Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

Zhang, Can; Wang, Shuo; Fang, Guozhen; Zhang, Yan; Jiang, Liqin

doi:10.1002/elps.200800188

Cited by 30 publications

(15 citation statements)

References 31 publications

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“…The sensitivity of dcELISA herein is better than the previous report which had an IC 50 value of 0.3 ng/ml (Fodey, Murilla, Cannavan, & Elliott, 2007). In addition, the dcELISA detection limit herein is lower than the previous reported which had the detection limit of 0.03 ng/ml (Zhang, Wang, Fang, Zhang, & Jiang, 2008).…”

Section: Production and Characterization Of Antibodiescontrasting

confidence: 66%

Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food

Wang

Liu

et al. 2020

Journal of Food Safety

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Antibody specific to chloramphenicol (CAP) was produced from rabbit that had been immunized with CAP‐keyhole limpet hemocyanin (KLH). Using the antibodies, we established a sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) and a gold nanoparticle immunochromatographic strip (immunostrip) for detection CAP in food samples. In the dcELISA, CAP at levels of 0.15 ng/ml causes 50% inhibition (IC50) of the binding of CAP‐horseradish peroxidase to the antibodies. The overall analytical recoveries of CAP (0.25–100 ng/g) added to the honey or milk samples in the dcELISA were 81.9 and 73.7%, respectively. Onsite determination of CAP was accomplished by immunostrips with a detection limit of 0.5 ng/ml and completed within 10 min. Carefully studying 10 honey and 6 milk samples using the dcELISA and immunostrip indicated that all examined samples were negative for CAP. The presented dcELISA and immunostrip methods are sensitive enough for the rapid determination of CAP in the samples.

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Section: Production and Characterization Of Antibodiescontrasting

confidence: 66%

Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food

Wang

Liu

et al. 2020

Journal of Food Safety

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“…The developed method exhibits the best LOD value among other flow methods with different detections, and better values of LOD were reported only for sophisticated capillary electrophoretic method with LIF detection [34], and also one of reported surface plasmon resonance method [37]. Table 3 does not include applications of LC/MS methods in food and biological material analysis, where LOD values are evaluated in terms of content of CAP in analyzed material.…”

Section: Discussionmentioning

confidence: 99%

Flow-Injection Preconcentration of Chloramphenicol Using Molecularly Imprinted Polymer for HPLC Determination in Environmental Samples

Kowalski

Poboży

Trojanowicz

2011

Journal of Automated Methods and Management in Chemistry

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The residue of antibiotic chloramphenicol (CAP) is important issue for food quality control and also for the environmental monitoring. It is banned for use in food-producing animals and has very limited use in human medicine, because of its severe impact on human health. Determination of trace level of CAP in environmental samples requires a very sensitive analytical method and efficient preconcentration procedure. CAP can be efficiently preconcentrated in flow-injection system using flow-through reactor packed with molecularly imprinted polymer (MIP), but determination of CAP in eluate from MIP requires the application of chromatographic separation, which was made in reversed-phase HPLC system with UV detection. In optimized conditions the limit of detection for 100 mL sample in HPLC with offline preconcentration on MIP was evaluated as 0.66 mg/L. In hyphenated FIA-HPLC system with zone sampling the LOD for developed method was evaluated as 15 ng/L, which indicates the possibility of using it for analysis of environmental samples.

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“…Particularly, the assay time is shorter because the immunoreaction occurring in solution is more rapid as a result of solution phase kinetics compared to tradition IA. Till date, CEIA‐LIF has been employed to detect a wide range of compounds including proteins [], pesticides and veterinary drugs [], hormones [] and so on. However, MTMC detection using CEIA with an LIF detector has not been studied so far.…”

Section: Introductionmentioning

confidence: 99%

Determination of metolcarb in food by capillary electrophoresis immunoassay with a laser‐induced fluorescence detector

Liu¹,

Fang²,

Deng³

et al. 2012

Electrophoresis

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A capillary electrophoresis immunoassay (CEIA) was developed for the determination of trace metolcarb (MTMC) in food. The method was based on the competitive reactions between fluorescently labeled MTMC tracer and free MTMC with a limited amount of anti-MTMC antibody and the separation and determination by CE with LIF detector. A fluorescent reagent, FITC was labeled on MTMC to construct an immunofluorescent probe. CEIA experimental parameters such as the pH value and concentration of the running buffer and separation voltage as well as incubation time were systematically investigated. Under the optimized conditions, fluorescently labeled antigen and antibody bound could be well separated within 3 min using Na₂B₄O₇/NaH₂PO₄ buffer (20:10 mmol/L, pH 9.0) for background electrolyte, 20 kV for the separation voltage, and 20°C for the column temperature. The linear range of the method was 0.25-50.0 μg/L with LOD 0.07 μg/L. The RSD for relative migration time and relative fluorescence intensity ratio were 2.90% (intraday) and 4.73% (intraday), respectively. The proposed method has been applied to determine the residue of MTMC in food samples with the satisfactory recovery.

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Competitive immunoassay by capillary electrophoresis with laser‐induced fluorescence for the trace detection of chloramphenicol in animal‐derived foods

Cited by 30 publications

References 31 publications

Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food

Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food

Flow-Injection Preconcentration of Chloramphenicol Using Molecularly Imprinted Polymer for HPLC Determination in Environmental Samples

Determination of metolcarb in food by capillary electrophoresis immunoassay with a laser‐induced fluorescence detector

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