Elliptical optical tweezers for trapping a red blood cell aggregate

Kauppila, Antti; Karmenyan, Artashes; Myllylä, Risto

doi:10.4302/plp.2011.3.02

Cited by 1 publication

(1 citation statement)

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“…Also other biomolecules, such as dextran or glucose, increase background scattering, which distorts the measurements. 49 The specificity of this experiment results from combining optimal conditions for two phenomena, determined by the RI difference between a sample and its environment. To facilitate optical clearing, it is necessary (desirable) to increase the refraction index of the cell's environment in order to reduce light scattering by the cell.…”

Section: Resultsmentioning

confidence: 99%

Optical clearing at cellular level

et al. 2014

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Strong light scattering in tissues and blood reduces the usability of many optical techniques. By reducing scattering, optical clearing enables deeper light penetration and improves resolution in several optical imaging applications. We demonstrate the usage of optical tweezers and elastic light scattering to study optical clearing [one of the major mechanisms-matching of refractive indices (RIs)] at the single particle and cell level. We used polystyrene spheres and human red blood cells (RBCs) as samples and glycerol or glucose water solutions as clearing agents. Optical tweezers kept single microspheres and RBCs in place during the measurement of light scattering patterns. The results show that optical clearing reduces the scattering cross section and increases g. Glucose also decreased light scattering from a RBC. Optical clearing affected the anisotropy factor g of 23.25-μm polystyrene spheres, increasing it by 0.5% for an RI change of 2.2% (20% glycerol) and 0.3% for an RI change of 1.1% (13% glucose).

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Section: Resultsmentioning

confidence: 99%