Ellagic acid enhances antitumor efficacy of temozolomide in an in vitro glioblastoma model

Çetin, Abdurrahman; Biltekin, Burcu

doi:10.5137/1019-5149.jtn.26026-19.1

Cited by 9 publications

(9 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C6 glioma cells were purchased from the American Type Culture Collection (Manassas, VA, USA), and all procedures were in accordance with steps described in previous studies 13 , 14 .…”

Section: Methodsmentioning

confidence: 99%

“…To determine the immunoreactivities of E-cadherin, N-cadherin, and VEGF, all steps of immunocytochemistry (ICC) and H-SCORE analysis described in previous studies were applied in this study 13 , 14 .…”

Section: Methodsmentioning

confidence: 99%

“…The expression levels of E-cadherin, N-cadherin, and VEGF were determined following all steps of real-time quantitative polymerase chain reaction (qPCR) described in our previous studies 13 , 14 .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Antitumor activity of irinotecan with ellagic acid in C6 glioma cells

Çetin

Biltekin

Özevren

2022

Rev. Assoc. Med. Bras.

Self Cite

View full text Add to dashboard Cite

OBJECTIVE:Irinotecan-based combination chemotherapies in malignant gliomas need to be examined. The aim of this study was to investigate the synergetic effect of ellagic acid, a natural polyphenolic antioxidant compound, with irinotecan, an inhibitor of topoisomerase I enzyme, on the growth, cadherin switch, and angiogenic processes of a glioma cell line. METHODS: A combination of 100 μM ellagic acid and 100 μM irinotecan was applied to rat C6 glioma cells for 24th, 48th, and 72nd h. The cell proliferation was evaluated by 5-bromo-2ʹ-deoxyuridine immunocytochemistry. The expression levels of vascular endothelial growth factor, E-cadherin, and N-cadherin were measured using real-time polymerase chain reaction and their immunoreactivities using immunocytochemistry. RESULTS: The treatment of irinotecan with combining ellagic acid enhanced antitumor activity and the synergistic effect of these reduced the cell proliferation of C6 glioma by inhibiting the cadherin switch and promoting the antiangiogenic processes. CONCLUSIONS: Further research is required to prove a negative relationship between C6 glial cell proliferation and irinotecan with ellagic acid application. Our preliminary data suggest that even with the extremely short-term application, irinotecan with ellagic acid may affect glioma cells at the level of gene and protein expression.

show abstract

“…C6 glioma cells were purchased from the American Type Culture Collection (Manassas, VA, USA), and all procedures were in accordance with steps described in previous studies 13 , 14 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Antitumor activity of irinotecan with ellagic acid in C6 glioma cells

Çetin

Biltekin

Özevren

2022

Rev. Assoc. Med. Bras.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This encapsulation improved the oral bioavailability and the anti-tumor effect of ellagic acid. In a glioblastoma model, Cetin, et al carried out two studies that showed an improvement in the anticancer efficacy of bevacizumab [ 64 ] and TMZ [ 65 ] by co-treatment with ellagic acid. This treatment reduced the expression of MGMT, affected caspase-3 and p53 proteins, and its combination with the chemotherapeutics reduced cell viability and the expression of MDR1.…”

Section: Dietary Phenolic Compounds Improving the Chemosensitivity Of...mentioning

confidence: 99%

Advances in Dietary Phenolic Compounds to Improve Chemosensitivity of Anticancer Drugs

