Elevated urinary 8-oxo-7,8-dihydro-2&amp;#39;-deoxyguanosine and serum uric acid are associated with progression and are prognostic factors of colorectal cancer

Mao, Lingna; Guo, Cheng; Zheng, Shu

doi:10.2147/ott.s175112

Cited by 15 publications

(10 citation statements)

References 33 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Healthy controls Patients Methods Ref. ‘DNA’ oxidation 3.68 (n = 61) 4.58 (n = 57; unipolar and bipolar disorders) LC-MS/MS [ 308 ] 8-oxodG (ng/mg creatinine) 2.53 (n = 70) 3.46 (n = 148; bipolar I disorder) UPLC-MS/MS [ 309 ] 3.90 (n = 128) 4.35 (n = 87; COPD); 14.6 (n = 100; patients with mechanical ventilation) LC-MS/MS [ 241 ] 4.13 (n = 16) 5.23 (n = 46; renal disease) LC-MS/MS [ 310 ] 2.80 (n = 73) 4.71 (n = 60; breast cancer) UPLC-MS/MS [ 311 ] - a 5.41 (n = 12; colorectal cancer) UPLC-MS/MS [ 312 ] – 6.76 (n = 508; cardiovascular disease) UPLC-MS/MS [ 313 ] – 4.62 (n = 2721; type 2 diabetes) UPLC-MS/MS [ 314 ] – 5.27 (n = 609; type 2 diabetes) UPLC-MS/MS [ 109 ] 2.03 (n = 60) – UPLC-MS/MS [ 315 ] 7.83 (n = 132) …”

Section: Nucleic Acid Oxidation In Human Health and Diseasementioning

confidence: 99%

Biomarkers of nucleic acid oxidation – A summary state-of-the-art

Chao

Evans

et al. 2021

Redox Biology

View full text Add to dashboard Cite

Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the other nucleic acids, RNA and the (2′-deoxy-)ribonucleotide pools, and evidence is growing that these too may have an important role in disease. LC-MS/MS has the ability to provide absolute quantification of specific biomarkers, such as 8-oxo-7,8-dihydro-2′-deoxyGuo (8-oxodG), in both nuclear and mitochondrial DNA, and 8-oxoGuo in RNA. However, significant quantities of tissue are needed, limiting its use in human biomonitoring studies. In contrast, the comet assay requires much less material, and as little as 5 μL of blood may be used, offering a minimally invasive means of assessing oxidative stress in vivo , but this is restricted to nuclear DNA damage only. Urine is an ideal matrix in which to non-invasively study nucleic acid-derived biomarkers of oxidative stress, and considerable progress has been made towards robustly validating these measurements, not least through the efforts of the European Standards Committee on Urinary (DNA) Lesion Analysis. For urine, LC-MS/MS is considered the gold standard approach, and although there have been improvements to the ELISA methodology, this is largely limited to 8-oxodG. Emerging DNA adductomics approaches, which either comprehensively assess the totality of adducts in DNA, or map DNA damage across the nuclear and mitochondrial genomes, offer the potential to considerably advance our understanding of the mechanistic role of oxidatively damaged nucleic acids in disease.

show abstract

Section: Nucleic Acid Oxidation In Human Health and Diseasementioning

confidence: 99%

Biomarkers of nucleic acid oxidation – A summary state-of-the-art

Chao

Evans

et al. 2021

Redox Biology

View full text Add to dashboard Cite

show abstract

“…Evidence has shown that preoperative SUA is an independent prognostic predictor in esophageal squamous cell carcinoma patients who undergo R0 esophagectomy, and patients with a higher SUA level might have significantly shorter 1, 3, and 5 year survival times than patients with a relatively low SUA level [22]. For colorectal cancer (CRC), it was found that SUA levels gradually increased from stage I to stage IV, suggesting that the SUA level reflected the severity of CRC and may help to evaluate the therapy effect as well as the prognoses of CRC patients [14]. In addition, an elevated serum level of uric acid was shown to be a significant prognostic marker for lymphatic metastasis in patients with colon cancer [23].…”

