Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L

Bach, M.; Schnitzler, Jörg‐Peter; Seitz, Hanns Ulrich

doi:10.1104/pp.103.2.407

Cited by 72 publications

(40 citation statements)

References 21 publications

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“…The dependence of an oxidative burst on cell wall peroxidases was initially reported in carrot suspension cells (Bach et al, 1993). ROS formation dependent on an apoplastic peroxidase was later confirmed in other plants (bean, Arabidopsis, pepper, lettuce, cotton) (Bestwick et al, 1998;Bindschedler et al, 2006;Bolwell, 1999;Choi et al, 2007;Martinez et al, 1998).…”

Section: Ros and Resistance To Fungal Pathogens Of Leavesmentioning

confidence: 80%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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Section: Ros and Resistance To Fungal Pathogens Of Leavesmentioning

confidence: 80%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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“…The natural physiological substrates used by these peroxidases to generate ROS have not yet been identified. Peroxidase-dependent oxidative bursts have been described in Arabidopsis Davies et al, 2006), Daucus carota (Bach et al, 1993), French bean (Phaseolus vulgaris; Bolwell, 1995Bolwell, , 1999Bolwell et al, 2001), Capsicum annum (Choi et al, 2007), and Lactuca sativa (Bestwick et al, 1997). Importantly, Arabidopsis plants compromised for the expression of at least two cell wall peroxidase-encoding genes, PRX33 and PRX34, exhibited dramatically enhanced susceptibility to a variety of fungal and bacterial pathogens Daudi et al, 2012).…”

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confidence: 99%

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

O’Brien

Daudi²,

Finch³

et al. 2012

Plant Physiology

189

152

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Perception by plants of so-called microbe-associated molecular patterns (MAMPs) such as bacterial flagellin, referred to as pattern-triggered immunity, triggers a rapid transient accumulation of reactive oxygen species (ROS). We previously identified two cell wall peroxidases, PRX33 and PRX34, involved in apoplastic hydrogen peroxide (H 2 O 2 ) production in Arabidopsis (Arabidopsis thaliana). Here, we describe the generation of Arabidopsis tissue culture lines in which the expression of PRX33 and PRX34 is knocked down by antisense expression of a heterologous French bean (Phaseolus vulgaris) peroxidase cDNA construct. Using these tissue culture lines and two inhibitors of ROS generation, azide and diphenylene iodonium, we found that perxoxidases generate about half of the H 2 O 2 that accumulated in response to MAMP treatment and that NADPH oxidases and other sources such as mitochondria account for the remainder of the ROS. Knockdown of PRX33/PRX34 resulted in decreased expression of several MAMP-elicited genes, including MYB51, CYP79B2, and CYP81F2. Similarly, proteomic analysis showed that knockdown of PRX33/PRX34 led to the depletion of various MAMP-elicited defense-related proteins, including the two cysteine-rich peptides PDF2.2 and PDF2.3. Knockdown of PRX33/PRX34 also led to changes in the cell wall proteome, including increases in enzymes involved in cell wall remodeling, which may reflect enhanced cell wall expansion as a consequence of reduced H 2 O 2 -mediated cell wall cross-linking. Comparative metabolite profiling of a CaCl 2 extract of the PRX33/PRX34 knockdown lines showed significant changes in amino acids, aldehydes, and keto acids but not fatty acids and sugars. Overall, these data suggest that PRX33/PRX34-generated ROS production is involved in the orchestration of patterntriggered immunity in tissue culture cells.

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“…In tobacco, soybean, carrot, and parsley, this requirement correlates with rapid elicitorstimulated Ca 2ϩ influx (11,(18)(19)(20), suggesting that Ca 2ϩ is an important element in elicitor-mediated signal transduction, as it is in many other signaling processes (21,22). This and other early responses to elicitor treatment, such as various ion fluxes across the plasma membrane, synthesis of ROS, and phosphorylation and dephosphorylation of proteins, have frequently been discussed as putative components of signal transduction chain(s) leading to defense gene activation and͞or hypersensitive cell death (4,(15)(16)(17)(23)(24)(25)(26).…”

mentioning

confidence: 99%

Elicitor-stimulated ion fluxes and O ₂ ⁻ from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley

Jabs¹,

Tschöpe²,

Colling³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

542

391

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Fungal elicitor stimulates a multicomponent defense response in cultured parsley cells (Petroselinum crispum). Early elements of this receptor-mediated response are ion f luxes across the plasma membrane and the production of reactive oxygen species (ROS), sequentially followed by defense gene activation and phytoalexin accumulation. Omission of Ca 2؉ from the culture medium or inhibition of elicitor-stimulated ion f luxes by ion channel blockers prevented the latter three reactions, all of which were triggered in the absence of elicitor by amphotericin B-induced ion f luxes. Inhibition of elicitor-stimulated ROS production using diphenylene iodonium blocked defense gene activation and phytoalexin accumulation. O 2 ؊ but not H 2 O 2 stimulated phytoalexin accumulation, without inducing proton f luxes. These results demonstrate a causal relationship between early and late reactions of parsley cells to the elicitor and indicate a sequence of signaling events from receptor-mediated activation of ion channels via ROS production and defense gene activation to phytoalexin synthesis. Within this sequence, O 2 ؊ rather than H 2 O 2 appears to trigger the subsequent reactions.

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Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L

Cited by 72 publications

References 21 publications

Reactive oxygen species and plant resistance to fungal pathogens

Reactive oxygen species and plant resistance to fungal pathogens

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

Elicitor-stimulated ion fluxes and O ₂ ⁻ from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley

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Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L

Cited by 72 publications

References 21 publications

Reactive oxygen species and plant resistance to fungal pathogens

Reactive oxygen species and plant resistance to fungal pathogens

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

Elicitor-stimulated ion fluxes and O 2 − from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley

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Elicitor-stimulated ion fluxes and O ₂ ⁻ from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley