Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds

Hahn, Fred E.; Ciak, Jennie

doi:10.1128/aac.9.1.77

Cited by 41 publications

(16 citation statements)

References 28 publications

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“…Nalidixic acid has been reported to eliminate plasmid Rl (12) but not several other plasmids (3,4). Oxolinic acid has not been evaluated as a curing agent.…”

mentioning

confidence: 99%

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Hooper¹,

Wolfson²,

McHugh³

et al. 1984

Antimicrob Agents Chemother

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The ability of novobiocin to eliminate (cure) the wild-type plasmid pMG110 from Escherichia coli has been compared with that of other inhibitors of the gyrase B subunit and of the gyrase A subunit. Novobiocin eliminated pMG110, producing over 99% plasmid loss at concentrations two-to eightfold below the MIC for bacterial growth. Structurally related compounds (clorobiocin, coumermycin Al, isobutyryl novenamine, and decarbamyl novobiocin) varied in their ability to eliminate pMG110. Higher concentrations of drugs were required to eliminate pMG110 from a gyrB(Cour) strain, implicating DNA gyrase in the curing phenomenon. For these drugs, the ratio of the concentration effecting maximal plasmid elimination to the MIC varied from 0.16 to 1.1, indicating that curing cannot be explained simply by inhibition of a pool of DNA gyrase equally available for replication of the bacterial chromosome and the plasmid DNA molecule. Inhibitors of the gyrase A subunit, nalidixic acid and oxolinic acid, eliminated pMG10 only to variable low levels. The differences in the ability of the gyrase A and B subunit antagonists to eliminate plasmids are discussed.

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“…Nalidixic acid has been reported to eliminate plasmid Rl (12) but not several other plasmids (3,4). Oxolinic acid has not been evaluated as a curing agent.…”

mentioning

confidence: 99%

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Hooper¹,

Wolfson²,

McHugh³

et al. 1984

Antimicrob Agents Chemother

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“…We have shown that deoxyribonucleic acidintercalating compounds eliminate resistance determinants from R-plasmid Rl carried by Salmonella typhimurium LT-2 and, hence, restore antibiotic susceptibility to this bacterium (2). The purpose of this work was to investigate if sensitization of S. typhimurium R1+ to antibiotics and the bactericidal actions of such antibiotics can occur concurrently in the same bacterial cultures.…”

Section: -6 M)mentioning

confidence: 99%

“…The R-plasmid Rl carries a determinant of resistance to ampicillin (2). In serial dilution test tube assays, S. typhimurium R1+ incubated overnight was resistant to 2,500 ug of ampicillin per ml.…”

Section: -6 M)mentioning

confidence: 99%

Bactericidal Effects of Combinations of Ampicillin with Anti-R-Plasmid Compounds on Salmonella typhimurium R1 ⁺

Hahn

Ciak

1977

Antimicrob Agents Chemother

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“…To find out the location (chromosomal or extra chromosomal) of gene(s) responsible for the resistance to drugs, when plasmid curing experiments were performed using both physical and chemical agents; it was observed that, curing was most effective with ethidium bromide, followed by acridine orange (Hahn and Ciak, 1976;Tahur, 2006).…”

Section: Elimination Prevention and Control Of Microbial Resistancementioning

confidence: 99%

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

M¹,

Bagde²

2017

Afr. J. Biotechnol.

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Antimicrobial resistance developed in several pathogens poses an increasing threat to human health across the world. No country can escape from the medical and economic impacts from this serious problem. Although the antibiotic resistance is not a new phenomenon, the current magnitude and speed with which it is developing is a cause for the global concern including in India. There are so many common diseases resulting from the microorganisms such as blood stream infections, urinary tract infections, post-operative wound infections and intra-abdominal infections. In this review the antimicrobial susceptibility or resistance of Enterobacter towards antimicrobial agents and heavy metals, viz. ceftazidime, moxifloxacin, nalidixic acid, sulfamethaxazole, and nickel and lead is discussed briefly along with other antimicrobials and heavy metals. The mechanisms behind the resistance by Enterobacter was analyzed and evaluated by many workers after using currently employed susceptibility testing methods for Enterobacter spp. There are some factors influencing mode of action of fluoroquinolones, quinolones and sulfamethaxazole. History, classification, identification, clinical features and treatment of infections and the epidemiology of antimicrobials (drugs and heavy metals) resistance by the Enterobacter spp. is included in this review. Now a day, antimicrobial resistance is common in hospitals where acquired infections can be perilous. This situation compels scientists to synthesize new antibiotics and treatment modalities. Enterobacter causes nosocomial infections. It is ubiquitous and can survive on skin and dry surfaces and replicate in contaminated fluids. Numerous outbreaks have been described. Various mechanisms have been adapted by microorganisms to resist toxicity of antimicrobials. Antimicrobial drugs may be rendered inactive or ineffective by the major ways such as barrier to antibiotic entry into the bacterial cell, prevention of the antibiotic from reaching the target, often by extrusion, alteration of the target of the drug and inactivation of the antibiotic by modification or destruction. In addition, bacteria may be able to bypass the metabolic pathway affected by a particular drug or may be able to overproduce an enzyme that is inhibited by the drug action, more than one mechanism may operate at any given time

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Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds

Cited by 41 publications

References 28 publications

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Bactericidal Effects of Combinations of Ampicillin with Anti-R-Plasmid Compounds on Salmonella typhimurium R1 ⁺

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

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Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds

Cited by 41 publications

References 28 publications

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase

Bactericidal Effects of Combinations of Ampicillin with Anti-R-Plasmid Compounds on Salmonella typhimurium R1 +

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

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Bactericidal Effects of Combinations of Ampicillin with Anti-R-Plasmid Compounds on Salmonella typhimurium R1 ⁺