Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds

Yuan, Jiang; Ghosh, Kaustabh; Shu, Xiao Zheng; Li, Bingquan; Sokolov, Jonathan; Prestwich, Glenn D.; Clark, Richard A.F.; Rafailovich, Miriam

doi:10.1016/j.biomaterials.2006.02.037

Cited by 353 publications

(214 citation statements)

References 40 publications

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“…SEM images showed that the cross-linked FDMA scaffold still maintained the 3D porous structure after PEO extraction. This structure is in agreement with the morphology of freeze-dried cross-linked fibers that were obtained via electrospinning of other polymeric materials (28). The porous structure was seen not only on the surface of the electrospun samples ( Fig.…”

Section: Resultssupporting

confidence: 75%

“…Such cross-linked fibers with encapsulated microorganisms can be used in various applications. However, FDMA cannot be cross-linked using conventional cross-linking approaches such as exposing the fibers to a waterbased cross-linking solution (28). Also, the nonvolatile nature of the cross-linking agent prevents the use of the vapor-phase crosslinking method (29)(30)(31).…”

Section: Resultsmentioning

confidence: 99%

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Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

Liu

Rafailovich

Malal

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Although microbes have been used in industrial and niche applications for several decades, successful immobilization of microbes while maintaining their usefulness for any desired application has been elusive. Such a functionally bioactive system has distinct advantages over conventional batch and continuous-flow microbial reactor systems that are used in various biotechnological processes. This article describes the use of polyethylene oxide 99-polypropylene oxide 67-polyethylene oxide99 triblock polymer fibers, created via electrospinning, to encapsulate microbes of 3 industrially relevant genera, namely, Pseudomonas, Zymomonas, and Escherichia. The presence of bacteria inside the fibers was confirmed by fluorescence microscopy and SEM. Although the electrospinning process typically uses harsh organic solvents and extreme conditions that generally are harmful to bacteria, we describe techniques that overcome these limitations. The encapsulated microbes were viable for several months, and their metabolic activity was not affected by immobilization; thus they could be used in various applications. Furthermore, we have engineered a microbe-encapsulated cross-linked fibrous polymeric material that is insoluble. Also, the microbe-encapsulated active matrix permits efficient exchange of nutrients and metabolic products between the microorganism and the environment. The present results demonstrate the potential of the electrospinning technique for the encapsulation and immobilization of bacteria in the form of a synthetic biofilm, while retaining their metabolic activity. This study has wide-ranging implications in the engineering and use of novel bio-hybrid materials or biological thin-film catalysts.biofilm ͉ cross-linking ͉ immobilization ͉ microbial ͉ Zymomonas

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

Liu

Rafailovich

Malal

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…To this end, nanofibrous scaffold will be preferred because of its similarity to ECM fibrils in both dimension and morphology. Indeed, the advantages of nanofibrous scaffold in promoting cell growth and maintaining proper phenotype have been demonstrated in a number of studies [5][6][7][8], which is a result of the synergistic effects of both nanotopography and chemical signalling [9,10]. Several approaches are available to fabricate nanofibres including self assembly [11], phase separation [12,5] and electrospinning [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional nanofibrous scaffold for tissue engineering

Yang

Ogbolu

Wang

2008

Journal of Experimental Nanoscience

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In tissue engineering, scaffolds with multiscale functionality, especially with the ability to release locally multiple or specific bioactive molecules to targeted cell types, are highly desired in regulating appropriate cell phenotypes. In this study, poly (epsilon-caprolactone) (PCL) solutions (8% w/v) containing different amounts of bovine serum albumin (BSA) with or without collagen were electrospun into nanofibres. As verified by protein release assay and fluorescent labelling, BSA and collagen were successfully incorporated into electrospun nanofibres. The biological activity of functionalised fibres was proven in the cell culture experiments using human dermal fibroblasts. By controlling the sequential deposition and fibre alignment, 3D scaffolds with spatial distribution of collagen or BSA were assembled using fluorescently labelled nanofibres. Human dermal fibroblasts showed preferential adhesion to PCL nanofibres containing collagen than PCL alone. Taken together, multiscale scaffolds with diverse functionality and tunable distribution of biomolecules across the nanofibrous scaffold can be fabricated using electrospun nanofibres.

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“…Aligned nanofibres support proper orientation of cell in scaffold and they also improve neurite outgrowth with proper differentiation of neural cell (28). The parameters such as viscosity, conductivity, surface tension of polymer solution, applied electric potential, flow rate, and distance between the electrodes are to be optimized while carrying on the electrospinning process (29).…”

Section: Electrospinning Technology For Nanofibres Fabrication:-mentioning

confidence: 99%