Elemental speciation by anion exchange and size exclusion chromatography with detection by inductively coupled plasma mass spectrometry with direct injection nebulization

Abstract

A direct injection nebulizer (DIN) is used with packed microcolumns for anion chromatography (AC) and size exclusion chromatography (SEC). Two Se species (SeOs2-and Se0d2-) are separated by AC with detection limits of 15 pg of Se. The isotope ratio 74Se/78Se can be measured with a relative standard deviation of 0.5% on 25 ngof each Se species during the actual chromatographic separation. Proteins in human serum are separated by SEC without sample pretreatment. The metals present in each molecular weight fraction are determined by ICP-MS with detection limits of 0.5-3 pgof metal. Theseabsolute detection limits are 1-2 orders of magnitude better than those obtained with conventional nebulizers.

Speciation analysis by small-bore HPLC coupled to ICP-MS

“…[21] for the separation of selenocompounds. Then, it has been used for biological applications such as metalloproteins separation [30] and copper speciation [42].…”

Determination of plutonium isotopes in waters and environmental solids: A review

“…However, in order to reduce the sample consumption and consequently minimize instrumental radioactivity contamination and reduce waste generation, high efficiency micronebulization might be hyphenated with ICP-MS for precise and accurate determination of ultra trace radionuclides as well as their isotopic ratios. Various low-consumption nebulisers, such as the microconcentric nebulizer (MCN) [102,103], the high efficiency nebulizer (HEN) [104][105][106][107][108][109], the direct injection nebulizer (DIN) [110][111][112], oscillating capillary nebulizer (OCN) [113,114] by using similar operation conditions for SF-ICP-MS but without application of Ar-N 2 mixed gas plasma [71]. However, the 241 Pu results had relatively large uncertainty if the intensity of signals is rather low.…”

High throughput sample introduction system for the analysis of drinking waters and wastewaters by ICP-MS

“…Direct injection nebulizers (DIN) 28,29 and direct injection high efficiency nebulizers (DIHEN) 30,31 improve the sample transport efficiency to 100%, even at relatively low flow rates, and do not require a spray chamber, thereby decreasing the dead volume, increasing the response time, and reducing memory effects.…”