Electrospun scaffolds for stem cell engineering

Lim, Shawn; Mao, Hai‐Quan

doi:10.1016/j.addr.2009.07.011

Cited by 278 publications

(205 citation statements)

References 67 publications

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“…The electrospun scaffolds are formed by fibers that are able to mimic in structure and scale the collagen fibers of the native extracellular matrix, providing a favorable and biomimetic microenvironment for cell adhesion, spreading, and development. 1,2 The efficacy of the use of scaffolds depends on their capacity to interact with cells. The interaction between the cells and scaffolds begins with the seeding process.…”

Section: Introductionmentioning

confidence: 99%

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Braghirolli¹,

Zamboni²,

Acasigua³

et al. 2015

IJN

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In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide- co -glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO 2 . The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Braghirolli¹,

Zamboni²,

Acasigua³

et al. 2015

IJN

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“…The current used technique, electrospinning, is an advantageous method for fabrication of scaffolds where the major physical, chemical and mechanical properties of the ECM can be replicated and modified [39].…”

Section: Discussionmentioning

confidence: 99%

Tailor-Made Electrospun Culture Scaffolds Control Human Neural Progenitor Cell Behavior—Studies on Cellular Migration and Phenotypic Differentiation

Englund-Johansson¹,

Netanyah²,

Johansson³

2017

JBNB

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In neuroscience research, cell culture systems are essential experimental platforms. It is of great interest to explore in vivo-like culture substrates. We explored how basic properties of neural cells, nuclei polarization, phenotypic differentiation and distribution/migration, were affected by the culture at poly-L-lactic acid (PLLA) fibrous scaffolds, using a multipotent mitogen-expanded human neural progenitor cell (HNPC) line. HNPCs were seeded, at four different surfaces: two different electrospun PLLA (d = 1.2 -1.3 µm) substrates (parallel or random aligned fibers), and planar PLL-and PLLA surfaces. Nuclei analysis demonstrated a non-directed cellular migration at planar surfaces and random fibers, different from cultures at aligned fibers where HNPCs were oriented parallel with the fibers. At aligned fibers, HNPCs displayed the same capacity for phenotypic differentiation as after culture on the planar surfaces. However, at random fibers, HNPCs showed a significant lower level of phenotypic differentiation compared with cultures at the planar surfaces. A clear trend towards greater neuronal formation at aligned fibers, compared to cultures at random fibers, was noted. We demonstrated that the topography of in vivo-resembling PLLA scaffolds significantly influences HNPC behavior, proven by different migration behavior, phenotypic differentiation potential and nuclei polarization.This knowledge is useful in future exploration of in vivo-resembling neural cell system using electrospun scaffolds.

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“…Numerous efforts have been made to explore the biocompatibility of the electrospun fibrous scaffolds through the study of cell culture [9][10][11][12][13] . The confocal microscopy images in Figure 4 show that the attached cells with the strong green fluorescent signals indicated the cell survival on PGD fibers.…”

Section: Discussionmentioning

confidence: 99%

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Dai

Huang

2014

JoVE

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For tissue engineering applications, the preparation of biodegradable and biocompatible scaffolds is the most desirable but challenging task. Among the various fabrication methods, electrospinning is the most attractive one due to its simplicity and versatility. Additionally, electrospun nanofibers mimic the size of natural extracellular matrix ensuring additional support for cell survival and growth. This study showed the viability of the fabrication of long fibers spanning a larger deposit area for a novel biodegradable and biocompatible polymer named poly(glyceroldodecanoate) (PGD) 1 by using a newly designed collector for electrospinning. PGD exhibits unique elastic properties with similar mechanical properties to nerve tissues, thus it is suitable for neural tissue engineering applications. The synthesis and fabrication set-up for making fibrous scaffolding materials was simple, highly reproducible, and inexpensive. In biocompatibility testing, cells derived from mouse embryonic stem cells could adhere to and grow on the electrospun PGD fibers. In summary, this protocol provided a versatile fabrication method for making PGD electrospun fibers to support the growth of mouse embryonic stem cell derived neural lineage cells.

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Electrospun scaffolds for stem cell engineering

Cited by 278 publications

References 67 publications

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Tailor-Made Electrospun Culture Scaffolds Control Human Neural Progenitor Cell Behavior—Studies on Cellular Migration and Phenotypic Differentiation

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

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