Electrostatic manipulation of DNA in microfabricated structures

Washizu, Masao; Kurosawa, Osamu

doi:10.1109/28.62403

Cited by 319 publications

(213 citation statements)

References 19 publications

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“…Many of these techniques are relatively time consuming, costly and often lead to significant loss of the analyte. AC electrokinetic techniques, like dielectrophoresis (DEP) have been attractive because they allow cells [9][10][11], hmw-DNA biomarkers [12][13][14][15] and proteins [16] to be rapidly isolated and concentrated into specific microscopic locations. Dielectrophoresis (DEP) is an induced motion of particles produced by the dielectric differences between the particles and media in an AC electric field [17,18].…”

Section: Introductionmentioning

confidence: 99%

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Krishnan

Heller

2009

Journal of Biophotonics

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Journal of BIOPHOTONICSIn biomedical research and diagnostics it is a challenge to isolate and detect low levels of nanoparticles and nanoscale biomarkers in blood and other biological samples. While highly sensitive epifluorescent microscope systems are available for ultra low level detection, the isolation of the specific entities from large sample volumes is often the bigger limitation. AC electrokinetic techniques like dielectrophoresis (DEP) offer an attractive mechanism for specifically concentrating nanoparticles into microscopic locations. Unfortunately, DEP requires significant sample dilution thus making the technology unsuitable for biological applications. Using a microelectrode array device, special conditions have been found for the separation of hmw-DNA and nanoparticles under high conductance (ionic strength) conditions. At AC frequencies in the 3000-10 000 Hz range, 10 mm microspheres and human T lymphocytes can be isolated into the DEP low field regions, while hmw-DNA and nanoparticles can be concentrated into microscopic high field regions for subsequent detection using an epifluorescent system.Final separation and detection of 150 ng/mL OliGreen fluorescent stained high molecular weight (hmw) DNA in 1 Â TBE buffer (109 mS/m). The DEP field was applied at 10 000 Hz and 10 volts pk-pk for 5 minutes to group of nine microelectrodes. The green fluorescent image (Ex 505 nm, Em 520 nm) shows fluorescence from the OliGreen stained hmw DNA concentrated on the 80-micron diameter microelectrodes with the far right column of unactivated microelectrodes showing no concentration of hmw-DNA.

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Section: Introductionmentioning

confidence: 99%

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Krishnan

Heller

2009

Journal of Biophotonics

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“…For any given molecule, a relaxation time () can usually be calculated by the frequency response of the induced dipole which depends on the speed at which charge carriers can rearrange themselves in response to an electric field. The first studies of DNA dielectrophoresis were carried out by Washizu et al who found that DNA could be manipulated and stretched at high electric field intensities between frequencies of 40kHz and 2MHz [2]. Despite some studies into the underlying mechanism on a molecular level for DNA dielectrophoresis, a fully comprehensive understanding has not yet been provided.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution

Mohamad

Jeynes

Hughes

2014

IEEE Trans.on Nanobioscience

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In this paper we use dielectrophoresis to determine the electrical properties of poly(dG)-poly(dC) (GC) and poly(dA)-poly(dT) (AT) DNA in solution. The molecules show different conduction mechanisms, with GC DNA predominately through the molecule, but that conduction through the counterion cloud surrounding the molecule in solution is the more significant conductor in AT DNA. Moreover, the effect of frequency on DNA lengths differing by four orders of magnitude show no difference in the dielectrophoretic response.

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“…Similar bacterial species and alive cells can be separated by using dielectrophoretic effects [1][2][3]. Combination of electrophoretic and dielectrophoretic forces allows trapping and delicate manipulating DNA and polymer particles on planar microelectrodes [4,5]. Effects of dielectric particles manipulation by an electric field lie in a base of operation of e-papers and electrophoretic displays [6,7].…”

Section: Introductionmentioning

confidence: 99%

Drag of Micro-Particles by an Extended Nematic-Isotropic Interface

West¹,

Zhang²,

Glushchenko³

et al. 2004

Molecular Crystals and Liquid Crystals

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We studied the behaviour of polymer particles in a moving interface between the nematic (N) and isotropic (I) phases of a nematic liquid crystal (LC). We showed that the NI-interface is extended (E) and has a layered N-I-N structure in the vertical cross-section of the sample; the wedge of the isotropic phase is bounded by the nematic phase, which is limited by the cell substrates. The minimum of the cell free energy defines the position of particles in the interface region. We find that the preferable position of the particle is at the vertex of the wedge formed by the isotropic phase. The particles are captured by the vertex line and follow the interface when it moves.

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Electrostatic manipulation of DNA in microfabricated structures

Cited by 319 publications

References 19 publications

An AC electrokinetic method for enhanced detection of DNA nanoparticles

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution

Drag of Micro-Particles by an Extended Nematic-Isotropic Interface

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