Ellagic acid prevents kidney injury and oxidative damage via regulation of Nrf-2/NF-κB signaling in carbon tetrachloride induced rats

Aslan, Abdullah Nabi; Gök, Özlem; Beyaz, Seda; Ağca, Can Ali; Erman, Orhan; Zerek, Aykut

doi:10.1007/s11033-020-05873-x

Cited by 28 publications

(11 citation statements)

References 52 publications

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“…But high-glucose treatment significantly decreased the expression of Nrf2 in a time-dependent manner [ 4 ]. Previous studies have showed that ellagic acid reduced oxidative stress and insulin resistance in diabetic female rats by increasing the Nrf2 protein expression [ 25 , 49 ]. In the present study, even at low concentrations (1 and 10 µg/mL), the camu-camu fruit extract exhibited better preventative effects against high glucose-induced ROS generation by regulating the Nrf2 pathway than that of dexamethasone and cyclosporin A ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

et al. 2021

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Myrciaria dubia (HBK) McVaugh (camu-camu) belongs to the family Myrtaceae. Although camu-camu has received a great deal of attention for its potential pharmacological activities, there is little information on the anti-oxidative stress and anti-inflammatory effects of camu-camu fruit in skin diseases. In the present study, we investigated the preventative effect of 70% ethanol camu-camu fruit extract against high glucose-induced human keratinocytes. High glucose-induced overproduction of reactive oxygen species (ROS) was inhibited by camu-camu fruit treatment. In response to ROS reduction, camu-camu fruit modulated the mitogen-activated protein kinases (MAPK)/activator protein-1 (AP-1), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and nuclear factor of activated T cells (NFAT) signaling pathways related to inflammation by downregulating the expression of proinflammatory cytokines and chemokines. Furthermore, camu-camu fruit treatment activated the expression of nuclear factor E2-related factor 2 (Nrf2) and subsequently increased the NAD(P)H:quinone oxidoreductase1 (NQO1) expression to protect keratinocytes against high-glucose-induced oxidative stress. These results indicate that camu-camu fruit is a promising material for preventing oxidative stress and skin inflammation induced by high glucose level.

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Section: Discussionmentioning

confidence: 99%

Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

et al. 2021

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“…Depending on the working range, starting from 30 μl, gradually increasing concentrations of supernatant were added and mixed once. In the spectrophotometer, the absorbance change of H 2 O 2 was read at 240-nm wavelength for 30 s. The number of enzyme units per milliliter was calculated from this optical density difference (Luck 1963 ; Yigitcan 2012 ; Aslan et al 2020a ).…”

Section: Methodsmentioning

confidence: 99%

“…It is used to improve human health due to its remarkable antimicrobial and antioxidant properties. It is made up of various bioactive substances such as water (50–60%), proteins (18%), carbohydrates (15%), lipids (3–6%), vitamins, free amino acids and mineral salts (1.5%) (Aslan 2015 ; Ozsahin et al 2019 ; Sharif and Darsareh 2019 ; Park et al 2020 ; Aslan et al 2020a ; Demir and Aslan 2020 ). Royal jelly contains many active constituents and antioxidants such as polyphenols (Hossen et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The inducing of caspase and Bcl-2 pathway with royal jelly decreases the muscle tissue damage exposed with fluoride in rats

