Elevation of miR-21, through targeting MKK3, may be involved in ischemia pretreatment protection from ischemia–reperfusion induced kidney injury

Li, Zhihui; Deng, Xu; Kang, Zhuang; Wang, Ying; Xia, Tuanhong; Ding, Niu; Yin, Yan

doi:10.1007/s40620-015-0217-x

Cited by 35 publications

(31 citation statements)

References 31 publications

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“…In the other approach, several studies have suggested a reno-protective role for miR-21 and believe that upregulation of miR-21 plays a physiological role in protecting cells from acute injury by antiapoptotic effects and silencing metabolic functions. However, in chronic conditions, long-term elevation of miR-21 is detrimental and promotes cells toward infl ammation and fi brogenesis (39)(40)(41)(42)(43).…”

Section: Discussionmentioning

confidence: 99%

Early detection of cardiac surgery‑associated acute kidney injury by microRNA-21

Arvin¹,

Samimagham²,

Montazerghaem³

et al. 2018

BLL

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We tested the hypothesis whether microRNA-21 (miR-21) can detect CSA-AKI earlier than serum creatinine (sCr). A total of 103 patients scheduled to undergo cardiac surgery. CSA-AKI was defined as sCr > 0.3 mg/dl 24 h after surgery. The patients were divided into two groups according to whether or not developing AKI after surgery. Serum and urinary miR-21 were measured prior to, and 6, 12 and 24 h after surgery. Baseline serum and urinary levels of miR-21 in AKI group were lower than in non-AKI group. Moreover, the levels of miR-21 were significantly lower 6 h after surgery for serum, and 6 and 12 h after surgery for urine samples than those before surgery in AKI group. Area under the curve (AUC) of the receiver operating characteristic (ROC) values were 0.81 (95% CI: 0.65-0.97) for serum miR-21 (6 h after surgery), 0.90 (95% CI: 0.79-0.99) for urine (6 h after surgery), and 0.86 (95% CI: 0.71-0.98) for urine (12 h after surgery). While both postoperative serum and urinary miR-21 levels can predict AKI development, urinary miR-21 especially 6 h after surgery is a more reliable marker than serum miR-21 for detection of established CSA-AKI (Tab. 1, Fig. 3, Ref. 43).

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Section: Discussionmentioning

confidence: 99%

Early detection of cardiac surgery‑associated acute kidney injury by microRNA-21

Arvin¹,

Samimagham²,

Montazerghaem³

et al. 2018

BLL

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“…Our data respectively showed the interactive network of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA, which play a regulatory role as observed with mmu-miR-132-3p, mmu-miR-17-5p, mmu-miR-21a-5p, mmu-miR-21a-3p, mmu-miR-20a-5p, mmu-miR-93-5p, mmu-miR-185-5p and mmu-miR-874-3p. It is worth mentioning that miR-21, miR-223-5p, miR-125b and so on proved to be involved in IR-induced AKI, did not appear in our data [ 53 - 55 ]. The reason is that all ncRNA sequencing analysized were based on the lncRNA library, not built separate library of miRNA.…”

Section: Discussionmentioning

confidence: 74%

Systematic analysis of the expression profile of non-coding RNAs involved in ischemia/reperfusion-induced acute kidney injury in mice using RNA sequencing

2017

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Acute kidney injury (AKI) is a common and serious disease characterized by a rapid decline in renal function and has an unacceptably high mortality rate with no effective treatment beyond supportive care. AKI can be induced by many factors such as ischemia/reperfusion (IR), sepsis, and drug-induced nephrotoxicity. However, the molecular mechanisms of AKI are poorly understood. A non-coding RNA (ncRNA) is a RNA molecule that is not translated into a protein. NcRNAs play multiple roles in cellular processes, and mutations or imbalances of these molecules within the body can cause a variety of diseases. Although growing evidence has supported the key role of ncRNAs in AKI, the specific mechanism remains largely unknown. In this study, the second-generation gene sequencing was performed to investigate the expression patterns of ncRNAs, including microRNA (miRNA), long non-coding RNAs, and circular RNAs, in the kidneys of mice subjected to IR-induced AKI. This information will contribute to future research of the mechanism of ncRNAs in the pathogenesis of AKI and facilitate the identification of novel therapeutic targets of ncRNAs.

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“…BUN and Scr in pediatric patients with AKI were decreased in parallel during hospitalization in all age groups [26]. BUN and SCr are both considered speci c markers to measure kidney function and pathological injuries after I/R [27]. SOD could limits oxidative stress and renal I/R injury and is considered as the most relevant molecule against I/R-induced changes [28].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA TUG1/microRNA-29/PTEN axis in ischemia-reperfusion in a rat model

Xu¹,

Huang²,

Lin³

et al. 2020

Preprint

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Objective: Long non-coding RNA (lncRNA) taurine upregulated gene 1 (TUG1) is increased under ischemia. This study intended to identify the potential competing endogenous RNA network involving TUG1 in renal ischemia-reperfusion (I/R).Methods: A rat model of acute renal injury induced by I/R was established, and the differentially expressed genes were analyzed by microarray. The levels of blood urea nitrogen (BUN), serum creatine (SCr), methylenedioxyphetamine (MDA) and superoxide dismutase (SOD) in serum of rats were measured. HE staining evaluated the pathological damage of renal tissues, western blot analysis detect the levels of apoptosis- and autophagy-related proteins, immunofluorescence staining detected LC3 fluorescence intensity, and transmission electron microscope observed autophagosomes. Pull-down assay and dual luciferase reporter gene assay were used to verify the targeting relationship among TUG1, miR-29 and PTEN. The effects of TUG1 on biological behaviors of renal tubular cells were evaluated by simulating the acute renal injury induced by I/R in vitro.Results: In vivo, the levels of BUN, SCr and MDA in serum of I/R-treated rats were increased, SOD level and autophagosomes were reduced, tubule epithelial cells were necrotic, and TUG1 was upregulated in renal tissues of I/R-treated rats, which were reversed by TUG1 knockdown. Autophagy inhibition attenuated the protective effect of TUG1 knockdown on I/R-treated rats. TUG1 could competitively bind to miR-29 to promote PTEN expression. In vitro, low expression of TUG1 promoted proliferation and autophagy of renal tubular cells and inhibited apoptosis.Conclusion: TUG1 knockdown promotes autophagy and improves acute renal injury in I/R-treated rats by binding to miR-29 to silence PTEN.

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Elevation of miR-21, through targeting MKK3, may be involved in ischemia pretreatment protection from ischemia–reperfusion induced kidney injury

Abstract: miR-21 targets MKK3 in vivo and in vitro, inhibiting the downstream factors IL-6 and TNF-α. Therefore, miR-21 might be involved in protection of IPC against IR of the kidney.

Cited by 35 publications

References 31 publications

Early detection of cardiac surgery‑associated acute kidney injury by microRNA-21

Early detection of cardiac surgery‑associated acute kidney injury by microRNA-21

Systematic analysis of the expression profile of non-coding RNAs involved in ischemia/reperfusion-induced acute kidney injury in mice using RNA sequencing

Long non-coding RNA TUG1/microRNA-29/PTEN axis in ischemia-reperfusion in a rat model

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