Elicitor-induced in vitro shoot multiplication and steviol glycosides production in Stevia rebaudiana

Rasouli, Diako; Werbrouck, Stefaan; Maleki, Bahram; Jafary, Hossein; Schurdi‐Levraud, Valérie

doi:10.1016/j.sajb.2020.10.023

Cited by 22 publications

(7 citation statements)

References 54 publications

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“…The total content of stevioside both in control and treatment obtained in this study was higher than in other studies [5,16,19]. The stevioside content in this study was about 62 to 69 mg/g, while in other studies, the content of stevioside after MeJa elicitation was 50 mg/g in liquid WPM medium [16].…”

Section: Discussioncontrasting

confidence: 73%

“…The results showed that 100 μM MeJA treatment for 24 h (treatment B) increased the content of rebaudioside A compared to treatment A. The previous result showed that 100 µM MeJa could increase stevioside and rebaudioside A of Stevia in vitro culture for 4 weeks of elicitation [5]. In other results, 50 μM MeJA elicitation showed a 4-fold increase in stevioside concentration in Stevia leaves [19] and 0.5 mg/L MeJa increased stevioside by 133% and rebaudioside A by 71% in adventitious roots.…”

Section: Discussionmentioning

confidence: 84%

“…Jasmonate, both jasmonic acid and methyl jasmonate, is a cyclopentanone group of compounds that can modulate plant responses widely. They act as an effective elicitor in the production of in vitro culture metabolites and can stimulate the production of secondary metabolites such as terpenoids, flavonoids, alkaloids, and phenylpropanoids [5][6][7]. The objective of this study was to evaluate the effect of MeJA elicitation on plant growth and SG contents (stevioside and rebaudioside A) in Stevia shoot culture cultivated using a mist bioreactor for 14 days.…”

Section: Introductionmentioning

confidence: 99%

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The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

Rahmawati,

Saksyasamita,

Emmanuel

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Steviol glycosides (SG) from Stevia rebaudiana have been used as natural sweeteners to substitute sucrose. SG production naturally needs large biomass. Therefore, it requires large area of cultivation. In vitro cultivation can be used as an alternative to propagate Stevia, as well as for increasing the production of SG. In this experiment, we conducted Stevia propagation in bioreactor and induced SG production through methyl jasmonate (MeJa) elicitation. The objective of this study was to evaluate the effect of MeJA on plant growth and SG contents in Stevia shoot cultivated using mist bioreactor. Elicitation was carried out by MeJa spraying for 30 min every 6 h. In treatment A, we used 10 μM MeJa for 72 h and 100 μM MeJa for 24 h for treatment B. The results showed that treatment A gave higher increase in shoot growth (485%), biomass production (547%), and relative growth rate (0.13) compared to treatment B. There was no significant difference in stevioside content among treatments (A and B) and controls, meanwhile treatment B produced rebaudioside A (0.0113 mg/g) higher than treatment A (0.0062 mg/g). We concluded that treatment A was better in inducing growth and treatment B could be used to increase rebaudioside A.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

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The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

Rahmawati,

Saksyasamita,

Emmanuel

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…An increase in the amount of total isoflavonoid content after elicitation with polysaccharides is often correlated with the up-regulation of key genes related to isoflavonoid biosynthesis [ 60 , 61 ]. Similar evidence of an increase isoflavonoid content was also observed in poly- or oligosaccharides treated in vitro cultures of Pueraria candollei [ 62 ], Nasturtium ofcinale [ 63 ], and Stevia rebaudiana [ 64 ].…”

Section: Discussionsupporting

confidence: 57%

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Kanthaliya

Joshi

Arora

et al. 2023

Plants

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Pueraria tuberosa contains a wide range of bioactive compounds, including polyphenols, alkaloids, and phytosterols, which make it valuable to the pharmaceutical and food industries. Elicitor compounds trigger the defense mechanisms in plants and are widely used to increase the yield of bioactive molecules in in vitro cultures. The present study was conducted to evaluate the effects of different concentrations of biotic elicitors such as yeast extract (YE), pectin (PEC), and alginate (ALG) on growth, antioxidant activity, and metabolite accumulation in in vitro propagated shoots of P. tuberosa. The elicitors applied to shoot cultures of P. tuberosa significantly increased biomass (shoot number, fresh weight, and dry weight), and metabolites such as protein, carbohydrates, chlorophyll, total phenol (TP), and total flavonoid (TF) contents, as well as antioxidant activity compared to untreated control. Biomass, TP, and TF contents, as well as antioxidant activity, were most significant in cultures treated with 100 mg/L PEC. In contrast, chlorophyll, protein, and carbohydrate increased most in cultures treated with 200 mg/L ALG. Application of 100 mg/L of PEC led to the accumulation of high amounts of isoflavonoids including puerarin (220.69 μg/g), daidzin (2935.55 μg/g), genistin (5612 μg/g), daidzein (479.81 μg/g), and biochanin-A (111.511 μg/g) as analyzed by high-performance liquid chromatography (HPLC). Total isoflavonoids content of 100 mg/L PEC treated shoots was obtained as 9359.56 μg/g, 1.68-fold higher than in vitro propagated shoots without elicitors (5573.13 μg/g) and 2.77-fold higher than shoots of the mother plant (3380.17 μg/g). The elicitor concentrations were optimized as 200 mg/L YE, 100 mg/L PEC, and 200 mg/L ALG. Overall, this study showed that the application of different biotic elicitors resulted in better growth, antioxidant activity, and accumulation of metabolites in P. tuberosa, which could lead to obtaining phytopharmaceutical advantages in the future.

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“…Recent studies have shown a higher success rate of 98-100% and increased survival rate of in vitro-propagated plants [45,46]. Additionally, several elicitors and stress environment (salt stress) have proved to be beneficial for increased SvGls synthesis in a fast and cost-effective manner [47][48][49][50]. The possible explanation for this may be the stressinduced changes in the membrane structure, chlorophyll content, and enzyme activity which influence the production of primary and secondary metabolites in the plants.…”

Section: Conventional Propagation and In Vitro Culture Techniquementioning

confidence: 99%

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Rai

Han

2022

Applied Sciences

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Steviol glycosides (SvGls) are plant secondary metabolites belonging to the class diterpenes. SvGls naturally derived from non-calorie sugar have therapeutic properties for diabetics, carcinogens, and anti-microbials. Over the past few years, SvGls have been extensively researched because of their extraordinary sweetness without side effects. SvGls are classified into several types based on the number of sugar bases attached to the steviol. Due to the difference in glycosylation, different SvGls have different sensory properties. One of the main obstacles is the mass production of SvGls. Novel physical and chemical treatments and advanced biotechnological approaches are introduced to increase the production of SvGls. Extraction of high-purity SvGls from plants is difficult, so researchers have manipulated the metabolic pathways of engineered microorganisms for bulk production of SvGls. There are many aspects related to the biochemistry and metabolism of SvGls, but their functional relationship with the S. rebaudiana is not well understood. Thus there is a need for in-depth research in this area. This review provides the readers with an overview of the research gaps and possible promising methodologies that can be utilized in the future. To trigger more research, this review encompasses the recent developments in SvGls production and marketing.

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Elicitor-induced in vitro shoot multiplication and steviol glycosides production in Stevia rebaudiana

Cited by 22 publications

References 54 publications

The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

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