Electrospun vein grafts with high cell infiltration for vascular tissue engineering

Tan, Zhikai; Gao, Xiangkai; Liu, Tong; Yang, Yikun; Zhong, Juchang; Tong, Chunyi; Tan, Yongjun

doi:10.1016/j.msec.2017.08.034

Cited by 44 publications

(23 citation statements)

References 37 publications

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“…Electrospun fibrous membranes can also efficiently load bioactive agents and drugs for regulation of cellular physiological functions . However, ultrafine fibrous membranes may exhibit limitations by improving cell adhesion rather than cellular infiltration, whereas electrospun fibers in micron‐size can be of great benefit to cell ingrowth . Therefore, double or multiple‐layered electrospun membranes with different fiber diameters can fulfill various functions to facilitate rapid and complex tissue recovery .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, double or multiple‐layered electrospun membranes with different fiber diameters can fulfill various functions to facilitate rapid and complex tissue recovery . In vascular regeneration, bilayered or multilayered electrospun membranes in different diameters could meet diverse requirements of both VECs and VSMCs . The ultrafine fibrous membranes as the inner layer could be suitable for VEC adhesion and proliferation, and for the VSMC infiltration in the graft, the porosity of the outer layer in thick diameters is of importance …”

Section: Introductionmentioning

confidence: 99%

“…10,11 However, ultrafine fibrous membranes may exhibit limitations by improving cell adhesion rather than cellular infiltration, whereas electrospun fibers in micron-size can be of great benefit to cell ingrowth. [12][13][14][15] Therefore, double or multiple-layered electrospun membranes with different fiber diameters can fulfill various functions to facilitate rapid and complex tissue recovery. 12,13 In vascular regeneration, bilayered or multilayered electrospun membranes in different diameters could meet diverse requirements of both VECs and VSMCs.…”

Section: Introductionmentioning

confidence: 99%

“…12,13 In vascular regeneration, bilayered or multilayered electrospun membranes in different diameters could meet diverse requirements of both VECs and VSMCs. 6,14,15 The ultrafine fibrous membranes as the inner layer could be suitable for VEC adhesion and proliferation, and for the VSMC infiltration in the graft, the porosity of the outer layer in thick diameters is of importance. 6,14,15 MicroRNAs (miRNAs), that are small non-coding RNAs, can specifically bind to the target messenger RNAs and affect the synthesis process of proteins, and therapeutic interventions by miRNAs can regulate physiological behaviors of diverse cells.…”

Section: Introductionmentioning

confidence: 99%

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Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Cui

Wen

Zhou

et al. 2018

J Biomedical Materials Res

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Clinical utility of small‐diameter vascular grafts is still challenging in blood vessel regeneration owing to thrombosis and intimal hyperplasia. To cope with the issues, modulation of gene expression via microRNAs (miRNAs) could be a feasible approach by rational regulating physiological activities of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). Our previous studies demonstrated that individually loaded miRNA‐126 (miR‐126) or miRNA‐145 (miR‐145) in the electrospun membranes showed the tendency to promote vascular regeneration. In this work, the bilayered electrospun graft in 1.5‐mm diameter was developed by emulsion electrospinning to dual‐load miR‐126 and miR‐145 for target regulation of both VECs and VSMCs, respectively. Accelerated release of miR‐126 was achieved by introducing poly(ethylene glycol) in the inner electrospun poly(ethylene glycol)‐b‐poly(l‐lactide‐co‐caprolactone) ultrafine fibrous membrane, reaching 61.3 ± 1.2% of the cumulative release in the initial 10 days, whereas the outer electrospun poly(l‐lactide‐co‐glycolide) membrane composed of microfibers fulfilled prolonged release of miR‐145 for about 56 days. In vivo tests suggested that dual‐loading with miR‐126 and miR‐145 in the bilayered electrospun membranes could modulate both VECs and VSMCs for rapid endothelialization and hyperplasia inhibition as well. It is reasonably expected that dual target‐delivery of miR‐126 and miR‐145 in the electrospun vascular grafts has effective potential for small‐diameter vascular regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 371–382, 2019.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Cui

Wen

Zhou

et al. 2018

J Biomedical Materials Res

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“…Additionally, Tan and colleagues studied endothelial cell attachment to aligned PCL/PEG electrospun scaffolds and concluded that their bilayer grafts promoted cell adhesion and ECM production when fibers were oriented in the same direction 257 .…”

Section: Pcl After 30 Days In Rat Epididymal Fat (A) 4% Pcl Stained mentioning

confidence: 99%

Tissue engineered micro and macrovasculature utilizing stromal vascular fraction.

Zakhari¹

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Alignment behavior of nerve, vascular, muscle, and intestine cells in two‐ and three‐dimensional strategies

Jafari,

Behjat,

Malektaj

et al. 2023

WIREs Mechanisms of Disease

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By harnessing structural hierarchical insights, plausibly simulate better ones imagination to figure out the best choice of methods for reaching out the unprecedented developments of the tissue engineering products as a next level. Constructing a functional tissue that incorporates two‐dimensional (2D) or higher dimensions requires overcoming technological or biological limitations in order to orchestrate the structural compilation of one‐dimensional and 2D sheets (microstructures) simultaneously (in situ). This approach enables the creation of a layered structure that can be referred to as an ensemble of layers or, after several days of maturation, a direct or indirect joining of layers. Here, we have avoided providing a detailed methodological description of three‐dimensional and 2D strategies, except for a few interesting examples that highlight the higher alignment of cells and emphasize rarely remembered facts associated with vascular, peripheral nerve, muscle, and intestine tissues. The effective directionality of cells in conjunction with geometric cues (in the range of micrometers) is well known to affect a variety of cell behaviors. The curvature of a cell's environment is one of the factors that influence the formation of patterns within tissues. The text will cover cell types containing some level of stemness, which will be followed by their consequences for tissue formation. Other important considerations pertain to cytoskeleton traction forces, cell organelle positioning, and cell migration. An overview of cell alignment along with several pivotal molecular and cellular level concepts, such as mechanotransduction, chirality, and curvature of structure effects on cell alignments will be presented. The mechanotransduction term will be used here in the context of the sensing capability that cells show as a result of force‐induced changes either at the conformational or the organizational levels, a capability that allows us to modify cell fate by triggering downstream signaling pathways. A discussion of the cells' cytoskeleton and of the stress fibers involvement in altering the cell's circumferential constitution behavior (alignment) based on exposed scaffold radius will be provided. Curvatures with size similarities in the range of cell sizes cause the cell's behavior to act as if it was in an in vivo tissue environment. The revision of the literature, patents, and clinical trials performed for the present study shows that there is a clear need for translational research through the implementation of clinical trial platforms that address the tissue engineering possibilities raised in the current revision.This article is categorized under: Infectious Diseases > Biomedical Engineering Neurological Diseases > Biomedical Engineering Cardiovascular Diseases > Biomedical Engineering

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Electrospun vein grafts with high cell infiltration for vascular tissue engineering

Cited by 44 publications

References 37 publications

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Tissue engineered micro and macrovasculature utilizing stromal vascular fraction.

Alignment behavior of nerve, vascular, muscle, and intestine cells in two‐ and three‐dimensional strategies

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