Elevated TNF-α and LDH without parathormone disturbance is associated with diffuse osteolytic lesions in leukemic transformation of myelofibrosis

Jurišić, Vladimir; Terzić, Tatjana; Pavlović, Sonja; Čolović, Nataša; Čolović, Milica

doi:10.1016/j.prp.2007.09.001

Cited by 20 publications

(24 citation statements)

References 14 publications

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“…The aim was to standardize the values in relation to controls under our laboratory conditions. Our and previous reports showed that in cancer patients the total ALP enzyme activity was around three-fold higher than the activity in healthy people of the same age (9,12). ALP isoenzymes were identified by heat inactivation, which is, besides electrophoresis (10,11), the most frequently applied method.…”

Section: Discussionmentioning

confidence: 79%

Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis

et al. 2013

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Background: Alkaline phosphatase is a glycoprotein that catalyzes two kinds of chemical reactions: hydrolysis of phosphorus ester breaking P-O bonds and phospho-transfer reactions in which phosphoric group is transferred to an acceptor molecule. In the human body, ALP exists in multiple molecular forms whose heterogeneity is partly due to genetic factors and partly to posttranslational modifications. The aim was to evaluate a total ALP activity and its isoforms in cancer patients with bone and liver metastasis in comparison to healthy controls. Methods: Human serum was collected from 20 healthy individuals, and 20 cancer patients with bone and liver metastases, with metastases confirmed by ultrasound, computerized tomography and a radiology scan. Determination of ALP was done by the endpoint spectrophotometric method. Isoenzymes were determined by heat inactivation method. Results: In cancer patients, the total ALP activity was significantly higher (p< 0.05) compared to healthy controls. In the sera of cancer patients with liver metastases, the remaining ALP activity was two-fold higher in comparison to bone metastases. Conclusion: Determination of ALP isoenzymes is important but a correct clinical interpretation in the context of other analyses is vital for a proper diagnosis of a disease. [Projekat Ministarstva nauke Republike Srbije, br.175056

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Section: Discussionmentioning

confidence: 79%

Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis

et al. 2013

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“…Osteolytic lesions could also be a consequence of leukemic bone infiltration or focal bone destruction by tumor necrosis factor-alfa locally released by leukemic cells as a result of leukemic transformation of PMF [10]. While osteosclerosis therapy, even though not codified, is mainly based on radiation therapy [11], no data are now available concerning the optimal therapeutic approach to osteolytic lesions.…”

Section: Discussionmentioning

confidence: 99%

Osteolytic Lesions in Primary Myelofibrosis and Effect of Ruxolitinib Therapy: Report of a Case and Literature Review

et al. 2018

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Here, we report the case of a young female affected by primary myelofibrosis (PMF) who developed an osteolytic lesion of the humerus during the follow-up, and the possible efficacy of ruxolitinib in controlling this rare event. After 26 years of follow-up, the patient reported onset of acute pain at the proximal region of the left upper limb. An X-ray revealed an osteolytic bone lesion in the proximal third of the humeral shaft, which was then confirmed by magnetic resonance imaging. A biopsy of the lytic lesion was done, revealing hypercellular bone marrow with hyperplastic granulopoiesis associated with megakaryocytic proliferation and atypia, accompanied by a diffuse and dense increase in reticulin fibrosis with extensive intersections and coarse bundles of thick fibers, consistent with a grade 3 collagen fibrosis. No new therapeutic intervention was initially required; however, 2 years later, the patient reported symptomatic splenomegaly and drenching night sweats, so ruxolitinib therapy was started. By week 8, the patient had near resolution of constitutional symptoms and a reduction of > 50% of the spleen size that normalized by 6 months; in addition, a repeat bone marrow biopsy showed a decrease in reticulin fibrosis grade. Interestingly, after 9 months of ruxolitinib therapy, further magnetic resonance imaging of the left upper limb showed the absence of bone lytic lesions and a substantial normalization of the bone tissue. In conclusion, with the present case report, we confirm ruxolitinib efficacy in reducing bone marrow fibrosis grade and assume its possible role in the resolution of osteolytic lesions in PMF. Obviously, further studies with a greater number of patients are needed to document the exact frequency of these unusual findings and the possible role of ruxolitinib in their treatment.

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“…Furthermore, supplementation of IL-4 to synovial fibroblasts can modulate gene expression profile, resulting with highly modulated fibroblast function [29]. On the other hand, previous studies confirmed the significant role of TNF-α in bone lesions [30, 31], but these requires further analysis.…”

Section: Discussionmentioning

confidence: 99%

Relation between bone mineral density and IL-17 serum levels in Serbian patients with early Rheumatoid arthritis

et al. 2014

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AbstractRheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial inflammation and destruction of joint cartilage and bone. Different cytokines play important role in the processes that cause articular destruction and extra-articular manifestations in RA. The contribution of cytokines representing the Th1 (INF-γ), Th2 (IL-4) and IL-17A to the pathogenesis of early RA and bone mineral density (BMD) loss in still poorly understood. Serum samples of 38 early RA patients were evaluated for erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), C-reactive protein (CRP), anti-cyclic citrullinated peptide antibodies (anti-CCP) and for the tested cytokines (IL-17A, IL-4 and INF-γ). BMD was evaluated by dualenergyX-ray absorptiometry (DXA). Disease activity score (DAS28) calculation was assessed for all patients. Control serum samples were obtained from 34 healthy volunteers. The levels of tested cytokines were significantly higher (IL-17A, p<0.001; INF-γ, P<0.001; IL-4, P<0.01) in patients with early RA, compared to the healthy controls. In early RA patients, strong correlation of serum IL-17A was found with DAS28, ESR and CRP. Also, a significant negative correlation was found between serum INF-γ levels and the DAS28 score. Significantly positive correlation of BMD values and CRP, DAS28 IL-17A were also demonstrated. DXA analysis revealed that the most common site for osteoporosis was the lumbar spine followed by the femoral neck. BMD values significantly correlated with CRP, DAS28 score and IL-17A serum levels. The mean serum IL-17A levels, in patients with early RA, corresponded with disease activity, severity and BMD loss, indicating the potential usefulness of serum IL-17A in defining the disease activity and bone remodeling.

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Elevated TNF-α and LDH without parathormone disturbance is associated with diffuse osteolytic lesions in leukemic transformation of myelofibrosis

Cited by 20 publications

References 14 publications

Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis

Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis

Osteolytic Lesions in Primary Myelofibrosis and Effect of Ruxolitinib Therapy: Report of a Case and Literature Review

Relation between bone mineral density and IL-17 serum levels in Serbian patients with early Rheumatoid arthritis

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