Elimination of high-risk human papillomavirus type HPV16 infection by ‘Praneem’ polyherbal tablet in women with early cervical intraepithelial lesions

Shukla, Shirish; Bharti, Alok C.; Hussain, Showket; Mahata, Sutapa; Hedau, Suresh; Kailash, Uma; Kashyap, Veena; Bhambhani, Suresh; Roy, Meera; Batra, Swaraj; Talwar, G. P.; Das, Bhudev C.

doi:10.1007/s00432-009-0617-1

Cited by 76 publications

(21 citation statements)

References 25 publications

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“…Currently, there is no HPV specific advanced therapy for treatment of cervical cancer. Recent studies have shown that anti-oxidants and herbal derivatives may be effective against prevailing HR-HPV infection but these leads are yet to prove their efficacy in preclinical and subsequent clinical studies [26-29]. …”

Section: Introductionmentioning

confidence: 99%

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

Mahata

Maru

Shukla

et al. 2012

BMC Complement Altern Med

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Background Bryophyllum pinnata ( B. pinnata ) is a common medicinal plant used in traditional medicine of India and of other countries for curing various infections, bowel diseases, healing wounds and other ailments. However, its anticancer properties are poorly defined. In view of broad spectrum therapeutic potential of B. pinnata we designed a study to examine anti-cancer and anti-Human Papillomavirus (HPV) activities in its leaf extracts and tried to isolate its active principle. Methods A chloroform extract derived from a bulk of botanically well-characterized pulverized B . pinnata leaves was separated using column chromatography with step- gradient of petroleum ether and ethyl acetate. Fractions were characterized for phyto-chemical compounds by TLC, HPTLC and NMR and Biological activity of the fractions were examined by MTT-based cell viability assay, Electrophoretic Mobility Shift Assay, Northern blotting and assay of apoptosis related proteins by immunoblotting in human cervical cancer cells. Results Results showed presence of growth inhibitory activity in the crude leaf extracts with IC 50 at 552 μg/ml which resolved to fraction F4 (Petroleum Ether: Ethyl Acetate:: 50:50) and showed IC 50 at 91 μg/ml. Investigations of anti-viral activity of the extract and its fraction revealed a specific anti-HPV activity on cervical cancer cells as evidenced by downregulation of constitutively active AP1 specific DNA binding activity and suppression of oncogenic c-Fos and c-Jun expression which was accompanied by inhibition of HPV18 transcription. In addition to inhibiting growth, fraction F4 strongly induced apoptosis as evidenced by an increased expression of the pro-apoptotic protein Bax, suppression of the anti-apoptotic molecules Bcl-2, and activation of caspase-3 and cleavage of PARP-1. Phytochemical analysis of fraction F4 by HPTLC and NMR indicated presence of activity that resembled Bryophyllin A. Conclusions Our study therefore demonstrates presence of anticancer and anti-HPV an activity in B . pinnata leaves that can be further exploited as a potential anticancer, anti-HPV therapeutic for treatment of HPV infection and cervical cancer.

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Section: Introductionmentioning

confidence: 99%

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

Mahata

Maru

Shukla

et al. 2012

BMC Complement Altern Med

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“…We had earlier made a microbicide named "Praneem" which had undergone similar trials in women at early stage of HPV 16 infection as reported elsewhere [7]. After 30 days of use of Praneem, 6 out of 10 subjects showed the elimination of HPV 16.…”

Section: Discussionmentioning

confidence: 91%

BASANT, a Polyherbal Safe Microbicide Eliminates HPV-16 in Women with Early Cervical Intraepithelial Lesions

Talwar¹,

Sharma²,

Singh³

et al. 2015

JCT

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Carcinoma cervix is a major cancer of women killing 510,000 women every year worldwide. Human papilloma virus (HPV) infection of cervical cells initiates the transformation of the cervical cells to malignant stage. HPV-16 is the most frequent type of HPV causing these changes. We report here the elimination of HPV-16 from the infected cells of all (11/11) women positive for HPV-16 by 30 intra-vaginal intakes of BASANT.

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“…Based on safety of herbal ingredients and their general anti-microbicidal action, a few poly-herbal formulations or mixtures based on herbal derivatives have been evaluated in clinical studies. Praneem, a combination of leaf extract of Azadirachta indica, saponins, purified extracts of Emblica officinalis, Mentha citrata oil, and extracts of Aloe vera gel was examined in HPV infected women in a placebo controlled clinical study (208). One course of thirty day intra-vaginal application of Praneem polyherbal tablet in women with early cervical intraepithelial lesions resulted in elimination of HPV16 infection in 60% of the women (6/10).…”

Section: Amongmentioning

confidence: 99%

Therapeutic startegies for human papillomavirus infection and associated cancers

et al. 2018

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Human papillomavirus (HPV) infection is linked to development of cancer of cervix, vagina, vulva, penis, ano-genital and non-genital oro-pharyngeal sites. HPV being a sexually transmitted virus infects both genders equally but with higher chances of pathological outcome in women. In the absence of organized screening programs, women report HPV-infected lesions at relatively advanced stages where they are subjected to standard treatments that are not HPV-specific. HPV infection-driven lesions usually take 10-20 years for malignant progression and are preceded by well-characterized pre-cancer stages. Despite availability of window for pharmacological intervention, therapeutic that could eradicate HPV from infected lesions is currently lacking. A variety of experimental approaches have been made to address this lacuna and there has been significant progress in a number of lead molecules which are in different stages of clinical and pre-clinical development. Present review provides a brief overview of the magnitude of the problem and current status of research on promising lead molecules, formulations and therapeutic strategies that showed potential to translate to clinically-viable HPV therapeutics to counteract this reproductive health challenge.

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Elimination of high-risk human papillomavirus type HPV16 infection by ‘Praneem’ polyherbal tablet in women with early cervical intraepithelial lesions

Abstract: Our results showed for the first time that a 30-day intra-vaginal application of the Praneem can result in elimination of HPV infection from the uterine cervix.

Cited by 76 publications

References 25 publications

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

BASANT, a Polyherbal Safe Microbicide Eliminates HPV-16 in Women with Early Cervical Intraepithelial Lesions

Therapeutic startegies for human papillomavirus infection and associated cancers

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