Electrostatic interaction effects on the size distribution of self-assembled giant unilamellar vesicles

Karal, Mohammad Abu Sayem; Ahmed, Marzuk; Levadny, Victor; Belaya, Marina; Ahamed, Md. Kabir; Rahman, M. Mostafizur; Shakil, Md. Mostofa

doi:10.1103/physreve.101.012404

Cited by 21 publications

(35 citation statements)

References 60 publications

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“…The size distribution of the unpuried and puried GUVs prepared using different conditions has been reported previously. 60,66 The size range for the unpuried GUVs was 3.0-60 mm, and the number of smaller vesicles with sizes of less than 10 mm was large. On the other hand, the size range for the puried GUVs was 7.0-60 mm, and the number of smaller vesicles with sizes of less than 10 mm was very small.…”

Section: Methodsmentioning

confidence: 99%

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

et al. 2021

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Section: Methodsmentioning

confidence: 99%

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

et al. 2021

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“…The analysis of the size distribution of vesicles in such population provides important information about the processes of GUVs formation. There were a number of experimental and theoretical studies dealing with this problem which elucidated the basic principles of the spontaneous lipid vesiculation [23][24][25]. Currently, it is commonly accepted that the equilibrium size distribution of a vesicle population as well as the stability of each vesicle in the population are determined by a competition between total curvature energy of all vesicles and various sources of entropy of the system, such as vesicle translation and bilayer undulation.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, it is commonly accepted that the equilibrium size distribution of a vesicle population as well as the stability of each vesicle in the population are determined by a competition between total curvature energy of all vesicles and various sources of entropy of the system, such as vesicle translation and bilayer undulation. Additionally, it was shown that the membrane bending modulus was a key factor that determined vesicle size distributions [25].…”

Section: Introductionmentioning

confidence: 99%

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

et al. 2022

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The influence of cholesterol fraction in the membranes of giant unilamellar vesicles (GUVs) on their size distributions and bending moduli has been investigated. The membranes of GUVs were synthesized by a mixture of two elements: electrically neutral lipid 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol and also a mixture of three elements: electrically charged lipid 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), DOPC and cholesterol. The size distributions of GUVs have been presented by a set of histograms. The classical lognormal distribution is well fitted to the histograms, from where the average size of vesicle is obtained. The increase of cholesterol content in the membranes of GUVs increases the average size of vesicles in the population. Using the framework of Helmholtz free energy of the system, the theory developed by us is extended to explain the experimental results. The theory determines the influence of cholesterol on the bending modulus of membranes from the fitting of the proper histograms. The increase of cholesterol in GUVs increases both the average size of vesicles in population and the bending modulus of membranes.

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“…To investigate the effects of dual filtration with a combination of polycarbonate membranes on the size and distribution of GUVs, 12 μm pores polycarbonate membrane was used in filter holder 1 and 10 μm pores polycarbonate membrane was used in filter holder 2 (case i). We analyzed the size distribution histograms with the well-known lognormal distribution [35], which was also previously used for fitting the size distribution of GUVs [24,36]. The lognormal distribution is described as follows:…”

Section: Dual Filtration With a Combination Of 12 And 10 μM Pores Polycarbonate Membranesmentioning

confidence: 99%

A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration

et al. 2021

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A new purification technique is developed for obtaining distribution of giant unilamellar vesicles (GUVs) within a specific range of sizes using dual filtration. The GUVs were prepared using well known natural swelling method. For filtration, different combinations of polycarbonate membranes were implemented in filter holders. In our experiment, the combinations of membranes were selected with corresponding pore sizes–(i) 12 and 10 μm, (ii) 12 and 8 μm, and (iii) 10 and 8 μm. By these filtration arrangements, obtained GUVs size distribution were in the ranges of 6−26 μm, 5–38 μm and 5–30 μm, respectively. In comparison, the size distribution range was much higher for single filtration technique, for example, 6−59 μm GUVs found for a membrane with 12 μm pores. Using this technique, the water-soluble fluorescent probe, calcein, can be removed from the suspension of GUVs successfully. The size distributions were analyzed with lognormal distribution. The skewness became smaller (narrow size distribution) when a dual filtration was used instead of single filtration. The mode of the size distribution obtained in dual filtration was also smaller to that of single filtration. By continuing this process of purification for a second time, the GUVs size distribution became even narrower. After using an extra filtration with dual filtration, two different size distributions of GUVs were obtained at a time. This experimental observation suggests that different size specific distributions of GUVs can be obtained easily, even if GUVs are prepared by different other methods.

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Electrostatic interaction effects on the size distribution of self-assembled giant unilamellar vesicles

Cited by 21 publications

References 60 publications

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles

A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration

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