Elektronenmikroskopische Untersuchung des Vitamin-E-Mangel-Pigmentes im Myometrium der Ratte

Gedigk, P.; Wessel, W.

doi:10.1007/bf00957668

Cited by 25 publications

(3 citation statements)

References 21 publications

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“…Occasionally, large (5-8 As) inclusions showing reaction product on an area of what appears to be cell debris are seen in sections of interstitial cells (Figs. [18][19][20]. Interlocking cell processes are seen around each inclusion.…”

Section: Observationsmentioning

confidence: 99%

“…The large inclusions seen occasionally (Figs. [18][19][20] are difficult to interpret. They could be intrusions of macrophage cytoplasm indenting the interstitial cells.…”

Section: Figure 16mentioning

confidence: 99%

“…Acid phosphatase has also been demonstrated at the electron microscope level in the lipofuscin of mouse mammary gland (29). The morphology of the pigment has been shown in electron micrographs of many cell types (2,4,18,22,23,36,41,45), including human testicular interstitial cells (16). The cytochemical localiza-tion of acid phosphatase suggests that lipofuscin is a residue of lysosomal digestion.…”

Section: Introductionmentioning

confidence: 99%

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Localization of Acid Phosphatase in Lipofuscin Granules and Possible Autophagic Vacuoles in Interstitial Cells of the Guinea Pig Testis

Frank

Christensen

1968

The Journal of Cell Biology

159

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The intracellular localization of acid phosphatase in guinea pig testicular interstitial cells was investigated by incubating nonfrozen thick sections of glutaraldehyde-perfused testis in a modified Comori medium and preparing the tissue for electron microscopy. Lipofuscin pigment granules in these cells contain dense pigment, granular matrix, and often a lipid droplet. Reaction product is seen in the matrix of the pigment granules, and they may therefore be called residual bodies. At least some of the dense pigment appears to be derived from myelin figures and membrane whorls, since suitable intermediates can be seen. Lipid droplets found free in the cytoplasm are another possible source of pigment. In both cases the chemical mechanism is presumed to be autoxidation of unsaturated lipid. Acid phosphatase is present in the inner cisterna of Golgi elements. Enzyme activity also appears in possible autophagic vacuoles bounded by double membranes; the reaction product lies between the membranes. Consideration of the enzyme as a tracer suggests that the autophagic vacuoles are derived from the Golgi complex. Possible stages in the formation of these vacuoles by the inner Golgi cisternae are observed.

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Section: Observationsmentioning

confidence: 99%

“…The large inclusions seen occasionally (Figs. [18][19][20] are difficult to interpret. They could be intrusions of macrophage cytoplasm indenting the interstitial cells.…”

Section: Figure 16mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localization of Acid Phosphatase in Lipofuscin Granules and Possible Autophagic Vacuoles in Interstitial Cells of the Guinea Pig Testis

Frank

Christensen

1968

The Journal of Cell Biology

159

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Histochemical and ultrastructural observations on brown degeneration of human intervertebral disc

Ishi‐i

Tsuji

Sano

et al. 1991

Journal Orthopaedic Research

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Thirty-eight fresh human intervertebral discs collected during anterior interbody fusion surgery were histochemically and ultrastructurally analyzed for pigments. Macroscopically, five stages of degeneration were classified according to the color, fibrosis, and fragility of the nucleus pulposus of the discs. In order to demonstrate lipofuscin granules, specimens were subjected to special staining procedures, including carbol fuchsin lipofuscin stain, the Schmorl's reaction, and autofluorescence. Lipofuscin granules were distributed from the inner layer of the annulus fibrosus to the nucleus pulposus. Such granules were numerous in cases of slight or severe degeneration, whereas fewer granules were found in cases of moderate degeneration. However, the stage of macroscopic degeneration of the intervertebral disc did not necessarily correlate with the incidence of lipofuscin granules. By ultrastructural observation, the morphological features of the components of the intervertebral disc and the ultrastructure of the lipofuscin granule were clarified. The ultrastructure of the "brown degeneration" disc exhibited markedly increased amorphous electron-dense bodies located among collagen fibrils in the matrix.

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Brown pigmentation (lipofuscinosis) of canine intestinal muscularis

Cordes

Mosher

1966

J. Pathol.

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Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component of the Escherichia coli cell-free system, i.e., ribosomes, soluble ribonucleic acid (sRNA), polyuridylic acid (poly U), and S-100 (supernatant enzymes), only the ribosomal-bound antibiotic was inhibitory to the cell-free assay. Experiments designed to further localize the site of inhibition to either the 50S (Svedberg) or the 30S ribosomal subunit were not conclusive. Tritiated tetracycline (7-H(3)-tetracycline) was bound to isolated 50S ribosomes, and these were recombined with 30S subunits to form 70S ribosomes. When these ribosomes were dissociated and the subunits reisolated, the antibiotic was found with both the 50S and the 30S particles. The same results were observed when the tetracycline was initially bound to the 30S subunit.

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Elektronenmikroskopische Untersuchung des Vitamin-E-Mangel-Pigmentes im Myometrium der Ratte

Cited by 25 publications

References 21 publications

Localization of Acid Phosphatase in Lipofuscin Granules and Possible Autophagic Vacuoles in Interstitial Cells of the Guinea Pig Testis

Localization of Acid Phosphatase in Lipofuscin Granules and Possible Autophagic Vacuoles in Interstitial Cells of the Guinea Pig Testis

Histochemical and ultrastructural observations on brown degeneration of human intervertebral disc

Brown pigmentation (lipofuscinosis) of canine intestinal muscularis

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