Elimination of Transmissible Drug-resistance by Treatment with Acriflavin

Mitsuhashi, Susumu; Harada, Kenji; Kameda, Mitsuo

doi:10.1038/189947a0

Cited by 83 publications

(22 citation statements)

References 4 publications

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“…This plasmid produces sex pili constitutively. Acridines have little, if any, effect in elimination of R factors (Mitsuhashi, Harada & Kameda, 1961;Watanabe & Ogata, 1966). We have found that F factors can be eliminated equally efficiently from cells in which pili are produced constitutively and from those in which pilus production is repressed by the presence of anJi+ R factor.…”

Section: Introductionmentioning

confidence: 99%

Two Modes of 'Curing' Transmissible Bacterial Plasmids

Salisbury¹,

Hedges²,

Datta³

1972

Journal of General Microbiology

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Section: Introductionmentioning

confidence: 99%

Two Modes of 'Curing' Transmissible Bacterial Plasmids

Salisbury¹,

Hedges²,

Datta³

1972

Journal of General Microbiology

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“…We noted early (J. Ciak, S. Wormley, and F. E. Hahn, Bacteriol. Proc., p. 67, 1969) that among bacterial plasmid eliminating chemicals one category was comprised of substances that bind to deoxyribonucleic acid (DNA) by intercalation (15,20,30,31). After it had been shown that Rfactors chemically are DNA (11) and that bacteria from which all resistance determinants had been eliminated no longer contained -factor DNA (8,25), we assumed that intercalative eliminators of R-factors act by selective complexation with plasmid DNAs and, thereby, selectively inhibit their replication.…”

mentioning

confidence: 99%

Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds

Hahn

Ciak

1976

Antimicrob Agents Chemother

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Eighteen deoxyribonucleic acid (DNA)-complexing compounds, among them 15 intercalative substances, and, additionally, nalidixic acid eliminated with different frequencies four antibiotic resistance determinants from the R-factor R1, carried by Salmonella typhimurium . Eliminating concentrations did not inhibit growth of the bacteria. The most active compound was “nitroacridine II” {1-diethylamino-3-[(6-nitro-9-acridinyl)amino]propanol}. When 14 compounds which had been tested at a standard concentration of 10 −4 M were listed according to decreasing activities of elimination of the four resistance determinants, a nearly consistent activity sequence was revealed. Frequencies of elimination of the kanamycin resistance determinant correlated directly with the binding of compounds to DNA, i.e., with end points of their displacement of methyl green from the methyl green-DNA complex. We propose that the observed eliminations resulted from selective toxicity for plasmidic template DNA and inhibitions of R-factor replication.

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“…Acriflavin, which was used in isolating E. coli F-17 S' Ts, is a plasmid curing agent (Mitsuhashi et al, 1961). However, plasmid profiles of F-17 Sr and F-17 S' Ts were identical ( Fig.…”

Section: Plasmid Profilesmentioning

confidence: 99%

Altered Colonizing Ability for Mouse Large Intestine of a Surface Mutant of a Human Faecal Isolate of Escherichia coli

1983

Microbiology

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Elimination of Transmissible Drug-resistance by Treatment with Acriflavin

Cited by 83 publications

References 4 publications

Two Modes of 'Curing' Transmissible Bacterial Plasmids

Two Modes of 'Curing' Transmissible Bacterial Plasmids

Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds

Altered Colonizing Ability for Mouse Large Intestine of a Surface Mutant of a Human Faecal Isolate of Escherichia coli

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