Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage

Abstract

As a powerful gene editing tool, the kinetic mechanism of CRISPR/Cas9 has been the focus to its further application. Initial cleavage events as the first domino followed by nuclease end...

Isotope-encoded tetrahedral DNA for multiple SARS-CoV-2 variant diagnosis

“…Intriguingly, molecular interpretations encoded by multiple isotopes exhibit high sensitivity, wide dynamic range, 18 and, most importantly, no signal overlap compared to most used fluorophore tags. 19,20 Efforts have been made in multiplexed assays with respect to nucleic acid 21,22 and protein 23,24 markers for molecular diagnostics, and mass cytometry (CyTOF), 25 spatial single nuclear metabolomics (SEAM) 26 have showcasted superior high-dimensional barcoding ability. Overall, isotope-encoding mass spectrometry is ideally suited for multicomponent assays.…”

Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation

“…Endowed with high throughput and resolution, single particle ICP-MS (sp-ICP-MS) has gained great attention in multiplex biological practice and single-cell analysis. [18][19][20][21][22][23][24] Based on the theory in a typical sp-ICP-MS analysis, nanoparticles are injected one by one into the detector where only one nanoparticle is measured during each reading period. Beneting from ample metal content in one nanoparticle, sensitivity is largely improved in heterogeneous bioassays where absolute quantications can be easily achieved by measuring the total numbers of target-specic nanoparticles.…”

Single-Nanoparticle Differential Immunoassay for Multiplexed Gastric Cancer Biomarker Monitoring

“…41 To improve the diagnostic performance, the biomarker combination strategy, which can simultaneously provide comprehensive information on multiple cancer biomarkers, has the potential to solve the above-mentioned concerns. 42,43 As a fast-developing state-ofthe-art technology, sp-ICP-MS-based multiplexed proposal with the biomarker combination strategy is thus promising to help assist GC diagnosis.…”

“…Unfortunately, when several biomarkers are evaluated separately as usual, there is an occurrence of obtaining inconsistent results, which easily bring misinterpretations of the gastric conditions . To improve the diagnostic performance, the biomarker combination strategy, which can simultaneously provide comprehensive information on multiple cancer biomarkers, has the potential to solve the above-mentioned concerns. , As a fast-developing state-of-the-art technology, sp-ICP-MS-based multiplexed proposal with the biomarker combination strategy is thus promising to help assist GC diagnosis.…”