Elektronik für Ingenieure und Naturwissenschaftler

Hering, Ekbert; Bressler, Klaus; Gutekunst, Jürgen

doi:10.1007/978-3-642-05499-0

Cited by 16 publications

(13 citation statements)

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“…The specific electrical conductivity of platinum is quite low (κ = 10.2×10 6 S/m) compared to that of gold (κ = 49×10 6 S/m) [9]. Since the platinum electrodes showed a high resistivity (data not shown) due to their lower conductance, a sandwich structure with a gold and a covering platinum layer was used for the experiments regarding orientation of the cell cleavage planes.…”

Section: Methodsmentioning

confidence: 99%

Electrical Stimulation of NIH-3T3 Cells with Platinum-PEDOT-Electrodes Integrated in a Bioreactor

Blume¹,

Müller-Wichards²,

Goepfert³

et al. 2013

TOBEJ

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The objective of this work involves the development and integration of electrodes for the electrical stimulation of cells within a bioreactor. Electrodes need to fit properties such as biocompatibility, large reversible charge transfer and high flexibility in view of their future application as implants on the tympanic membrane. Flexible thin-film platinum-poly(3,4-ethylene-dioxythiophene)-electrodes on a poly(ethylene terephthalate)-foil manufactured using microsystems technology were integrated into a bioreactor based on the design of a 24 well plate. The murine fibroblast cell line NIH-3T3 was cultured on the foil electrodes and the cells were stimulated with direct voltage and unipolar pulsed voltage. The amplitude, the pulse length and the ratio of pulse to pause were varied. The stimulated cells were stained in order to determine the angle between the cell cleavage plane of the dividing cells and the vector of the electric field. These angles were subsequently used to calculate the polarization index, which is a measure of the orientation of the metaphase plane of dividing cells that occurs for example during wound healing or embryonic morphogenesis.

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Section: Methodsmentioning

confidence: 99%

Electrical Stimulation of NIH-3T3 Cells with Platinum-PEDOT-Electrodes Integrated in a Bioreactor

Blume¹,

Müller-Wichards²,

Goepfert³

et al. 2013

TOBEJ

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“…The probe head consists of a name-giving needle and seven bare glass fibers for illumination and detection [13]. Glass fibers can be used to transport light from the probe head to the spectrometer or detection unit [29,30]. The real time detection of malignant tissue in in vivo measurements is possible.…”

Section: Combination Techniques: Uv/vis/nir/fluorescencementioning

confidence: 99%

Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

2020

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This paper provides an overview of how molecule-sensitive, spatially-resolved technologies can be applied for monitoring and measuring in microchannels. The principles of elastic light scattering, fluorescence, near-infrared, mid-infrared, and Raman imaging, as well as combination techniques, are briefly presented, and their advantages and disadvantages are explained. With optical methods, images can be acquired both scanning and simultaneously as a complete image. Scanning technologies require more acquisition time, and fast moving processes are not easily observable. On the other hand, molecular selectivity is very high, especially in Raman and mid-infrared (MIR) scanning. For near-infrared (NIR) images, the entire measuring range can be simultaneously recorded with indium gallium arsenide (InGaAs) cameras. However, in this wavelength range, water is the dominant molecule, so it is sometimes necessary to use complex learning algorithms that increase the preparation effort before the actual measurement. These technologies excite molecular vibrations in a variety of ways, making these methods suitable for specific products. Besides measurements of the fluid composition, technologies for particle detection are of additional importance. With scattered light techniques and evaluation according to the Mie theory, particles in the range of 0.2–1 µm can be detected, and fast growth processes can be observed. Local multispectral measurements can also be carried out with fiber optic-coupled systems through small probe heads of approximately 1 mm diameter.

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“…With an estimated width of = 20 ns of the electronic response of a fast silicon APD, this leads to an electronic current of I p = 2800 e À /20 ns ' 22 nA. When operated in the linear regime, the avalanche effect of an APD provides a current amplification of 10-1000Â (Hering et al, 2005); a single-photon signal of I p = 250 Â 22 nA = 5.5 mA is therefore assumed as a starting point for the design of the subsequent pulse amplification and processing electronics.…”

Section: Technical Realisationmentioning

confidence: 99%

Pulse-resolved multi-photon X-ray detection at 31 MHz based on a quadrant avalanche photodiode

et al. 2014

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The technical realisation and the commissioning experiments of a high-speed X-ray detector based on a quadrant avalanche silicon photodiode and highspeed digitizers are described. The development is driven by the need for X-ray detectors dedicated to time-resolved diffraction and imaging experiments, ideally requiring pulse-resolved data processing at the synchrotron bunch repetition rate. By a novel multi-photon detection scheme, the exact number of X-ray photons within each X-ray pulse can be recorded. Commissioning experiments at beamlines P08 and P10 of the storage ring PETRA III, at DESY, Hamburg, Germany, have been used to validate the pulse-wise multi-photon counting scheme at bunch frequencies !31 MHz, enabling pulse-by-pulse readout during the PETRA III 240-bunch mode with single-photon detection capability. An X-ray flux of ! 3.7 Â 10 9 photons s À1 can be detected while still resolving individual photons at low count rates.

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Elektronik für Ingenieure und Naturwissenschaftler

Cited by 16 publications

References 0 publications

Electrical Stimulation of NIH-3T3 Cells with Platinum-PEDOT-Electrodes Integrated in a Bioreactor

Electrical Stimulation of NIH-3T3 Cells with Platinum-PEDOT-Electrodes Integrated in a Bioreactor

Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

Pulse-resolved multi-photon X-ray detection at 31 MHz based on a quadrant avalanche photodiode

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