Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

Quynh, Nhung; Zheng, Shengdao; Park, Bom; Nguyen, Quynh T. N.; Choi, Bo Ram; Fang, Minzhe; Kim, Min‐Seon; Jeong, Jeehaeng; Choi, Jong-In; Yang, Su Jin; Yi, Tae–Hoo

doi:10.3390/molecules26113174

Cited by 14 publications

(18 citation statements)

References 48 publications

(40 reference statements)

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“…The Nrf2 signaling pathway plays a key role in the antioxidant defense system against oxidative stress. 42 Expression of HO-1 is regulated by Nrf2. Oxidative stress encourages translocation of Nrf2 to the nucleus, where it upregulates the HO-1.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of Catalpol Intranasal Administration and Its Protective Effect on Acute Cerebral Ischemia in Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Wang¹,

Zhang²,

Zhang³

et al. 2022

DDDT

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Purpose Catalpol is the main active component of Rehmannia glutinosa, which has a variety of pharmacological activities, including anti-inflammatory and anti-oxidative effects. This study investigates the feasibility of catalpol intranasal administration and its protective effect on acute cerebral ischemia in rats via anti-oxidative and anti-apoptotic mechanisms. Patients and Methods This study investigates the method of catalpol intranasal administration to evaluate the nasal mucosal toxicity, brain targeting and pharmacokinetics of catalpol. The protective effect of catalpol of intranasal administration on stroke-induced brain injury in rats and its mechanisms on oxidative stress pathway Nrf2/HO-1 and apoptosis were also investigated using middle cerebral artery occlusion (MCAO). Results The results showed that catalpol intranasal administration was safe and feasible with no hemolysis, no bad effect on the maxillary ciliary movement of bullfrog. After intranasal administration, the brain targeting index (DTI) of catalpol was greater than 1, which indicated that catalpol had good brain targeting after intranasal administration. The bioavailability of catalpol administered intranasally was higher than that of in plasma. In MACO model, catalpol intranasal administration could significantly reduce cerebral infarction volume, neurological dysfunction and brain edema. In addition, catalpol intranasal administration can also reduce the brain cell’s occurrence of apoptosis, promote the expression of Bcl-2 protein and inhibit the expression of Bax protein, reduce oxidative stress damage via up-regulating expression of Nrf2 and HO-1, increasing the activities of SOD and decreasing the activities of MDA. Conclusion Collectively, catalpol intranasal administration has good safety, stability and brain targeting. It can effectively protect the brain injury of the rat model of acute cerebral ischemia and provide the possibility of drug administration in the acute stage of cerebral ischemia, especially before entering the hospital.

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Section: Discussionmentioning

confidence: 99%

Feasibility of Catalpol Intranasal Administration and Its Protective Effect on Acute Cerebral Ischemia in Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Wang¹,

Zhang²,

Zhang³

et al. 2022

DDDT

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Section: Effect Of Ljp On Nrf2 Ho-1 and Nqo1 Protein Expression In Sgs After Irradiationmentioning

confidence: 99%

“…It is well known that Nrf2 signaling pathway is the most important pathway against oxidative stress [32]. Hence, we investigated the effect of LJP on Nrf2 and its downstream antioxidases, heme oxygenase-1 (HO-1) and quinone oxidoreductase-1 (NQO1), post radiation using immuno uorescence or immunohistochemistry.…”

Section: Effect Of Ljp On Nrf2 Ho-1 and Nqo1 Protein Expression In Sgs After Irradiationmentioning

confidence: 99%

Polysaccharide From Laminaria Japonica Attenuates Radiation-Induced Xerostomia In Mice Via Activation of Nrf2 Signaling Pathway

