Electrostatic Interactions Affect Nanoparticle-Mediated Toxicity to Gram-Negative Bacterium <i>Pseudomonas aeruginosa</i> PAO1

Feris, Kevin P.; Otto, Caitlin C.; Tinker, Juliette; Wingett, Denise; Punnoose, Alex; Thurber, Aaron; Kongara, Madhu; Sabetian, Maryam; Quinn, Bonnie; Hanna, Charles B.; Pink, David A.

doi:10.1021/la903491z

Cited by 148 publications

(83 citation statements)

References 54 publications

(98 reference statements)

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“…It is in this context that the recent reports of the high cytotoxic effects of ZnO nanoparticles (NP) to both prokaryotic and eukaryotic systems pose a grave concern. 4,8−19 ZnO NP are among the most toxic engineered nanomaterials, especially among oxide NP. 14,20−22 …”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of ZnO Nanoparticles Can Be Tailored by Modifying Their Surface Structure: A Green Chemistry Approach for Safer Nanomaterials

Punnoose

Dodge

Rasmussen

et al. 2014

ACS Sustainable Chem. Eng.

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133

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ZnO nanoparticles (NP) are extensively used in numerous nanotechnology applications; however, they also happen to be one of the most toxic nanomaterials. This raises significant environmental and health concerns and calls for the need to develop new synthetic approaches to produce safer ZnO NP, while preserving their attractive optical, electronic, and structural properties. In this work, we demonstrate that the cytotoxicity of ZnO NP can be tailored by modifying their surface-bound chemical groups, while maintaining the core ZnO structure and related properties. Two equally sized (9.26 ± 0.11 nm) ZnO NP samples were synthesized from the same zinc acetate precursor using a forced hydrolysis process, and their surface chemical structures were modified by using different reaction solvents. X-ray diffraction and optical studies showed that the lattice parameters, optical properties, and band gap (3.44 eV) of the two ZnO NP samples were similar. However, FTIR spectroscopy showed significant differences in the surface structures and surface-bound chemical groups. This led to major differences in the zeta potential, hydrodynamic size, photocatalytic rate constant, and more importantly, their cytotoxic effects on Hut-78 cancer cells. The ZnO NP sample with the higher zeta potential and catalytic activity displayed a 1.5-fold stronger cytotoxic effect on cancer cells. These results suggest that by modifying the synthesis parameters/conditions and the surface chemical structures of the nanocrystals, their surface charge density, catalytic activity, and cytotoxicity can be tailored. This provides a green chemistry approach to produce safer ZnO NP.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity of ZnO Nanoparticles Can Be Tailored by Modifying Their Surface Structure: A Green Chemistry Approach for Safer Nanomaterials

Punnoose

Dodge

Rasmussen

et al. 2014

ACS Sustainable Chem. Eng.

Self Cite

133

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“…3 The toxicity and antimicrobial effects of ZnO-NPs on microorganisms in activated sludge and on algae and various aquatic species have previously been reported. [8][9][10][11] Industrially released ZnO-NPs could adversely impact the removal of biological pollutants in WWTPs because the microorganisms * Author to whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Effects of Sulfidation on ZnO Nanoparticle Dissolution and Aggregation in Sulfate-Containing Suspensions

Rasool¹,

Lee²

2015

j nanosci nanotechnol

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Industrial metal oxide nanoparticles (NPs) have recently attracted considerable attention because of their potentially hazardous impacts on ecosystems and microbial colonies in biological wastewater treatment plants. NPs dissolution and aggregation greatly determine the fate of such NPs in the environment and are relevant to their potential toxicities. Hence, we investigated the effects of sulfate on the dissolution and aggregation of ZnO nanoparticles (ZnO-NPs). In addition, ZnO-NPs were sulfidized at different sulfide concentrations in an anaerobic abiotic environment to investigate the effects of sulfidation on ZnO-NPs aggregation and solubility. Increasing the sulfate concentration from 0 to 200 mg/L significantly increased ZnO-NPs dissolution from 3.99 to 6.18 mg Zn(2+)/L, whereas ZnO-NPs sulfidation reduced the Zn(2+) dissolution rate from 1.82 mg Zn(2+)/L for pristine ZnO-NPs to 0.59 mg Zn2+/L for sulfidized ones. Increasing the sulfate concentration and the sulfidation of the ZnO-NPs induced aggregation by suppressing electrostatic repulsion. The results indicate that the sulfidation of ZnO-NPs prevents the particle dissolution and is an attractive method of reducing their antimicrobial activity.

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“…13 Many studies reported that direct spatial contact between NPs and cell surface is necessary for manifestation of the cytotoxicity, 13,14,16,19 and their interaction is central to the cytotoxicity of NPs. 13,20,21 An apparent mechanism relies on direct damages, either physical (e.g., pitting 22 ) or chemical (e.g., oxidative stress 13 ), of NPs to cell surface (cell wall or cell membrane), which can result in death of the cell. 22 Prolonged contact between the cell and the NPs likely alters the cellular surface properties or integrity 23,24 and triggers the internalization of NPs through endocytosis 25 or direct penetration.…”

Section: ■ Introductionmentioning

confidence: 99%

Surface Interactions Affect the Toxicity of Engineered Metal Oxide Nanoparticles toward Paramecium

Chen

Zhang

et al. 2012

Chem. Res. Toxicol.

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To better understand the potential impacts of engineered metal oxide nanoparticles (NPs) in the ecosystem, we investigated the acute toxicity of seven different types of engineered metal oxide NPs against Paramecium multimicronucleatum, a ciliated protozoan, using the 48 h LC(50) (lethal concentration, 50%) test. Our results showed that the 48 h LC(50) values of these NPs to Paramecium ranged from 0.81 (Fe(2)O(3) NPs) to 9269 mg/L (Al(2)O(3) NPs); their toxicity to Paramecium increased as follows: Al(2)O(3) < TiO(2) < CeO(2) < ZnO < SiO(2) < CuO < Fe(2)O(3) NPs. On the basis of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, interfacial interactions between NPs and cell membrane were evaluated, and the magnitude of interaction energy barrier correlated well with the 48 h LC(50) data of NPs to Paramecium; this implies that metal oxide NPs with strong association with the cell surface might induce more severe cytotoxicity in unicellular organisms.

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Electrostatic Interactions Affect Nanoparticle-Mediated Toxicity to Gram-Negative Bacterium Pseudomonas aeruginosa PAO1

Cited by 148 publications

References 54 publications

Cytotoxicity of ZnO Nanoparticles Can Be Tailored by Modifying Their Surface Structure: A Green Chemistry Approach for Safer Nanomaterials

Cytotoxicity of ZnO Nanoparticles Can Be Tailored by Modifying Their Surface Structure: A Green Chemistry Approach for Safer Nanomaterials

Effects of Sulfidation on ZnO Nanoparticle Dissolution and Aggregation in Sulfate-Containing Suspensions

Surface Interactions Affect the Toxicity of Engineered Metal Oxide Nanoparticles toward Paramecium

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