Section: Uric Acid As a Risk Factor In Various Cancersmentioning

confidence: 99%

“…In addition, serum uric acid in very high concentrations may trigger inflammatory stress, and it may also have intracellular pro-oxidative activity 4 , 13 . It is well-known that a pro-oxidant environment confers a growth advantage to tumor cells, and also influences carcinogenic potential by stimulating specific signaling cascades that regulate cell growth and apoptosis 14 .…”

Section: Introductionmentioning

confidence: 99%

Friend or Foe? An Unrecognized Role of Uric Acid in Cancer Development and the Potential Anticancer Effects of Uric Acid-lowering Drugs

Mi¹,

Gong²,

Sui³

2020

J. Cancer

View full text Add to dashboard Cite

In recent years, metabolic syndrome (Mets) has been a hot topic among medical scientists. Mets has an intimate relationship with the incidence and development of various cancers. As a contributory factor of Mets, hyperuricemia actually plays an inseparable role in the formation of various metabolic disorders. Although uric acid is classically considered an antioxidant with beneficial effects, mounting evidence indicates that a high serum uric acid (SUA) level may serve as a pro-oxidant to generate inflammatory reactions and oxidative stress. In this review, we describe the unrecognized role of hyperuricemia in cancer development and summarize major mechanisms linking uric acid to carcinogenesis. Furthermore, we also discuss the potential mechanism of liver metastasis of cancer and list some types of uric acid-lowering agents, which may exert anticancer effects.

show abstract

“…In 2016, Guo et al. developed an offline SPE‐UPLC–MS/MS method for the quantification of 8‐OHdG in urine samples and it was successfully applied to compare the urinary levels in patients with colorectal cancer with those of healthy controls, both in 2016 [103] and in 2018 [104]. Recently, the same research group developed an analytical method for the measurement of 8‐OHG in human urine, and again, this method was successfully applied for the determination of the RNA OS biomarker in the urine of both patients with colorectal cancer and healthy volunteers [105].…”

Section: Strategies To Quantify Biomarkers In Human Urine (2015–2020)mentioning

confidence: 99%

Recent progress in the LC–MS/MS analysis of oxidative stress biomarkers

et al. 2020

View full text Add to dashboard Cite

The presence of a dynamic and balanced equilibrium between the production of reactive oxygen (ROS) and nitrogen (RNS) species and the in‐house antioxidant defense mechanisms is characteristic for a healthy body. During oxidative stress (OS), this balance is switched to increased production of ROS and RNS, exceeding the capacity of physiological antioxidant systems. This can cause damage to biological molecules, leading to loss of function and even cell death. Nowadays, there is increasing scientific and clinical interest in OS and the associated parameters to measure the degree of OS in biofluids. An increasing number of reports using LC–MS/MS methods for the analysis of OS biomarkers can be found. Since bioanalysis is usually complicated by matrix effects, various types of cleanup procedures are used to effectively separate the biomarkers from the matrix. This is an essential part of the analysis to prepare a reproducible and homogenous solution suitable for injection onto the column. The present review gives a summary of the chromatographic methods used for the determination of OS biomarkers in both urine and plasma, serum, and whole blood samples. The first part mainly describes the biological background of the different OS biomarkers, while the second part reports examples of chromatographic methods for the analysis of different metabolites connected with OS in biofluids, covering a period from 2015 till early 2020. The selected examples mainly include LC–MS/MS methods for isoprostanes, oxidized proteins, oxidized lipoproteins, and DNA/RNA biomarkers. The last part explains the clinical relevance of this review.

show abstract

Elevated urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine and serum uric acid are associated with progression and are prognostic factors of colorectal cancer

Cited by 15 publications

References 33 publications

Biomarkers of nucleic acid oxidation – A summary state-of-the-art

Biomarkers of nucleic acid oxidation – A summary state-of-the-art

Friend or Foe? An Unrecognized Role of Uric Acid in Cancer Development and the Potential Anticancer Effects of Uric Acid-lowering Drugs

Recent progress in the LC–MS/MS analysis of oxidative stress biomarkers

Contact Info

Product

Resources

About