Elimination of altered erythrocytes in young and old rats

Giuliani, Anna; Graldi, Giuseppe; Unis, Lorenza; Verenini, Marina; Pora, Roberto; Melandri, P; Torboli, M; Bergamini, Carlo M.; Berti, G

doi:10.1007/bf03324381

Cited by 2 publications

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(11). Results of our previous in vivo experiments in rats are also in keeping with the observed decrease in erythropoietic activity following macrophages damage/depletion: prolonged treatment of rats with liposome‐encapsulated Cl 2 MDP caused a slight but significant anemia (25) which might be related to the harmful effect of the preparation on bone marrow macrophages and on their activity in regulating erythropoiesis. However, in these experiments inhibitory signals on erythropoiesis from the periphery due to the impaired removal of aged erythrocytes secondary to spleen and liver macrophage depletion might also have played a role.…”

Section: Discussionsupporting

confidence: 81%

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)

Wiener

Lee

Brown

et al. 2001

European J of Haematology

Self Cite

View full text Add to dashboard Cite

In order to explore the effect on bone marrow macrophages of liposome-encapsulated dichloromethylene diphosphonate (Cl2MDP), mice were injected intravenously with a preparation of such liposomes at a dose known to deplete spleen and liver macrophages. Two days later, the macrophages in the marrow of the femoral bones were quantified by flow cytometry using a macrophage-specific monoclonal antibody (F4/80), and their ultrastructure and phagocytic activity towards zymosan particles was assessed. To determine the effect on erythropoiesis of liposome-encapsulated Cl2MDP-induced changes in bone marrow macrophages, red blood cell parameters and the formation of erythroid burst-forming unit (BFU-E)-derived colonies in vitro were evaluated. In mice injected with liposome-encapsulated Cl2MDP, there was a 54% and 67% decrease in the total number of bone marrow macrophages as compared to uninjected controls and mice treated with empty liposomes, respectively. Moreover, residual macrophages showed an abnormal ultrastructure, with reduced numbers of crystalloid inclusions and increased numbers of large myelin figures. However, the phagocytic activity of these cells was unimpaired or slightly enhanced. In mice injected with liposome-encapsulated Cl2MDP there was an approximately 60% decrease in the percentage and total number of circulating reticulocytes and a 54% reduction in the BFU-E number, demonstrating deregulation of erythropoiesis under conditions of macrophage loss and impairment. The results suggest that mice treated with liposome-encapsulated Cl2MDP are a model for studying the role of macrophages in erythropoiesis.

show abstract

Section: Discussionsupporting

confidence: 81%

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)

Wiener

Lee

Brown

et al. 2001

European J of Haematology

Self Cite

View full text Add to dashboard Cite

show abstract

Binding of anti-spectrin antibodies to red blood cells and vesiculation in various in vivo and in vitro ageing conditions in the rat

Graldi¹,

Veronesi²,

Previato³

et al. 2000

Experimental Gerontology

View full text Add to dashboard Cite

Elimination of altered erythrocytes in young and old rats

Cited by 2 publications

References 3 publications

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)

Binding of anti-spectrin antibodies to red blood cells and vesiculation in various in vivo and in vitro ageing conditions in the rat

Contact Info

Product

Resources

About

Elimination of altered erythrocytes in young and old rats

Cited by 2 publications

References 3 publications

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl2MDP)

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl2MDP)

Binding of anti-spectrin antibodies to red blood cells and vesiculation in various in vivo and in vitro ageing conditions in the rat

Contact Info

Product

Resources

About

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)

Changes in murine bone marrow macrophages and erythroid burst‐forming cells following the intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (Cl₂MDP)