ELISA qualitative screening of chloramphenicol in muscle, eggs, honey and milk: method validation according to the Commission Decision 2002/657/EC criteria

Scortichini, Giampiero; Annunziata, Loredana; Haouet, M. Naceur; Benedetti, Fabio; Krusteva, I.; Galarini, Roberta

doi:10.1016/j.aca.2004.12.004

Cited by 86 publications

(37 citation statements)

References 11 publications

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“…Existing analytical methods for the determination of CAP include immunoassays (IAs) [2][3][4], microbiological methods [5], sensors [6], GC [7,8], LC-UV or LC-MS/MS [9][10][11][12], and CE [13][14][15]. Among these, IA and chromatographic assays (as LC-MS/MS) are widely used, although they have some drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

et al. 2008

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A competitive immunoassay using CE with an LIF detector was developed for the detection of chloramphenicol (CAP). The method was based on the competitive reactions between fluorescently labeled CAP hapten and free CAP, with a limited amount of anti-CAP antibody. The poly(N-isopropylacrylamide) (pNIPA) hydrogel was added in the separation buffer as a dynamic modifier to reduce adsorption and enhance reproducibility. The linear range and LOD for CAP were 0.008-5 microg/L and 0.0016 microg/L, respectively. An ELISA using the same immuno-reagents was also developed for the analysis of CAP, with an LOD of 0.03 microg/L. The sensitivity of this CE immunoassay (CEIA)-LIF was almost 20 times greater than that of the ELISA. Using CEIA-LIF, equilibrium was reached in 15 min and the analytical results were obtained within 5 min by CE separation. Sample preparation for CEIA-LIF was not time-consuming and the matrix effect was easy to remove. An LOD of 0.1 microg/kg CAP in food matrices was easily achieved. This method is thus proposed as a fast and sensitive means of detecting trace amounts of CAP residues in animal-derived foods.

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Section: Introductionmentioning

confidence: 99%

Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

et al. 2008

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“…1,6 No maximum residue limit (MRL) of chloramphenicol in animalderived food has been established, because its toxic effects are not dose-dependent, but rather related to the hypersensitivity of certain individuals. 7 Several analytical methods have been reported for the determination of chloramphenicol in various samples, such as shrimp, 3,[8][9][10][11] seafood, meat, 7,12-15 eggs, 13 milk, 4,13 honey, 12,13,15 animal feeds, 5 urine, serum [14][15][16] and pharmaceutical formulations [17][18][19][20][21][22] based on liquid chromatography (LC), 5,12 liquid chromatography-mass spectrometry (LC-MS), 3,[7][8][9][10][11]14,15 gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), 3,12,14 capillary zone electrophoresis, 16,17 enzyme-linked immunosorbent assay (ELISA), 3,13 spectrophotometry, 18,19 and chemiluminescence. [20][21]…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Analysis of Chloramphenicol Using Boron-doped Diamond Electrode Applied to a Flow-Injection System

2008

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“…at 95 % confidence level. If t value is greater than t crit , the investigated variable shows a significant influence, and the method is not sufficiently robust against the chosen modification [36].…”

Section: Methods Validationmentioning

confidence: 98%

Simultaneous Detection of Glabridin, (−)-α-Bisabolol, and Ascorbyl Tetraisopalmitate in Whitening Cosmetic Creams Using HPLC-PAD

Jeon

Kim

et al. 2016

Chromatographia

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α-bisabolol, and ascorbyl tetraisopalmitate, respectively. The calibration curves showed good linearity with determination coefficients (R 2 ) ≥ 0.999. The mean recoveries were ranged between 89.8 and 103.9 % with relative standard deviations (RSDs) <5 %. The intra-and inter-day precision was <2 %. The limits of detection (LODs) were 0.03, 0.4, and 4.02 μg mL −1 for glabridin, (−)-α-bisabolol, and ascorbyl tetraisopalmitate, respectively. The method was successfully applied for monitoring 11 market samples, in which glabridin was quantified in the range of 17.5-25 mg 100 g −1 , (−)-α-bisabolol in the range of 25.1-677 mg 100 g −1 , and 140.6-291.5 mg 100 g −1 for ascorbyl tetraisopalmitate. The proposed analytical method is simple, sensitive, and versatile and can be used for the quantification of lipophilic compounds in cosmetics in a single chromatographic run.

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ELISA qualitative screening of chloramphenicol in muscle, eggs, honey and milk: method validation according to the Commission Decision 2002/657/EC criteria

Cited by 86 publications

References 11 publications

Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

Electrochemical Analysis of Chloramphenicol Using Boron-doped Diamond Electrode Applied to a Flow-Injection System

Simultaneous Detection of Glabridin, (−)-α-Bisabolol, and Ascorbyl Tetraisopalmitate in Whitening Cosmetic Creams Using HPLC-PAD

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