Regulation of Key Genes for Milk Fat Synthesis in Ruminants

Mu, Tong; Hu, Honghong; Ma, Yanfen; Feng, Xiaoran; Zhang, Juan; Gu, Ye

doi:10.3389/fnut.2021.765147

Cited by 49 publications

(35 citation statements)

References 126 publications

(204 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased intramuscular fat accumulation in goats fed ABSF is most likely due to the increased rumen acetate production. In fact, an increase in acetate production makes more substrate accessible for de novo fat synthesis ( 41 ). Previous research found a negative relationship between intramuscular fat content and WBSF tenderness values ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

et al. 2022

View full text Add to dashboard Cite

Pre-treating anthocyanin-rich black cane with ferrous sulfate heptahydrate (FSH) produces high-quality silage with anthocyanin and nutritional losses. However, it's unclear how to apply this to studies on how FSH-treated silage affects animal performance and meat quality. Therefore, this study aimed to investigate the effects of a standard total mixed ration (TMR) containing anthocyanin-rich black cane silages either with or without dilutions of FSH on animal performance, blood biochemical indices, rumen fermentation, microbial community, and carcass characteristics in meat goats. Forty healthy crossbred Thai-native Anglo-Nubian male goats (14.42 ± 1.4 kg) were used to compare the feasibility of using anthocyanin-rich black cane silage (ABS) as a functional feed resource as opposed to anthocyanin-rich black cane treated with 0.030% commercial FSH silage (ABSF). All goats received a 90-day routine feeding of two isocaloric and isonitrogenous experimental diets: the control group received TMR containing 50% anthocyanin-rich black cane silage (ABS; n = 20), and one group received TMR containing 50% FSH-treating anthocyanin-rich black cane (ABSF; n = 20). As performance indicators, average daily weight gain (ADG) and dry matter intake (DMI) were measured. Samples of meat, blood, and rumen were taken at the end of the experiment. There were no differences in final body weight, ADG, DMI, or ADG/DMI between the two groups. The ABSF group did not differ from the ABS group in terms of rumen pH, but the ABSF had a tendency to lower rumen ammonia N levels, and to higher total volatile fatty acid (VFA) concentrations. Individual VFA concentrations differed, with the ABSF group having more Ruminococcus albus and the ABS group having more methanogenic bacteria. Blood biochemical indices differed, with the ABSF group having lower TBARS concentrations and the ABS group having lower TAC, SOD, CAT, GSH-Px, and GSH-Rx concentrations. In comparison to goat meat from the ABS group, goat meat from the ABSF group contained more intramuscular fat and was more tender. The current results indicate that the feeding of a TMR containing 50% anthocyanin-rich black cane, along with FSH pre-treatment prior to ensiling, reduces oxidative stress and promotes the production of tender meat without affecting animal performance.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In our dataset, multiple proteins and phosphoproteins with functions in lipid metabolism were altered due to heat stress. At 14 DIM, perilipin (PLIN), a lipid droplet associated protein that participates in lipid droplet secretion from mammary epithelial cells 44 and acyl-CoA synthetase long chain family member 1 (ACSL), with a putative role in long chain fatty acid uptake by mammary cells 45 , were more abundant in HT cows. On the contrary, acetyl CoA carboxylase (ACACA), a rate limiting enzyme in milk fatty acid synthesis, was decreased in HT cows at 84 DIM.…”

Section: Discussionmentioning

confidence: 99%

Carry-over effects of dry period heat stress on the mammary gland proteome and phosphoproteome in the subsequent lactation of dairy cows

Skibiel

Koh

Zhu

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Exposure to heat stress during a cow’s dry period disrupts mammary gland remodeling, impairing mammary function and milk production during the subsequent lactation. Yet, proteomic changes in the mammary gland underlying these effects are not yet known. We investigated alterations in the mammary proteome and phosphoproteome during lactation as a result of dry period heat stress using an isobaric tag for relative and absolute quantitation (iTRAQ)-based approach. Cows were cooled (CL; n = 12) with fans and water soakers in a free stall setting or were heat stressed through lack of access to cooling devices (HT; n = 12) during the entire dry period (approximately 46 days). All cows were cooled postpartum. Mammary biopsies were harvested from a subset of cows (n = 4 per treatment) at 14, 42, and 84 days in milk. Overall, 251 proteins and 224 phosphorylated proteins were differentially abundant in the lactating mammary gland of HT compared to CL cows. Top functions of differentially abundant proteins and phosphoproteins affected were related to immune function and inflammation, amino acid metabolism, reactive oxygen species production and metabolism, tissue remodeling, and cell stress response. Patterns of protein expression and phosphorylation are indicative of increased oxidative stress, mammary gland restructuring, and immune dysregulation due to prior exposure to dry period heat stress. This study provides insights into the molecular underpinnings of disrupted mammary function and health during lactation arising from prior exposure to dry period heat stress, which might have led to lower milk yields.

show abstract

“…Milk quality is affected by many factors, such as population genetic structure, reproductive performance, feeding and management, and is closely related to the main raw material composition of milk. Milk fat is an important component in butter and yogurt, and its content and composition are the main reference elements in milk quality evaluation ( Mu et al, 2021 ). Nowadays, milk fat content is not only one of the important indicators of core competitiveness of dairy industry, but also the main target feature of dairy cow breeding ( Li et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Screening and Conjoint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows

Feng

et al. 2022

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) play an important regulatory role in various biological processes as a key regulatory factor. However, the complete expression profile of lncRNAs in dairy cows and its function in milk fat synthesis are unknown. In this study, RNA sequencing (RNA-seq) was used to research the whole genome expression of lncRNAs and mRNA transcripts in high and low milk fat percentage (MFP) bovine mammary epithelial cells (BMECs), and joint analysis was carried out. We identified a total of 47 differentially expressed genes (DEGs) and 38 differentially expressed lncRNAs (DELs, Padj <0.05), enrichment analysis screened out 11 candidate DEGs that may regulate milk fat metabolism. Downregulated differential gene ENPP2 (The expression level in BMECs of high milk fat dairy cows was lower than that of low milk fat cows) and upregulated differential gene BCAT1 are more likely to participate in the milk fat metabolism, and its function needs further experiments verification. The enrichment analysis of target genes predicted by DELs identified 7 cis (co-localization) and 10 trans (co-expression) candidate target genes related to milk lipid metabolism, corresponding to a total of 18 DELs. Among them, the targeting relationship between long intervening/intergenic noncoding RNA (lincRNA) TCONS_00082721 and FABP4 is worthy of attention. One hundred and fifty-six competing endogenous RNAs (ceRNAs) interaction regulation networks related to milk fat metabolism were constructed based on the expression information of DELs, differential microRNAs (miRNAs), and lipid metabolism-related target genes. The regulatory network centered on miR-145 will be the focus of subsequent experimental research. The ceRNAs regulatory network related to TCONS_00082721 and TCONS_00172817 are more likely to be involved in milk fat synthesis. These results will provide new ways to understand the complex biology of dairy cow milk fat synthesis and provide valuable information for breed improvement of Chinese Holstein cow.

show abstract

Regulation of Key Genes for Milk Fat Synthesis in Ruminants

Cited by 49 publications

References 126 publications

Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

Dietary inclusion of anthocyanin-rich black cane silage treated with ferrous sulfate heptahydrate reduces oxidative stress and promotes tender meat production in goats

Carry-over effects of dry period heat stress on the mammary gland proteome and phosphoproteome in the subsequent lactation of dairy cows

Screening and Conjoint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows

Contact Info

Product

Resources

About