Elevated levels of kappa free light chains in CSF support the diagnosis of multiple sclerosis

Abstract

In this study, an elevated KFLC-index represented the most sensitive and specific quantitative diagnostic parameter for MS. As it is measured by automated, routinely available laboratory methods, KFLC quantitation can provide a rapid and reproduceable indication of intrathecal immunological processes supporting current MS diagnostic criteria.

Oligoclonal free light chains in cerebrospinal fluid as markers of intrathecal inflammation. Comparison with oligoclonal IgG

“…45 ). Up to now, there is no commercially available method explicitly designated for fLC measurement in the CSF, and absolute values obtained by various ELISAs or nephelometric assays modified for fLC measurement in the CSF are quite discrepant 5,33,[46][47][48][49][50] . Also, greater dilution of samples would require more sensitive, e.g.…”

Quantitative Proteomics and Metabolomics Analysis of Normal Human Cerebrospinal Fluid Samples*

“…Hence, the variation in protein abundance, e.g. immunoglobulin levels, which are known to be elevated in neuro-inflammatory diseases such as multiple sclerosis (23)(24)(25), is potentially far more extensive than in the nine well-defined individuals measured previously.…”

“…In the sample sets, there is a clear division that can be observed between proteins whose abundances vary highly among individuals and proteins that show a much more limited variation between individuals. Among the proteins that showed limited variation between individual CSF samples were serotransferrin (25% RSD in the original sample set, 18% RSD in the validation sample set and 50% RSD in the experimental sample set), tetranectin (21,18, and 52%, respectively), and gelsolin (24,19, and 58%, respectively). Proteins with high variation between individuals in all three sample sets included cadherin-13 (82% RSD in the original sample set, 60% RSD in the validation sample set and 143% RSD in the experimental sample set), contactin-2 (124%, 80 and 156%, respectively), and haptoglobin (135%, 84 and 182%, respectively).…”

Quantitative Proteomics and Metabolomics Analysis of Normal Human Cerebrospinal Fluid Samples

“…Hence, the variation in protein abundance, e.g. immunoglobulin levels, which are known to be elevated in neuro-inflammatory diseases such as multiple sclerosis (23)(24)(25), is potentially far more extensive than in the nine well-defined individuals measured previously.…”

“…In the sample sets, there is a clear division that can be observed between proteins whose abundances vary highly among individuals and proteins that show a much more limited variation between individuals. Among the proteins that showed limited variation between individual CSF samples were serotransferrin (25% RSD in the original sample set, 18% RSD in the validation sample set and 50% RSD in the experimental sample set), tetranectin (21,18, and 52%, respectively), and gelsolin (24,19, and 58%, respectively). Proteins with high variation between individuals in all three sample sets included cadherin-13 (82% RSD in the original sample set, 60% RSD in the validation sample set and 143% RSD in the experimental sample set), contactin-2 (124%, 80 and 156%, respectively), and haptoglobin (135%, 84 and 182%, respectively).…”