Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model

Zhi, Yunlong; Li, Wen; Zhang, Peng; Jiang, Jia; Chen, Shiyi

doi:10.1007/s10529-016-2158-4

Cited by 16 publications

(13 citation statements)

References 24 publications

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“…However, this animal model has been well established in the literatures. 27,46 Second, this is a pilot study with a small sample size, and the observation period and evaluation tools are limited. However, the differences are statistically significant among the groups, and the results are encouraging.…”

Section: Discussionmentioning

confidence: 99%

“…Immediately after sacrifice, the GTC samples (n = 5 in each group at each time point) were harvested and prepared for mechanical testing according to a previously reported procedure. 27 The peripheral tissues, except for the tendon graft, were carefully removed from the GTC. The biomechanical testing was performed using an electronic universal materials testing system machine (AGS-X; Shimadzu, Kyoto, Japan).…”

Section: Biomechanical Analysismentioning

confidence: 99%

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Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

Cai¹,

Wang²,

Ye³

et al. 2018

IJN

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BackgroundTendon/ligament injuries are common sports injuries. Clinically, the repair of a ruptured tendon or ligament to its bony insertion is needed, but the enthesis structure is not well reestablished following surgical repair. Herein, we fabricated dual-layer aligned-random scaffold (ARS) by electrospinning and aimed to investigate the effect of the scaffold on tendon-to-bone healing in vivo.Materials and methodsThe random and dual-layer aligned-random silk fbroin poly(L-lactic acid-co-e-caprolactone) (P(LLA-CL)) nanofibrous scaffolds were successfully fabricated by electrospinning methods. Ninety New Zealand white rabbits were randomly divided into three groups (random scaffold [RS], ARS, and control groups), and they were subjected to surgery to establish an extra-articular tendon-to-bone healing model with autologous Achilles tendon.ResultsHistological assessment showed that the ARS significantly increased the area of metachromasia, decreased the interface width, and improved collagen maturation and organization at the tendon–bone interface compared with the RS and control groups. Microcomputed tomography analysis showed that the bone tunnel area of RS and ARS groups was significantly smaller than those of the control group. Real-time polymerase chain reaction showed that BMP-2 and osteopontin expression levels of the tissue at the interface between the bone and graft in the RS and ARS groups were higher than those of the control group at 6 weeks. Collagen I expression level of the ARS group was significantly higher than those of the RS and control groups at 6 and 12 weeks. Moreover, the ARS groups had a better ultimate load-to-failure and stiffness than the RS and control groups.ConclusionARS could effectively augment the tendon-to-bone integration and improve gradient microstructure in a rabbit extra-articular model by inducing the new bone formation, increasing the area of fibrocartilage, and improving collagen organization and maturation. The dual-layer aligned-random silk fibroin/P(LLA-CL) nanofibrous scaffold is proved to be a promising biomaterial for tendon-to-bone healing.

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Section: Discussionmentioning

confidence: 99%

Section: Biomechanical Analysismentioning

confidence: 99%

Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

Cai¹,

Wang²,

Ye³

et al. 2018

IJN

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“…Despite the attraction offered by its biocompatibility, the main drawbacks of collagen scaffolds lie in their unsuitable mechanical properties linked to rapid degradation kinetics and poor structural stability as well as potentially their immunogenic character due to animal origin [320]. Alternative materials have been proposed for tendon reconstruction such as silk, which is a fibrous material secreted by spiders and by the caterpillars of certain butterflies (caterpillar of the mulberry bombyx) [321,322]. Silk possesses exceptional mechanical properties in terms of strength, toughness, and elasticity, making it popular in the field of tendon tissue engineering [323].…”

Section: Biomaterialsmentioning

confidence: 99%

In Vitro Innovation of Tendon Tissue Engineering Strategies

Citeroni

Ciardulli

Russo

et al. 2020

IJMS

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Tendinopathy is the term used to refer to tendon disorders. Spontaneous adult tendon healing results in scar tissue formation and fibrosis with suboptimal biomechanical properties, often resulting in poor and painful mobility. The biomechanical properties of the tissue are negatively affected. Adult tendons have a limited natural healing capacity, and often respond poorly to current treatments that frequently are focused on exercise, drug delivery, and surgical procedures. Therefore, it is of great importance to identify key molecular and cellular processes involved in the progression of tendinopathies to develop effective therapeutic strategies and drive the tissue toward regeneration. To treat tendon diseases and support tendon regeneration, cell-based therapy as well as tissue engineering approaches are considered options, though none can yet be considered conclusive in their reproduction of a safe and successful long-term solution for full microarchitecture and biomechanical tissue recovery. In vitro differentiation techniques are not yet fully validated. This review aims to compare different available tendon in vitro differentiation strategies to clarify the state of art regarding the differentiation process.

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“…After 12 weeks, they found increased bone regeneration. Moreover, the electrospun mats could not be pulled out from the bones and showed a statistically significant increment of the mechanical properties compared to the control groups [ 129 ].…”

Section: Applications For Tendon and Ligament Regeneration And Repmentioning

confidence: 99%

Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments

2018

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Tendon and ligament tissue regeneration and replacement are complex since scaffolds need to guarantee an adequate hierarchical structured morphology, and non-linear mechanical properties. Moreover, to guide the cells’ proliferation and tissue re-growth, scaffolds must provide a fibrous texture mimicking the typical of the arrangement of the collagen in the extracellular matrix of these tissues. Among the different techniques to produce scaffolds, electrospinning is one of the most promising, thanks to its ability to produce fibers of nanometric size. This manuscript aims to provide an overview to researchers approaching the field of repair and regeneration of tendons and ligaments. To clarify the general requirements of electrospun scaffolds, the first part of this manuscript presents a general overview concerning tendons’ and ligaments’ structure and mechanical properties. The different types of polymers, blends and particles most frequently used for tendon and ligament tissue engineering are summarized. Furthermore, the focus of the review is on describing the different possible electrospinning setups and processes to obtain different nanofibrous structures, such as mats, bundles, yarns and more complex hierarchical assemblies. Finally, an overview concerning how these technologies are exploited to produce electrospun scaffolds for tendon and ligament tissue applications is reported together with the main findings and outcomes.

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Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model

Abstract: The electrospun SF mat wrapping could enhance tendon-bone healing of soft tissue graft.

Cited by 16 publications

References 24 publications

Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

In Vitro Innovation of Tendon Tissue Engineering Strategies

Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments

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