Aslan

Can

Gök

et al. 2021

Environ Sci Pollut Res

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Graphical abstract In this study, 42 Wistar albino male rats ( n = 42, 8 weeks old) were used. Rats were divided into 6 groups and 7 rats included each group. Groups: (i) Control group: Standard diet; (ii) RJ (royal jelly) group: Standard diet + royal jelly; (iii) F50 group: Standard diet + 50 mg/kg fluoride; (iv): F100 group: Standard diet + 100 mg/kg fluoride; (v) F50+RJ group: Standard diet + 50 mg/kg fluoride + royal jelly; (vi): F100+RJ group: Standard diet + 100 mg/kg fluoride + royal jelly. After 8 weeks, the rats were decapitated, and their muscle tissues were removed. Expression levels of Caspase-3, Caspase-6, Bax, tumor necrosis factor-α (TNF-α), interleukin 1 alpha (IL1-α) and Bcl-2 proteins in muscle tissue were determined by western blotting method. Histopathological analyses were also performed on the muscle tissue. Malondialdehyde (MDA), glutathione (GSH) and catalase (CAT) analyses were determined by a spectrophotometer. According to the obtained results, Bcl-2, TNF-α and IL1-α protein expression was increased in damage groups compared to the control and royal jelly groups, while Caspase-3, Caspase-6 and Bax protein expression levels decreased in damage groups. MDA level increased in damage groups compared to the control and royal jelly groups, while CAT and GSH levels increased with royal jelly application in royal jelly-given group in comparison to the flouride-exposed group. According to histopathological analysis results, edema and inflammatory cell formations were found in the injury groups, a tendency to decrease in these injuries was observed in the treatment groups. Based on these results, we can say that royal jelly has protective effects on muscle tissue against fluoride damage.

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“…In the spectrophotometer, the absorbance change of H 2 O 2 is read at 240 nm wavelength for 30 seconds. The number of enzyme units per milliliter is calculated from this optical density difference (Luck 1963;Yigitcan 2012;Aslan et al 2020a).…”

Section: Catalase (Cat) Activity Measurement In Muscle Tissuementioning

confidence: 99%

“…It is used to improve human health due to its remarkable antimicrobial and antioxidant properties. It is made up of various bioactive substances such as water (50-60%), proteins (18%), carbohydrates (15%), lipids (3-6%), vitamins, free amino acids and mineral salts (1,5%) (Aslan 2015;Ozsahin et al 2019;Sharif and Darsareh 2019;Park et al 2020;Aslan et al 2020a;Demir et al 2020). Royal jelly is known to have antitumor, antioxidative, antidiabetic, antiallergic, antibacterial, antiin ammatory and antihypertensive effects.…”

Section: Introductionmentioning

confidence: 99%

The inducing of caspase and bcl-2 pathway with royal jelly decreases the muscle tissue damage exposed with fluoride in rats

Aslan

Can

Gök

et al. 2021

Preprint

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In this study, 42 Wistar albino female rats (n = 42, 8 weeks old) were used. Rats were divided into 6 groups and 7 rats included each group. Groups: (i) Control Group: Standard diet; (ii) RJ (royal jelly) Group: Standard diet + royal jelly; (iii) F50 Group: Standard diet + 50 mg/kg fluoride; (iv): F100 Group: Standard diet + 100 mg/kg fluoride; (v) F50 + RJ Group: Standard diet + 50 mg/kg fluoride + royal jelly; (iv): F100 + RJ Group: Standard diet + 100 mg/kg fluoride + royal jelly. After the 8-week study period, the rats were decapitated and their muscle tissues were removed. Expression levels of Caspase-3, Caspase-6, Bax, Tnf-α, IL1-α and Bcl-2 proteins in muscle tissue were determined by Western Blotting method. Histopathological analyzes were also performed on the muscle tissue. MDA, GSH, and CAT analyzes were determined by spectrophotometric analysis. According to our findings, Bcl-2, Tnf-α and IL1-α protein expression were increased in damage groups compared to control and royal jelly groups, Caspase-3, Caspase-6 and Bax protein expression levels decreased in damage groups. There was an increase in MDA level in damage groups compared to the control and royal jelly groups, CAT and GSH levels decreased in damage groups. According to histopathological analysis results, edema and inflammatory cell formations were found in the injury groups, a tendency to decrease in these injuries was observed in the treatment groups. Based on these results, we can say that royal jelly has protective effects against fluoride damage.

show abstract

Ellagic acid prevents kidney injury and oxidative damage via regulation of Nrf-2/NF-κB signaling in carbon tetrachloride induced rats

Cited by 28 publications

References 52 publications

Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

The inducing of caspase and Bcl-2 pathway with royal jelly decreases the muscle tissue damage exposed with fluoride in rats

The inducing of caspase and bcl-2 pathway with royal jelly decreases the muscle tissue damage exposed with fluoride in rats

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