatty acids 6 (ELOVL6) promotes lipid synthesis in buffalo mammary epithelial cells

Fan, Xinyang; Zhu, Wei; Qiu, Lihua; Zhang, Guangle; Zhang, Yongyun; Miao, Yongwang

doi:10.1111/jpn.13536

Cited by 16 publications

(12 citation statements)

References 34 publications

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“…Buffalo mammary epithelial cells (BuMECs) were obtained from a lactating buffalo (60 d postpartum; 5-year-old) and purified based on the differential sensitivity of the cells to trypsin digestion as previously described by our lab 39 , 40 . Dulbecco modified Eagle medium (Gibco, USA) supplemented with 5 μg/mL insulin (Sigma, USA), 5 μg/mL hydrocortisone (Sigma), 1 μg/mL epidermal growth factor (Sigma), 2% penicillin/streptomycin (Gibco) and 10% fetal bovine serum (Gibco) were used for the culture of purified BuMECs.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of alternative splicing variants of buffalo LXRα expressed in mammary gland

Fan

Zhang

Qiu

et al. 2022

Sci Rep

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Liver X receptor α (LXRα) is a ligand-dependent transcription factor and plays an important role in the regulation of cholesterol homeostasis, fatty acid biosynthesis and glucose metabolism. In this study, transcripts of LXRα gene were cloned and characterized from buffalo mammary gland, and three alternative splicing transcripts of buffalo LXRα gene were identified, named LXRα1, LXRα2 and LXRα3. The structure of the LXRα transcripts of buffalo and cattle was highly similar. Bioinformatics analysis showed that LXRα1 contains two complete functional domains of LXRα, one is the DNA-binding domain (NR_DBD_LXR) and the other is the ligand-binding domain (NR_LBD_LXR). The reading frame of LXRα2 is altered due to the skipping of exon 9, which truncates its encoding protein prematurely at the 400th amino acid residue, making it contain a complete DNA-binding domain and part of a ligand-binding domain. Due to the deletion of exon 4, the protein encoded by LXRα3 lacks 89 amino acid residues and contains only a complete ligand-binding domain, which makes it lose its transcriptional regulation function. In addition, motifs and conserved domains of three LXRα variants of buffalo were highly consistent with those of corresponding transcripts from other mammal species. Subcellular localization analysis showed that LXRα1 plays a functional role in the nucleus of buffalo mammary epithelial cells, while LXRα2 and LXRα3 are distributed in the nucleus and cytoplasm. Compared with non-lactating period, the mRNA abundance of the three LXRα transcripts in the mammary gland tissue of buffalo increased during lactating period, revealing that they play a key role in the synthesis of buffalo milk fat. Among the three LXRα transcripts, LXRα1 has the highest expression in the mammary gland, indicating that it is the major transcript in the mammary gland and has important regulatory functions, while LXRα2 and LXRα3 may have regulatory effects on the function of LXRα1. This study highlights the key role of LXRα alternative splicing in the post-transcriptional regulation of buffalo lactation.

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Section: Methodsmentioning

confidence: 99%

Identification and characterization of alternative splicing variants of buffalo LXRα expressed in mammary gland

Fan

Zhang

Qiu

et al. 2022

Sci Rep

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“…In a recent study [ 111 ] on BuMECs, it was revealed that the enzyme elongase (responsible for long-chain fatty acid synthesis) promotes lipid synthesis during lactation by synthesizing long-chain fatty acids and also regulating the expression levels of other related genes for milk fat synthesis during lactation. A comparative proteome study of exosome-derived proteins (exosomes isolated from cultured bovine mammary epithelial cells (BMECs) and milk) revealed the involvement of exosome proteins in signaling pathways regulating the lactation process [ 112 ].…”

