Elimination of Vibrio parahaemolyticus biofilms on crab and shrimp surfaces using ultraviolet C irradiation coupled with sodium hypochlorite and slightly acidic electrolyzed water

Roy, Pantu Kumar; Mizan, Md. Furkanur Rahaman; Hossain, Md. Iqbal; Han, Noori; Begum, Shameem Ara; Ashrafudoulla, Md.; Toushik, Sazzad Hossen; Shim, Won‐Bo; Kim, Young‐Mog; Ha, Sang‐Do

doi:10.1016/j.foodcont.2021.108179

Cited by 50 publications

(18 citation statements)

References 63 publications

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“…Listeria monocytogenes was obtained from American Type Culture Collection (Manassas, VA, USA), with strains ATCC19113, ATCC19117, and ATCC15313, and used for the biofilm forming assays. The bacteria were cultured in tryptic soy broth (TSB, BD Difco, Franklin Lakes, NJ, USA) at 30 °C for 24 h, followed by another sub-culture at 18 h [ 49 ]. The cultures were centrifuged (11,000× g for 10 min) and washed two times with phosphate-buffered saline (PBS; Oxoid, Basigstoke, Winchester, UK).…”

Section: Methodsmentioning

confidence: 99%

“…With few modifications, sample preparation was done as explained in our earlier investigations [ 49 ]. Using a sterile scissors, latex hand gloves (HG, Komax Industrial Co., Ltd., Seoul, Korea) were cut into 2 × 2 cm 2 coupons, and silicon rubber and stainless-steel coupons (2 cm × 2 cm × 0.1 cm) were also used.…”

Section: Methodsmentioning

confidence: 99%

“…Following the removal of any dirt, the coupons were cleaned with sterile, distilled water (DW). The coupons were sterilized by autoclaving (121 °C for 15 min) and dried overnight [ 49 ]. The coupons were completely submerged into 50 mL Falcon tubes containing 10 mL TSB, inoculated with final concentrations (100 µL) of bacteria (10 5 CFU/mL), and then incubated for 24 h at 30 °C without shaking for further experiment.…”

Section: Methodsmentioning

confidence: 99%

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The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

2022

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Listeria monocytogenes is the species of foodborne pathogenic bacteria that causes the infection listeriosis. The food production chain employs various methods to control biofilms, although none are completely successful. This study evaluates the effectiveness of quercetin as a food additive in reducing L. monocytogenes mixed cultures (ATCC19113, ATCC19117, and ATCC15313) biofilm formation on stainless steel (SS), silicon rubber (SR), and hand glove (HG) coupons, as well as tests its antimicrobial activities. With a minimum inhibitory concentration (MIC) of 250 µg/mL, the tested quercetin exhibited the lowest bactericidal action with no visible bacterial growth. In contrast, during various experiments in this work, the inhibitory efficacy of quercetin at sub-MICs levels (1/2, 1/4, and 1/8 MIC) against L. monocytogenes was examined. A control group was not added with quercetin. The current study also investigates the effect of quercetin on the expression of different genes engaged in motility (flaA, fbp), QS (agrA), and virulence (hlyA, prfA). Through increasing quercetin concentration, swarming and swimming motility, biofilm formation, and expression levels of target genes linked to flagella motility, virulence, and quorum-sensing were all dramatically reduced. Quercetin (0–125 μg/mL) was investigated on the SS, SR, and HG surfaces; the inhibitory effects were 0.39–2.07, 0.09–1.96 and 0.03–1.69 log CFU/cm2, respectively (p < 0.05). Field-emission scanning electron microscopy (FE-SEM) corroborated the findings because quercetin prevented the development of biofilms by severing cell-to-cell contacts and inducing cell lysis, which resulted in the loss of normal cell shape. Our findings suggest that plant-derived quercetin should be used as an antimicrobial agent in the food industry to control the development of L. monocytogenes biofilms. These outcomes suggest that bacterial targets are of interest for biofilm reduction, with alternative natural food agents in the food sector along the entire food production chain.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

2022

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“…(Lee & Jang, 2004). Research on microbial degradation through NaOCl has been reported by some studies (Chen et al, 2019; Lee et al, 2011; Oh, 2011; Park et al, 2016; Roy et al, 2021), but most of them are about vegetables and have not been applied to marine products. Therefore, this study investigated the effects of NaOCl treatment (at a chlorine concentration of 100–1000 mg/L) against HNoV GII.4 infectivity in fresh Manila clams assessed via PMA with RT‐qPCR.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of sodium hypochlorite on the potential infectivity of human norovirus GII .4 using propidium monoazide with RT‐qPCR and quality assessments in Manila clams ( Ruditapes philippinarum )

Min

Jeon

Kim

et al. 2022

Food Processing Preservation

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This study investigated the effect of NaOCl on the inactivation of human norovirus (HNoV) GII.4 infectivity in Manila clams treated with NaOCl solution at 100–1000 mg/L chlorine for 10 min using propidium monoazide (PMA)/RT‐qPCR. HNoV GII.4 detected using non‐PMA/PMA were significantly (p < .05) reduced to 0.15/0.37, 0.33/0.58, 0.59/0.85, 0.69/0.99, and 1.05/1.54 log10copy number/μl upon a 10 min treatment with 100, 300, 500, 700, and 1000 mg/L of NaOCl, respectively. The D‐value of HNoV GII.4 infectivity in non‐PMA and PMA‐treated samples was calculated as 1000 and 714.3 mg/L of NaOCl, respectively, using the first‐order kinetics model. The pH value increased significantly as chlorine concentration increased. Changes in color and appearance in quantitative analysis evaluation did not appear after treatment with 100–1000 mg/L chlorine although all sensorial parameters were above 4 points in all NaOCl treatments. The highest score of overall acceptability was observed in 100–500 mg/L chlorine‐treated Manila clams. Practical applications The results suggest that PMA/RT‐qPCR could help distinguish HNoV infectivity by negatively affecting clam flavor following NaOCl treatment with 1000 mg/L of chlorine. Moreover, the inactivation kinetics of chlorine against HNoV might provide further information for Manila clam processing and distribution.

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Comparison of the efficacy of physical and chemical strategies for the inactivation of biofilm cells of foodborne pathogens

Ban

Kim

Kang

et al. 2023

Food Sci Biotechnol

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Elimination of Vibrio parahaemolyticus biofilms on crab and shrimp surfaces using ultraviolet C irradiation coupled with sodium hypochlorite and slightly acidic electrolyzed water

Cited by 50 publications

References 63 publications

The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

Effects of sodium hypochlorite on the potential infectivity of human norovirus GII .4 using propidium monoazide with RT‐qPCR and quality assessments in Manila clams ( Ruditapes philippinarum )

Comparison of the efficacy of physical and chemical strategies for the inactivation of biofilm cells of foodborne pathogens

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