ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Biernacki, B.

doi:10.1515/bvip-2015-0052

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…The chloramphenicol residues were determined by enzyme-linked immunosorbent assay (ELISA) method. The analysis was performed in two steps: sample preparation and immunoenzymatic analysis [ 73 , 74 , 75 ].…”

Section: Methodsmentioning

confidence: 99%

The Influence of Chemical Contaminants on the Physicochemical Properties of Unifloral and Multifloral Honey

Scripcă

Amariei

2021

Foods

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The aim of this study was to evaluate and compare the effect of antibiotic and pesticide residues on the physicochemical properties of unifloral and multifloral honey. The mineral elements content of honey was analyzed and correlated with antibiotic and pesticide residues, and a positive correlation was found between manganese and neonicotinoids. Potassium was found to be the most abundant mineral compound. Correlations were found between mineral content, color, and the content of antibiotic and pesticide residues of honey. In meadow honey, residues of antibiotics and pesticides were undetectable. In some of the other types of honey, the maximum residue limits regulated by European legislation were exceeded. Endosulfan residue was found in mint and rapeseed, honey with 0.42 and 5.14 ng/g, respectively. Neonicotinoids were found in 27% of the analyzed honey samples. Chloramphenicol was identified only in rapeseed honey, with concentrations ranging from 0.2 ng/g to 0.8 ng/g. Nitrofurans were found in 14%, and nitroimidazoles were found in 6% of the analyzed samples. According to EU legislation that is in force, the use of antibiotics in beekeeping is not allowed. The MRLs for neonicotinoids are 50 ng/g, and for coumaphos, the maximum limit is 100 ng/g. For the other pesticide residues, the maximum limit is 10 ng/g. The results of statistical analysis obtained using principal component analysis (PCA) showed a major difference in the levels of contamination of raspberry and meadow honey and the other types of honey.

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Section: Methodsmentioning

confidence: 99%

The Influence of Chemical Contaminants on the Physicochemical Properties of Unifloral and Multifloral Honey

Scripcă

Amariei

2021

Foods

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“…Several analytical methods have been employed for analysis and determination of CAP in aquatic products, including Gas Chromatography-Mass Spectrometry (GC-MS), 3 Liquid Chromatog-raphy-Mass Spectrometry (LC-MS), 4,5 En-zyme-Linked Immunosorbent Assay (ELISA), 6,7 High Performance Liquid Chromatography (HPLC). 8 However, these methods involved special and high cost equipment.…”

mentioning

confidence: 99%

Method Validation of Chloramphenicol Analysis in the Shrimp Based on Diazotization Reaction

Wafi¹,

Supriyanto²,

Tjahjandarie³

2020

IJPST

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A simple, rapid and precise spectrophotometric method has been developed and validated for the determination of Chloramphenicol (CAP) in the shrimp based on diazotization reaction at room temperature. The CAP was reduced by zinc powder and the diazotization reaction was carried out in the presence of NaNO2, bismuth nitrate pentahydrate as catalyst. The 2-napthol used as coupling agent to form a red-violet solution and the absorbance of azo dye solution was measured by UV-Vis spectrophotometer at 554 nm. The method validation parameters including linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) have been investigated. The correlation coefficient (R2) was 0.996 for concentration range 0.70 -4.65 µg/mL. The LOD and LOQ were 0.36 µg/ mL and 1.19 µg/mL. Accuracy and precision of the method were performed by spiking of CAP in the shrimp sample at concentration 1.16; 2.33; 3.49 µg/mL. Analysis result showed that the accuracy and precision of the method were 92.77-97.37 % and 0.21-2.39 % respectively.

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“…In recent years, various high-sensitive detection methods for CAP have been extensively studied, including enzyme-linked immunoassay (ELISA), 3 high-performance liquid chromatography−mass spectrometry (HPLC−MS), 4 conventional electrochemical method, 5 and molecularly imprinted electrochemical methods. 6 While these methods exhibit high sensitivity, most require complex operations, precision, and long detection times, making them unsuitable for real-time field detection.…”

Section: ■ Introductionmentioning

confidence: 99%

Comprehensive SERS–LFIA Platform for Ultrasensitive Detection and Automated Discrimination of Chloramphenicol Residues in Aquatic Products

Zhao,

Lin,

et al. 2024

ACS Appl. Nano Mater.

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With the increasing misuse of antibiotic drugs, the convenient and ultrasensitive detection of chloramphenicol (CAP) is essential for ensuring food safety and human health. In this study, a comprehensive platform was developed integrating detection and discrimination capabilities by combining surfaceenhanced Raman scattering (SERS) with lateral flow immunoassay (LFIA) technology, along with a support vector machine (SVM) model for data analysis, to detect CAP residues in aquatic products. The CAP-specific antibody-modified gold nanostars (Au NSs) were synthesized as SERS−LFIA nanoprobes, achieving an ultralow limit of detection (LOD) of 10 pg/mL and a theoretical LOD of 1.6 pg/mL for simulated aquatic samples. This sensitivity surpasses that of the currently available commercial strips and demonstrates high specificity. Additionally, the automated discrimination model exhibited high sensitivity (95.83%) and specificity (96.43%) as well as an accuracy of 96% on the test set, marking an 8% improvement over traditional Raman intensity discrimination methods. This developed platform is a simple, fast, and automated technique that eliminates the need for sample pretreatment, offering a promising approach for the trace detection of CAP in aquatic products.

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ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Cited by 6 publications

References 7 publications

The Influence of Chemical Contaminants on the Physicochemical Properties of Unifloral and Multifloral Honey

The Influence of Chemical Contaminants on the Physicochemical Properties of Unifloral and Multifloral Honey

Method Validation of Chloramphenicol Analysis in the Shrimp Based on Diazotization Reaction

Comprehensive SERS–LFIA Platform for Ultrasensitive Detection and Automated Discrimination of Chloramphenicol Residues in Aquatic Products

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