ELISA of 6-beta-hydroxycortisol in human urine: Diurnal variations and effects of antiepileptic therapy

The increase in urinary excretion of 6 beta‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction.

“…In contrast, free urinary cortisol was not significantly modified by rifampicin treatment. These results, which are in close agreement with those reported previously (Roots et al, 1979;Zhiri et al, 1986;Desage et al, 1987) constituted the first step of our work. Following this in vivo study we next focussed on enzymatic assays and cytochromes P450 measurement in microsomes prepared from liver biopsies.…”

“…The h.p.l.c. method we used was validated on the basis of the following points: (1) Our data for healthy adults were in good agreement with previously published values (Dumont et al, 1984;Nakamura & Yakata, 1985;Hosoda et al, 1981;Kishida & Fukushima, 1983;Nahoul et al, 1982;Park, 1978;Zhiri et al, 1986;Desage et al, 1987). (2) In addition we had the opportunity, in a separate study, to perform both our h.p.l.c.…”

“…(2) In addition we had the opportunity, in a separate study, to perform both our h.p.l.c. assay and an immunoassay developed by Zhiri et al (1986) on the same samples; an excellent correlation was found (r = 0.954, P < 0.001, n = 20) although the two methods are totally different and differ in both specificity and sensitivity. Some samples of the 613-hydroxycortisol related peak were collected during the h.p.l.c.…”

“…This finding is consistent with early papers reporting that 6,B-hydroxycortisol urinary excretion is not associated with debrisoquine polymorphism which is related to a genetic defect affecting P450IID6 gene expression (Gonzalez et al, 1988) and is not affected by polycyclic aromatic hydrocarbons (P450IA1 and P4501A2 inducers) produced by cigarette smoking (Vestal et al, 1987). On the other hand, anticonvulsants known to be inducers of the P450IIIA subfamily in vivo in laboratory animals (phenobarbitone) or in human hepatocyte cultures (phenobarbitone, carbamazepine, phenytoin, P. Maurel, unpublished observations) can also increase 61-hydroxycortisol urinary excretion in vivo in man (Park, 1981;Ohnhaus & Park, 1979;Moreland et al, 1982;Zhiri et al, 1986).…”

“…Various h.p.l.c. systems, as well as immunoassays, have been described for this measurement (Dumont et al, 1984;Nakamura & Yakata, 1985;Hosoda et al, 1981;Kishida & Fukushima, 1983;Nahoul et al, 1982;Park, 1978;Zhiri et al, 1986;Desage et al, 1987). The h.p.l.c.…”