Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L.

Ali, Mohammad; Abbasi, Bilal Haider; Ali, Gul Shad

doi:10.1007/s11240-014-0666-2

Cited by 66 publications

(36 citation statements)

References 30 publications

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“…Our results were consistent with those of previous studies; total phenolic and flavonoid contents were increased by JA and SA in the cell suspension culture of P. ginseng [38]. JA and SA enhanced phenolic and flavonoid accumulation in the suspension cultures of A. absinthium [39]. Cell suspension cultures enhanced the production of total phenolic and flavonoid contents in A. absinthium [23].…”

Section: Resultssupporting

confidence: 93%

“…Figure 4f shows that the percentage of metal scavenging capacity exhibited by the JA (98.05%), SA (89.15%), and PGR culture cells (81.30%) was high when compared with the control (69.15%) cells. JA- and SA-elicited suspension cells increased the antioxidant activity of P. ginseng [38] and A. absinthium [39], which is consistent with our results.…”

Section: Resultssupporting

confidence: 91%

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Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

Thiruvengadam

Rekha

Rajakumar

et al. 2016

IJMS

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Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of Polygonum multiflorum. We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the production of phytochemicals and biomass accumulation in a cell suspension culture of P. multiflorum. The medium containing Murashige and Skoog (MS) salts and 4% sucrose supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L thidiazuron, and 100 µM l-glutamine at 28 days of cell suspension culture was suitable for biomass accumulation and AQ production. Maximum biomass accumulation (12.5 and 12.35 g fresh mass (FM); 3 and 2.93 g dry mass (DM)) and AQ production (emodin 295.20 and 282 mg/g DM; physcion 421.55 and 410.25 mg/g DM) were observed using 100 µM JA and SA, respectively. JA- and SA-elicited cell cultures showed several-fold higher biomass accumulation and AQ production than the control cell cultures. Furthermore, the cell suspension cultures effectively produced 23 phenolic compounds, such as flavonols and hydroxycinnamic and hydroxybenzoic acid derivatives. PGR-, JA-, and SA-elicited cell cultures produced a higher amount of AQs and phenolic compounds. Because of these metabolic changes, the antioxidant, antimicrobial, and anticancer activities were high in the PGR-, JA-, and SA-elicited cell cultures. The results showed that the elicitors (JA and SA) induced the enhancement of biomass accumulation and phytochemical (AQs and phenolic compounds) production as well as biological activities in the cell suspension cultures of P. multiflorum. This optimized protocol can be developed for large-scale biomass accumulation and production of phytochemicals (AQs and phenolic compounds) from cell suspension cultures, and the phytochemicals can be used for various biological activities.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 91%

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

Thiruvengadam

Rekha

Rajakumar

et al. 2016

IJMS

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“…This study resulted in a significant increase in the cell number and dry weight in the MeJA-and SA-treated cell suspensions when compared to the control culture. These results are in agreement with those of Ali et al (2015), who showed that a 2.6-fold, 2.9-fold, and 3-fold increase in dry biomass was observed in Artemisia absinthium cultures treated with 0.5, 1.0, and 2.0 mg L -1 MeJA, respectively. However, Ali et al (2007) reported different results, in which the fresh and dry weights decreased significantly after 9 days of exposure to SA and MeJA in Panax ginseng root suspension culture.…”

Section: Discussionsupporting

confidence: 93%

Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow(Achilleagypsicola) species

Açıkgöz¹,

Kara²,

Aygün³

et al. 2019

Turk J Agric For

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Elicitors have been widely used as biotic and abiotic stimulants in triggering the production of secondary metabolites in plant cell culture. The present study aimed to enhance the production of camphor and phenolic compounds and cell growth using methyl jasmonate (MeJA) and salicylic acid (SA) in the cell suspension culture of Turkish endemic species Achillea gypsicola. Various concentrations (0, 10, 50, and 100 µM) of MeJA and SA were applied to 8-day-old cell cultures. The camphor and phenolic compound contents were determined using a headspace gas chromatographic-mass spectrometer device and spectrophotometer. Increasing doses of MeJA and SA significantly enhanced the accumulation of the camphor and phenolic compounds in general. The highest amount of camphor accumulation occurred in cells treated with 100 µM MeJA (0.3449 µg/g) and 50 µM SA (0.3816 µg/g). Increasing concentrations of MeJA resulted in a significant decrease in the total anthocyanin when compared to the initial culture. The present study showed that MeJA and SA could effectively be used as potent elicitors to enhance the production of camphor and phenolic compounds, along with cell growth, in cell suspension cultures of the endemic Turkish yarrow species Achillea gypsicola.

