1988
DOI: 10.1172/jci113835
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Elevated glucose alters eicosanoid release from porcine aortic endothelial cells.

Abstract: Cultured porcine aortic endothelial cells were conditioned through two passages to mimic euglycemic and hyperglycemic conditions (5.2 mM, normal glucose; 15.6 mM, elevated glucose). After incubation with 1 ,uM I'4Clarachidonic acid for 24 h, the cells were stimulated with 1 ,uM A23187 for times up to 30 min. Uptake of ['4Clarachidonic acid and its distribution among cell lipids were unaffected by the increased glucose concentration. The release of eicosanoids from labeled cells and unlabeled cells was measured… Show more

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Cited by 48 publications
(35 citation statements)
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“…Free radicals inactivate endothelium-derived nitric oxide 27,28 , interfere with endothelial-dependent vasodilatation 27 and are produced during prostaglandin formation 29,30 . Increased prostaglandin synthesis has been demonstrated in models of both acute and chronic hyperglycemia 31,32 . Alternatively, hyperglycemia may lead directly to the formation of free radicals by glucose auto-oxidation 33 .…”
Section: Discussionmentioning
confidence: 99%
“…Free radicals inactivate endothelium-derived nitric oxide 27,28 , interfere with endothelial-dependent vasodilatation 27 and are produced during prostaglandin formation 29,30 . Increased prostaglandin synthesis has been demonstrated in models of both acute and chronic hyperglycemia 31,32 . Alternatively, hyperglycemia may lead directly to the formation of free radicals by glucose auto-oxidation 33 .…”
Section: Discussionmentioning
confidence: 99%
“…Autoxidation of glucose catalyzed by transition metals and glycosylation of proteins can also generate excess oxygen-derived free radicals (55)(56)(57). Lastly, experimental hyperglycemia in animals increases AA metabolism and eicosanoid synthesis (33,37,58,59). Enhanced eicosanoid synthesis results in increased production of oxygen-derived free radicals (60)(61)(62).…”
Section: Discussionmentioning
confidence: 99%
“…The haemodynamic effects of osmolality are largely unknown but, if anything, are rather characterized by vasodilation [34,35]. Second, a direct constrictive effect of glucose could also result from enhanced production of some prostanoïds [36,37], endothelin [38] or a stimulation of protein kinase C [39--41]. Third, since vessel caliber is also largely influenced by metabolites in the surrounding tissue, products of local glucose utilization in the muscle may have induced local vasoconstriction.…”
Section: Discussionmentioning
confidence: 99%