Zhang

Chen

et al. 2021

Preprint

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Background：Xerostomia is one of the most common complications during radiotherapy for head and neck cancer patients that seriously affects their quality of life. However, optimal treatment for radiation-induced xerostomia is currently unavailable. Therefore, this study aimed to investigate the protective effects of Laminaria Japonica Polysaccharide (LJP) on radiation-induced xerostomia and decipher its underlying mechanism in mice.Methods：Male eight-week-old mice were randomly divided into four groups：normal control group, LJP control group, irradiation group, and irradiation with LJP treatment group. LJP (40mg/kg) was intraperitoneally injected at 1 day before irradiation once daily for consecutive 5 days．The mice irradiated received a single radiation dose of 15Gy. Body weight, daily food and water intake were measured at 28 days post irradiation. Oxidative stress parameters (ROS and MDA) and antioxidant enzyme (MnSOD) in submandibular glands (SMG) were also assessed post irradiation. In addition, histopathology, function and weight of SMG were detected after irradiation. The expressions of Nrf2 pathway-related genes (Nrf2, HO-1 and NQO1) were measured using immunofluorescence or immunohistochemical staining and real-time PCR method. Results：Compared with the control group, irradiated mice showed increases in the levels of oxidative stress parameters (ROS and MDA), water intake and expression levels of Nrf2 pathway-related genes (Nrf2, HO-1, and NQO1), and decreases in body weight, food intake, saliva flow. Nevertheless, LJP treatment significantly increased body weights, food intake, and saliva flow in irradiated mice, markedly downregulated oxidative stress levels, improved the morphology and structure of SMG, and further promote expressions of Nrf2 pathway-related genes.Conclusion: LJP treatment alleviated radiation-induced SMG oxidative stress injury through activating Nrf2 signaling pathway, implying that LJP might be a novel agent for treating radioactive xerostomia in the future.

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“…Along with anti-oxidant activities, camu-camu has shown pharmacological effects, including those that are anti-inflammatory, anti-microbial, anti-hypertensive, and anti-obesity [ 15 , 16 , 17 , 18 ]. In a previous study, we reported the anti-inflammatory activities of camu-camu fruit extract by modulating MAPK and NFAT signaling pathways in keratinocytes [ 19 ]. Despite exerting various pharmacological effects, the anti-allergic effect of camu-camu fruit has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Anti-Allergic Effects of Myrciaria dubia (Camu-Camu) Fruit Extract by Inhibiting Histamine H1 and H4 Receptors and Histidine Decarboxylase in RBL-2H3 Cells

Quynh

Zheng

Oh³

et al. 2021

Antioxidants

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Although Myrciaria dubia (camu-camu) has been shown to exert anti-oxidant and anti-inflammatory effects in both in vitro and in vivo studies, its use in allergic responses has not been elucidated. In the present study, the anti-allergic effect of 70% ethanol camu-camu fruit extract was tested on calcium ionophore (A23187)-induced allergies in RBL-2H3 cells. The RBL-2H3 cells were induced with 100 nM A23187 for 6 h, followed by a 1 h camu-camu fruit extract treatment. A23187 sanitization exacerbated mast cell degranulation; however, camu-camu fruit extract decreased the release of histamine and β-hexosaminidase, which are considered as key biomarkers in cell degranulation. Camu-camu fruit extract inhibited cell exocytosis by regulating the calcium/nuclear factor of activated T cell (NFAT) signaling. By downregulating the activation of mitogen-activated protein kinase (MAPK) signaling, camu-camu fruit extract hindered the activation of both histamine H1 and H4 receptors and inhibited histidine decarboxylase (HDC) expression by mediating its transcription factors KLF4/SP1 and GATA2/MITF. In A23187-induced ROS overproduction, camu-camu fruit extract activated nuclear factor erythroid-2-related factor 2 (Nrf2) to protect mast cells against A23187-induced oxidative stress. These findings indicate that camu-camu fruit extract can be developed to act as a mast cell stabilizer and an anti-histamine. This work also “opens the door” to new investigations using natural products to achieve breakthroughs in allergic disorder treatment.

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Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

Cited by 14 publications

References 48 publications

Feasibility of Catalpol Intranasal Administration and Its Protective Effect on Acute Cerebral Ischemia in Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Feasibility of Catalpol Intranasal Administration and Its Protective Effect on Acute Cerebral Ischemia in Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Polysaccharide From Laminaria Japonica Attenuates Radiation-Induced Xerostomia In Mice Via Activation of Nrf2 Signaling Pathway

Anti-Allergic Effects of Myrciaria dubia (Camu-Camu) Fruit Extract by Inhibiting Histamine H1 and H4 Receptors and Histidine Decarboxylase in RBL-2H3 Cells

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