Electrostatic effects on the partitioning of spherical colloids between dilute bulk solution and cylindrical pores

Smith, Frank; Deen, William M.

doi:10.1016/0021-9797(83)90371-5

Cited by 139 publications

(113 citation statements)

References 14 publications

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“…1c) 29,30 . The electric potential remains negative in the whole nanochannel, resulting in an electrostatic exclusion of negatively charged molecules.…”

Section: Design Of Anisotropic Nanofilter Arraymentioning

confidence: 99%

“…The designed structural anisotropy of the ANA is critical to the continuous-flow separation, which is not readily possible with a random isotropic sieving medium (e.g., gel, liquid gel, and ampholytes). Moreover, the ANA allows for various sieving mechanisms (e.g., Ogston sieving 22-24 , entropic trapping 25-28 , and electrostatic sieving 29,30 ) to take effect for separation of biomolecules of very broad biological size scales and based on different molecular properties, which is a clear advantage over the conventional and nonconventional techniques discussed above. The continuous-flow operation of the ANA further permits continuous-harvesting of purified biomolecules for downstream analysis, rendering the ANA a promising generic sieving structure for an integrated biomolecule sample preparation and analysis system.…”

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confidence: 99%

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A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

et al. 2007

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Microfabricated regular sieving structures hold great promise as an alternative to gels to improve biomolecule separation speed and resolution. In contrast to disordered gel porous networks, these regular structures also provide well-defined environments ideal for study of molecular dynamics in confining spaces. However, previous regular sieving structures have been limited for separation of long DNA molecules, and separation of smaller, physiologically-relevant macromolecules, such as proteins, still remains as a challenge. Here we report a microfabricated anisotropic sieving structure consisting of a two-dimensional periodic nanofluidic filter array (Anisotropic Nanofilter Array: ANA). The designed structural anisotropy in the ANA causes different-sized or -charged biomolecules to follow distinct trajectories, leading to efficient separation. Continuous-flow sizebased separation of DNA and proteins as well as electrostatic separation of proteins were achieved, thus demonstrating the potential of the ANA as a generic molecular sieving structure for an integrated biomolecule sample preparation and analysis system. Efficient methods of separating and purifying biomolecules from a complex mixture are of utmost importance in biology and biomedical engineering. Currently, nucleic acids and proteins are routinely separated based on size by gel filtration chromatography or by gel electrophoresis 1,2 . Both techniques use gelatinous materials that consist of a cross-linked, three-dimensional pore network, where the sieving interaction with the migrating macromolecules determines the separation efficiency 3,4 . Both gel-based techniques represent the current standard for size-based macromolecule separation. However poor separation resolution in gel filtration chromatography and difficult sample recovery with gel electrophoresis make neither method optimal in separating complex mixtures for downstream analysis 1 . Liquid and solid gelatinous materials have also been integrated in microchip-based *Correspondence should be addressed to Jongyoon Han [J. Han (email address: jyhan@mit.edu, Tel: 617-253-2290, Fax: 617-258-5846) systems for rapid separation of biomolecules (e.g., DNA, proteins and carbohydrates) with high resolution 5-7 . However, the foreign sieving matrices pose intrinsic difficulties for the integration of automated multi-step bioanalysis microsystems. Furthermore, these microchipbased systems are limited to analytical separation of biomolecules, due to the difficulty of harvesting purified biomolecules for downstream analysis.Recently, there has been great interest in switching from disordered porous gel media to patterned regular sieving structures, either by colloidal templating of self-assembled bead arrays 8,9 or by microfabrication techniques 10-15 . While significantly more efficient than gels in terms of separation speed and resolution, these regular sieving structures still largely resemble gels in the sense that separation is achieved by repeated sieving through multiple, identical "pores"....

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“…1c) 29,30 . The electric potential remains negative in the whole nanochannel, resulting in an electrostatic exclusion of negatively charged molecules.…”

Section: Design Of Anisotropic Nanofilter Arraymentioning

confidence: 99%

mentioning

confidence: 99%

A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

et al. 2007

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“…Latexes on porous silica or glass might seem to T1800, from (12) 461,000 257,000 1.8 T1800, from (15) 321,000 244,000 1.3 offer such a system, but we and others (6) since solute structure appears to be well defined, with charge groups residing at the distal ends of the molecule which a From supplier.…”

Section: Introductionmentioning

confidence: 86%

“…If the macro-charged rods into cavities of like charge (13). Smith and molecular species are similarly charged, repulsive effects Deen (14,15) provided a theoretical treatment for the perwill reduce their permeation. Several manifestations of this meation of spherical solutes (uniform surface charge, or effect may be noted.…”

Section: Introductionmentioning

confidence: 99%

“…ized by mercury intrusion. The materials prepared by Haller In this work we focus on the treatment of Smith and Deen typically had narrow pore size distributions (PSD) of 5- (14,15) in which the permeation coefficient of a charged 15% (22); commercial CPG may have larger PSDs but still sphere into a like-charged cylindrical cavity is computed as narrow compared to many semirigid gels. The pore structure a function of the sphere and cylinder radii, the cylindrical of CPG as seen by SEM has been compared to swiss cheese surface charge density, the ionic strength, and the sphere (23).…”

Section: Introductionmentioning

confidence: 99%

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Size-Exclusion Chromatography of Carboxyl-terminated Dendrimers as a Model for Permeation of Charged Particles into Like-Charged Cavities

Shah

Dubin

Kaplan

et al. 1996

Journal of Colloid and Interface Science

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Effect of ion binding on protein transport through ultrafiltration membranes

Menon

Zydney

1999

Biotech & Bioengineering

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Electrostatic interactions can have a significant impact on protein transmission through semipermeable membranes. Experimental data for the transport of bovine serum albumin (BSA) through a polyethersulfone ultrafiltration membrane were obtained in different salt solutions over a range of pH and salt concentrations. Net BSA charge under the same conditions was evaluated from mobility data measured by capillary electrophoresis. The results show that specific ionic composition, in addition to solution pH and ionic strength, can strongly affect the rate of protein transport through semipermeable ultrafiltration membranes. The effects of different ions on BSA sieving are due primarily to differences in ion binding to the protein, which leads to significant differences in the net protein charge at a given pH and ionic strength. This effect could be described in terms of an effective protein radius, which accounts for the electrostatic exclusion of the charged protein from the membrane pores. These results provide important insights into the nature of the electrostatic interactions in membrane systems. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 298–307, 1999.

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Electrostatic effects on the partitioning of spherical colloids between dilute bulk solution and cylindrical pores

Cited by 139 publications

References 14 publications

A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

Size-Exclusion Chromatography of Carboxyl-terminated Dendrimers as a Model for Permeation of Charged Particles into Like-Charged Cavities

Effect of ion binding on protein transport through ultrafiltration membranes

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