Elektronenmikroskopische Untersuchungen �ber die Entwicklung des Herpesvirus hominis in Kulturzellen

Electron microscopic studies of herpes simplex virus type 1 infection of macrophages, T- and B-lymphocytes of mice

“…This is in agreement with SIEG~T and FALKE (27). The experiments showed that murine maerophages (phagocytic monoeytes) ingest the virus particles.…”

“…In B-lymphoeytes maturation occurs by budding of NC through the altered inner nuelear membrane as first described by SI~G~I~T and FaLKE (27). In maerophages and T-lymphocytes such occurrences could not be observed.…”

Lipids in Viruses

“…Several important findings were noted. First, the level of radioactivity of the inner nuclear membrane and that of purified virus closely resembled each other, confirming the morphological studies of Siegert and Falke (1966), which indicated that the virus acquired its envelope from this membrane. Second, the specific activities of the nuclear membrane and virion were quite different, suggesting to Ben-Porat and Kaplan (1972) that the viral envelope was derived from more metabolically active sites of the inner nuclear membrane than the remainder of the nuclear envelope.…”

“…Herpesviruses. In general envelopment proceeds by budding through a membrane; here the inner nuclear membrane is preferred (Siegert and Falke, 1966;Darlington and Moss, 1968). However, any membrane may be used, including the Golgi apparatus, endoplasmic reticulum, and occasionally the plasma membrane; in some cases double membranes are formed.…”

“…Both naked and enveloped herpesvirus particles are found in infected cells, but only enveloped particles are found in extracellular fluids (Roiznlan et al, 1968). It is not altogether clear whether only enveloped particles are infectious (Siegert and Falke, 1966;Watson et al, 1964). The question may therefore be raised of why herpesvirus needs an envelope.…”