Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation

Abstract

Explicit interpretation of heterogeneity between prostate-specific antigen (PSA) subtypes is essential for prostate cancer differentiation during different disease courses, whereas the universal protocol with uniform criteria is still lacking across...

Single Nanoparticle Counting-Based Liquid Biopsy for Cancer Diagnosis

“…It can distinguish the signal differences of individual nanoparticles in terms of size and particle concentration, which in turn prevents these differences from being masked in the average signal in solution. , Meanwhile, due to a large amount of detectable atoms in one nanoparticle, SP-ICPMS can further improve the sensitivity and achieve amplification-free analysis with high sensitivity . A series of explorations of bioanalytical strategies based on SP-ICPMS have been successfully carried out, confirming the unique advantages of SP-ICPMS. − A single magnetic bead nanoplatform was applied to the detection of two prostate cancer-associated biomarkers and the differentiation of prostate disease . Considering its great application potential as a multiplexed detection tool, it can provide a reliable and solid foundation for the establishment of the multiplexed miRNA analysis platform with high sensitivity.…”

Single-Nanoparticle Differential Immunoassay for Multiplexed Gastric Cancer Biomarker Monitoring

“…37 However, the reports on screening nanoparticles with the suitable diameter for highly efficient immunoassay are rare. 18 A washfree strategy with high outputs meeting the above-mentioned requirements is never out of date to further promote the development of heterogeneous immunoassay.…”

“…Synthesis and Labeling of Noble Metal Nanoparticles. PtNPs with four diameters (18,34,51, and 62 nm) were synthesized according to the previous experience. 44 First, 5 nm Pt seeds were synthesized by successively adding a mixture of trisodium citrate and citrate acid, 1% trisodium citrate, and KBH 4 to 50 mL of boiling solution containing chloroplatinic acid.…”

“…In common heterogeneous immunoassay design, the washing step is always an inevitable separation strategy for the removal of unbound probes from the bound ones with the demand of extra labor, lab skills, and time costs. − More importantly, the washing step is like a hidden double-edged sword. On the one hand, the increase of washing rounds makes the signals more stable with less interference, but on the other hand, the effective outputs are ineluctably reduced due to the loss of probes during the washing steps, challenging the development of lower limit-of-detection analysis. − The deficiency of multiplexed assays is also a heated topic where further optimization and improvement are desired for simultaneously providing multicomponent evaluations of malignant diseases in a facile way …”

“…Moreover, since nanoparticles are applied for sensitive analyte counting, the wise selection of a proper elemental content (i.e., suitable size) nanoparticle probes on the solid support is a key prerequisite . However, the reports on screening nanoparticles with the suitable diameter for highly efficient immunoassay are rare . A wash-free strategy with high outputs meeting the above-mentioned requirements is never out of date to further promote the development of heterogeneous immunoassay.…”