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“…Cells were harvested by filtration through filter paper (Sartorius ™ Quantitative Grade 292 Filter Papers; Fisher Scientific, USA), rinsed with distilled water and blotted to remove excess water. The cells were then oven dried at 60°C for 24 h to determine the dry weight (DW) [42]. Triplicate flasks were used in this experiment.…”

Section: Methodsmentioning

confidence: 99%

Successes of artemisinin elicitation in low-artemisinin producing Artemisia annua cell cultures constrained by repression of biosynthetic genes

Yee

Ping

2019

Preprint

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10The sesquiterpene phytolactone derived from Artemisia annua, artemisinin is 11 associated with a variety of novel biological properties, such as immunoregulatory and 12 anticancer effects, and therapeutic applications, apart from its main function as an 13 4 57 Naturally, artemisinin biosynthesis occurs in glandular secretory trichomes that are 58 present on foliage, stems and inflorescences of the A. annua plant [15, 16]. Due to its 59 compartmentalized synthesis and influence by physiological, seasonal and environmental 60 factors, artemisinin concentration is highly variable with reported values of 0.1 -10 mg g -1 61 dry weight (DW) [11, 17]. With the biosynthetic pathway of artemisinin formation fully 62 elucidated over the past decade, it was learnt that within the species A. annua two contrasting 63 chemotypes can be distinguished, which are characterized by the differing contents of 64 artemisinin and its precursor (Fig 1). While both possess artemisinin, the high-artemisinin 65 producing (HAP) chemotype contains relatively higher levels of dihydroartemisinic acid 66 (DHAA) and artemisinin, and the low-artemisinin producing (LAP) chemotype has higher 67 contents of artemisinic acid and arteannuin B (a non-antimalarial product) [18, 19]. Despite 68 the difference in biochemical phenotype which is attributed to the differential expression of 69 one artemisinin biosynthesis specific gene artemisinic aldehyde Δ11(13) reductase (DBR2), 70 both chemotypes undergo similar spontaneous photooxidation reactions to produce 71 artemisinin or arteannuin B from its direct precursors [20-23]. 72 Fig 1. Schematic representation of artemisinin biosynthesis. 73 Synthesis occurs in the glandular trichome secretory cells of A. annua and sequestration of 74 artemisinin takes place in the subcuticular space. The two branching pathways leading to 75 artemisinin and arteannuin B of the artemisinin biosynthetic pathway (dashed line box) are 76 depicted and the final non-enzymatic photo-oxidation reaction is shown in red. MEP, 77 methylerythritol 4-phosphate; MVA, mevalonate; GST, glandular secretory trichome; ADS, 78 amorpha-4,11-diene synthase; CYP71AV1, cytochrome P450 monooxygenase; DBR2, 79 artemisinic aldehyde Δ11(13) reductase; ALDH1, aldehyde dehydrogenase 1. 80 As a secondary metabolite used mainly in defence mechanisms, production of 81 artemisinin would naturally be induced and enhanced under environmental stresses. Under 5 82 such adverse conditions the elevation of secondary oxidative stress, which is a consequence 83 of primary biotic/abiotic stress, is in a direct synergistic relationship with artemisinin 84 production [24-27]. On such basis, the biotechnological method elicitation which mimics the 85 natural induction of plant stress is the method of choice to enhance the production of 86 artemisinin. The positive effects of UV-B and DMSO on the production of artemisinin had 87 been verified by previous studies on A. annua seedlings [28, 29], in vitro propagated plantlets 88 [30] and shoot cultures [31]. Former st...

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Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L.

Cited by 66 publications

References 30 publications

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow(Achilleagypsicola) species

Successes of artemisinin elicitation in low-artemisinin producing Artemisia annua cell cultures constrained by repression of biosynthetic genes

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