Bouyahya

Omari

Bakrim

et al. 2022

Cancers

View full text Add to dashboard Cite

Despite the significant advances and mechanistic understanding of tumor processes, therapeutic agents against different types of cancer still have a high rate of recurrence associated with the development of resistance by tumor cells. This chemoresistance involves several mechanisms, including the programming of glucose metabolism, mitochondrial damage, and lysosome dysfunction. However, combining several anticancer agents can decrease resistance and increase therapeutic efficacy. Furthermore, this treatment can improve the effectiveness of chemotherapy. This work focuses on the recent advances in using natural bioactive molecules derived from phenolic compounds isolated from medicinal plants to sensitize cancer cells towards chemotherapeutic agents and their application in combination with conventional anticancer drugs. Dietary phenolic compounds such as resveratrol, gallic acid, caffeic acid, rosmarinic acid, sinapic acid, and curcumin exhibit remarkable anticancer activities through sub-cellular, cellular, and molecular mechanisms. These compounds have recently revealed their capacity to increase the sensitivity of different human cancers to the used chemotherapeutic drugs. Moreover, they can increase the effectiveness and improve the therapeutic index of some used chemotherapeutic agents. The involved mechanisms are complex and stochastic, and involve different signaling pathways in cancer checkpoints, including reactive oxygen species signaling pathways in mitochondria, autophagy-related pathways, proteasome oncogene degradation, and epigenetic perturbations.

show abstract

“…The immunocytochemical analysis of tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and matrix metalloproteinase-2 (MMP-2) and MMP-9 was performed using a horseradish peroxidase/AEC Detection IHC Kit (ScyTek Laboratories, USA, cat no: ACD002) according to the literature 11 . Primary antibodies of TNF-α (Santa Cruz Biotechnology Cas: 52B83, Lot: G2018), TGF-β (ThermoFisher Scientific, MA, USA, product no: MA5-16949), MMP-2 antibody (Invitrogen, USA, MA5-13590), and MMP-9 antibody (Invitrogen, USA, MA5-15886) were diluted at 1/100, and 150 μl of diluted antibodies were added onto 1×10 4 cells.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line

Karagülle¹,

Yurttaş

2022

Rev. Assoc. Med. Bras.

View full text Add to dashboard Cite

OBJECTIVE:We aimed to examine the potential anticancer effects of ozone applied after chemotherapeutic treatment with different concentrations of doxorubicin in Luminal-A subtype of human breast cancer cell line (MCF-7) and compare the results with effects on L929 fibroblast cell line. METHODS: Both cell lines were incubated with increasing doses of doxorubicin (1-50 μM) for 24 h at 37 o C. Then, half of groups were incubated with 30 μg/mL ozone for 25 min as combination groups. Cell viability was analyzed by MTT assay, apoptosis by flow cytometry, and levels of tumor necrosis factor alpha, transforming growth factor beta, and matrix metalloproteinase-2 and MMP-9 by immunocytochemistry. RESULTS: Doxorubicin + ozone treatment enhanced viability of L929 (p<0.01) but reduced viability of MCF-7 compared to only doxorubicinapplied cells without ozone treatment (p<0.001). This combined treatment also enhanced apoptotic effect of doxorubicin on MCF-cells (p<0.001), but not on L929. It significantly increased all protein levels of L929 compared with those of other groups (p<0.05 for tumor necrosis factor alpha and MMP-2; p<0.01 for transforming growth factor beta and MMP-9). This treatment reversed the effect of doxorubicin on tumor necrosis factor alpha levels and considerably reduced MMP-2 and MMP-9 levels of MCF-7 compared with those of control group (p<0.01 and p<0.001, respectively). CONCLUSION: Ozone treatment potentiated the apoptotic and anticancer activities of doxorubicin in MCF-7 cells and showed repairing and healing effect on healthy fibroblast cells, which were damaged from cytotoxic effects of chemotherapeutic agent. MCF-7 cells may acquire sensitivity against the doxorubicin combined with ozone treatment through activating tumor necrosis factor alpha, MMP-2, and MMP-9 expressions.

show abstract

Ellagic acid enhances antitumor efficacy of temozolomide in an in vitro glioblastoma model

Cited by 9 publications

References 29 publications

Antitumor activity of irinotecan with ellagic acid in C6 glioma cells

Antitumor activity of irinotecan with ellagic acid in C6 glioma cells

Advances in Dietary Phenolic Compounds to Improve Chemosensitivity of Anticancer Drugs

Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line

Contact Info

Product

Resources

About