Elevated messenger RNA expression and plasma protein levels of osteopontin and matrix metalloproteinase types 2 and 9 in patients with ascending aortic aneurysms

Huusko, Tuija; Salonurmi, Tuire; Taskinen, Panu; Liinamaa, Johanna; Juvonen, Tatu; Pääkkö, Paavo; Savolainen, Markku J.; Kakko, Sakari

doi:10.1016/j.jtcvs.2012.04.008

Cited by 33 publications

(33 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No significant differences were observed between specimens from patients with IAAAs and those with Marfan syndrome [28,29]. In this study, we confirmed that MMP-2 expression was significantly increased at both transcriptional and translational levels in aortic specimens of IAAA patients compared with in normal aortas, which is consistent with previous studies [8,9,30]. In addition, the expressions of MMP-1, MMP-3, MMP-9, and MMP-14 were markedly increased in IAAAs [6,9].…”

Section: Discussionsupporting

confidence: 92%

Angiotensin II induces an increase in MMP-2 expression in idiopathic ascending aortic aneurysm via AT1 receptor and JNK pathway

Wang

Chang

Qian

et al. 2015

ABBS

View full text Add to dashboard Cite

The cellular and molecular mechanisms responsible for human idiopathic ascending aortic aneurysm (IAAA) remain unknown. Matrix metalloproteinase-2 (MMP-2) is a key enzyme for the degradation of extracellular matrix in aneurysmal walls. The aim of this study was to elucidate the role of the angiotensin II (Ang II) pathway in MMP-2 induction in IAAA aortic walls. Quantitative polymerase chain reaction and western blot analysis were used to compare the MMP-2 mRNA and protein levels in ascending aortic specimens with those in IAAA patients (n = 10) and heart transplant donors (n = 5) without any aortopathy. It was found that MMP-2 expression was significantly increased, which was associated with elastic lamellae disruption in IAAA walls. Additionally, the expression levels of angiotensinogen (AGT) and Ang II in the ascending aortic tissues from individuals with and without IAAAs were detected by western blot analysis and radioimmunoassay, respectively. The results demonstrated that the expressions of AGT and Ang II protein were significantly increased in the ascending aortic tissues of IAAA patients. Furthermore, whether Ang II induces MMP-2 expression was investigated using human IAAA walls ex vivo culture. It was found that exogenous Ang II increased the MMP-2 expression in a dose-dependent manner, which was completely inhibited by the Ang II type 1 receptor (AT1R) inhibitor candesartan and was mediated by c-Jun N-terminal kinase (JNK) activation. Taken together, these results indicate that Ang II can induce an increase of MMP-2 expression via AT1R and JNK in ex vivo cultured IAAA aortic walls, and suggest that angiotensin receptor blocker (ARB) drugs and JNK inhibitors have the potential in the prevention or treatment of IAAAs.

show abstract

Section: Discussionsupporting

confidence: 92%

Angiotensin II induces an increase in MMP-2 expression in idiopathic ascending aortic aneurysm via AT1 receptor and JNK pathway

Wang

Chang

Qian

et al. 2015

ABBS

View full text Add to dashboard Cite

show abstract

“…It has been recently demonstrated that S100A4 in its extracellular form induces expression and secretion of osteopontin (OPN) in osteosarcoma cell lines [33]. OPN, a multifunctional extracellular matrix cell adhesion protein involved in tumorigenesis and matrix remodeling, has been associated with many cardiovascular diseases [34]; its detrimental role in abdominal [35] and ascending [36] aortic aneurysm has also been suggested.…”

Section: Discussionmentioning

confidence: 99%

Smooth muscle cells of human intracranial aneurysms assume phenotypic features similar to those of the atherosclerotic plaque

Coen

Burkhardt

Bijlenga

et al. 2013

Cardiovascular Pathology

View full text Add to dashboard Cite

“…17 Conversely, activated PBMCs grown on AAA-MSCs exhibited a higher BrdU incorporation rate (P<0.05, one-way ANOVA test) compared with cMSCs, whereas there were not significant differences with activated PBMCs cultured alone ( Figure 4D).…”

Section: Aaa-mscs Exert a Weak Immunosuppressive Effect On Pbmc Prolimentioning

confidence: 90%

Human Vascular Wall Mesenchymal Stromal Cells Contribute to Abdominal Aortic Aneurysm Pathogenesis Through an Impaired Immunomodulatory Activity and Increased Levels of Matrix Metalloproteinase-9

Ciavarella

Alviano

Gallitto

et al. 2015

Circ J

View full text Add to dashboard Cite

Elevated messenger RNA expression and plasma protein levels of osteopontin and matrix metalloproteinase types 2 and 9 in patients with ascending aortic aneurysms

Abstract: This study reveals an important role of osteopontin, MMP-2 and MMP-9 in the development of ascending and abdominal aortic aneurysm.

Cited by 33 publications

References 26 publications

Angiotensin II induces an increase in MMP-2 expression in idiopathic ascending aortic aneurysm via AT1 receptor and JNK pathway

Angiotensin II induces an increase in MMP-2 expression in idiopathic ascending aortic aneurysm via AT1 receptor and JNK pathway

Smooth muscle cells of human intracranial aneurysms assume phenotypic features similar to those of the atherosclerotic plaque

Human Vascular Wall Mesenchymal Stromal Cells Contribute to Abdominal Aortic Aneurysm Pathogenesis Through an Impaired Immunomodulatory Activity and Increased Levels of Matrix Metalloproteinase-9

Contact Info

Product

Resources

About