Section: Structural and Functional Development Of The Bovine Mammary ...mentioning

confidence: 99%

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Jaswal

Jena

Anand

et al. 2022

Cells

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The mammary gland is a unique organ with the ability to undergo repeated cyclic changes throughout the life of mammals. Among domesticated livestock species, ruminants (cattle and buffalo) constitute a distinct class of livestock species that are known milk producers. Cattle and buffalo contribute to 51 and 13% of the total milk supply in the world, respectively. They also play an essential role in the development of the economy for farming communities by providing milk, meat, and draft power. The development of the ruminant mammary gland is highly dynamic and multiphase in nature. There are six developmental stages: embryonic, prepubertal, pubertal, pregnancy, lactation, and involution. There has been substantial advancement in our understanding of the development of the mammary gland in both mouse and human models. Until now, there has not been a thorough investigation into the molecular processes that underlie the various stages of cow udder development. The current review sheds light on the morphological and molecular changes that occur during various developmental phases in diverse species, with a particular focus on the cow udder. It aims to explain the physiological differences between cattle and non-ruminant mammalian species such as humans, mice, and monkeys. Understanding the developmental biology of the mammary gland in molecular detail, as well as species-specific variations, will facilitate the researchers working in this area in further studies on cellular proliferation, differentiation, apoptosis, organogenesis, and carcinogenesis. Additionally, in-depth knowledge of the mammary gland will promote its use as a model organ for research work and promote enhanced milk yield in livestock animals without affecting their health and welfare.

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“…Overexpression or interference ELOVL6 can promote the expression of FASN in BMECs and also has positive regulatory effects on insulin-induced genes (INSIG1 and INSIG2), SREBP1 and PPARG. So, we hypothesized that ELOVL6 could rely on transcription factors INSIG, SREBP1 and PPARG to regulate FASN ( 51 ). cAMP responsive element binding protein 1 (CREB1) is a member of the leucine zipper transcription factor family of DNA binding proteins, which is involved in the regulation of cellular lipid metabolism ( 52 ).…”

Section: Positive Regulation Of De Novo Synthesis Of Famentioning

confidence: 99%

“…In addition, ELOVL6, EGFR and THRSP also have positively regulated SCD1. The regulation of SCD1 by EGFR depends on the AKT- and PLC-γ-1 signaling pathways ( 38 , 51 , 56 ).…”

Section: Lcfa Transport and Desaturationmentioning

confidence: 99%

“…It is suggest that AKT1 plays an important role in regulating GMECs TAG synthesis, which may be through the mTOR/SREBP1 axis. Shi et al ( 112 ) showed that inhibition ELOVL6 in GMECs significantly reduced GPAM expression and TAG concentration, ELOVL6 similarly positively regulated GPAM and AGPAT6 expression in buffalo MECs, suggesting that ELOVL6 not only regulates fatty acid chain extension and is involved in the de novo synthesis of FA, but also has a positive regulatory role in TAG synthesis ( 51 ). In addition, the GPAM is also positively regulated by THRSP in GMECs ( 38 ).…”

Section: Tag Synthesismentioning

confidence: 99%

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Regulation of Key Genes for Milk Fat Synthesis in Ruminants

et al. 2021

Front. Nutr.

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Milk fat is the most important and energy-rich substance in milk and plays an important role in the metabolism of nutrients during human growth and development. It is mainly used in the production of butter and yogurt. Milk fat not only affects the flavor and nutritional value of milk, but also is the main target trait of ruminant breeding. There are many key genes involve in ruminant milk fat synthesis, including ACSS2, FASN, ACACA, CD36, ACSL, SLC27A, FABP3, SCD, GPAM, AGPAT, LPIN, DGAT1, PLIN2, XDH, and BTN1A1. Taking the de novo synthesis of fatty acids (FA) and intaking of long-chain fatty acids (LCFA) in blood to the end of lipid droplet secretion as the mainline, this manuscript elucidates the complex regulation model of key genes in mammary epithelial cells (MECs) in ruminant milk fat synthesis, and constructs the whole regulatory network of milk fat synthesis, to provide valuable theoretical basis and research ideas for the study of milk fat regulation mechanism of ruminants.

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Elongase of very long chain fatty acids 6 (ELOVL6) promotes lipid synthesis in buffalo mammary epithelial cells

Cited by 16 publications

References 34 publications

Identification and characterization of alternative splicing variants of buffalo LXRα expressed in mammary gland

Identification and characterization of alternative splicing variants of buffalo LXRα expressed in mammary gland

Critical Review on Physiological and Molecular Features during Bovine Mammary Gland Development: Recent Advances

Regulation of Key Genes for Milk Fat Synthesis in Ruminants

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