Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Dai, Xizi; Huang, Yun-Chen

doi:10.3791/51587

Cited by 5 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PGD was synthesized by a recipe modified from the previously reported one. ,− In details, dodecanedioic acid (DDA, 99%, Alfa Aesar) and glycerol (>99%, synthetic, ACROS Organics) were purchased from Thermo Fisher Scientific and used without further purification. They were weighed with a 1:1 molar ratio and put into a three-necked flask for reaction.…”

Section: Methodsmentioning

confidence: 99%

Chemically Interconnected Thermotropic Polymers for Transparency-Tunable and Impact-Resistant Windows

Zhang

Deng

Kenderes

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Thermotropic polymers with the capability of thermally tuning transparency are widely applied in smart windows and energy-saving windows, playing a critical role in enhancing comfort level and energy efficiency of indoor spaces. Usually, thermotropic polymer systems are constructed by physically dispersing phase transition materials in transparent hosting materials. However, bad interfaces universally exist in these systems, resulting in poor mechanical properties, weak interfaces to substrates, or bad long-term stability. Herein, we demonstrate a novel chemically interconnected thermotropic polymer, which is obtained by reacting dodecanedioic acid (DDA) with glycerol. In the system, some of DDA molecules were cross-linked to form a polyester network, poly(glycerol-dodecanoate) (PGD). Other grafted but non-cross-linked DDA molecules form semicrystalline domains, which possess a solid–liquid phase transition within the PGD matrix. The phase transition offers the resulting hybrid materials with tunable optical transparency. The PGD–DDA system shows stable performance after 100 heating–cooling cycles. In addition, when applied for window coating, it results in tough interfacial bonding to glass substrates with toughness of >6910 J m–2 below its transition temperature and >135 J m–2 above its transition temperature. It increases the impact resistance of the window by multiple times.

show abstract

Section: Methodsmentioning

confidence: 99%

Chemically Interconnected Thermotropic Polymers for Transparency-Tunable and Impact-Resistant Windows

Zhang

Deng

Kenderes

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…After gluing the edges of the scaffolds to the underlying glass, coverslips were placed in six-well culture plates. Before introducing cell cultures, the wells containing the coverslips were UV-sterilized and immersed in 1Â phosphate-buffered saline (PBS) at 37 C in an incubator overnight (43).…”

Section: Fabrication Of Electrospun Scaffoldsmentioning

confidence: 99%

Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment

Dagro

Rajbhandari

Orrego

et al. 2019

Biophysical Journal

View full text Add to dashboard Cite

Measurements of the mechanical response of biological cells are critical for understanding injury and disease, for developing diagnostic tools, and for computational models in mechanobiology. Although it is well known that cells are sensitive to the topography of their microenvironment, the current paradigm in mechanical testing of adherent cells is mostly limited to specimens grown on flat two-dimensional substrates. In this study, we introduce a technique in which cellular indentation via optical trapping is performed on cells at a high spatial resolution to obtain their regional mechanical properties while they exist in a more favorable three-dimensional microenvironment. We combine our approach with nonlinear contact mechanics theory to consider the effects of a large deformation. This allows us to probe length scales that are relevant for obtaining overall cell stiffness values. The experimental results herein provide the hyperelastic material properties at both high ($100 s À1 ) and low ($1-10 s À1 ) strain rates of murine central nervous system glial cells. The limitations due to possible misalignment of the indenter in the three-dimensional space are examined using a computational model.

show abstract

“…In addition to the original formulation of PGD previously reported, electrospun mats of PGD, PGD modified with fumaric acid (PGDF), or gelatin‐blended PGD have been explored for neural tissue engineering. It is presently unclear, however, if the mechanical properties of PGD can be altered to produce shape memory materials with a range of tangent stiffness values to accommodate use within multiple soft tissues.…”

Section: Introductionmentioning

confidence: 99%

Tailoring the physicochemical and shape memory properties of the biodegradable polymer poly(glycerol dodecanoate) via curing conditions

Solorio

Bocks

Hollister

2017

J Biomedical Materials Res

View full text Add to dashboard Cite

A major challenge in the repair and regeneration of soft tissue damage occurring as a result of aging, injury, or disease is recapitulating the biomechanical properties of the native tissue. Ideally, a candidate biomaterial for soft tissue engineering applications should be biocompatible, nonlinearly elastic to match soft tissue mechanical behavior, biodegradable to enable tissue remodeling, and tailorable to achieve a range of nonlinear elastic mechanical properties to match specific soft tissues. In addition, for cardiac and other applications, the biomaterial should have shape memory characteristics to facilitate minimally invasive and/or catheter-based delivery. Poly(glycerol dodecanoate) (PGD) is a shape memory material that has nonlinear elastic properties at body temperature and elastic-plastic behavior at room temperature. In this study, we investigated the effects of curing conditions on the nonlinear elastic, shape memory, and biocompatibility properties of PGD. Increased curing and crosslinking resulted in an increase in both the initial stiffness and the nonlinear strain stiffening behavior of PGD. After shape fixation at 60% strain, 100% shape recovery was achieved within 1 min at body temperature for all conditions tested. Polymer curing had no adverse effects on the cellular biocompatibility or non-hemolytic characteristics of PGD, indicating the potential suitability of these formulations for blood-contacting device applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1618-1623, 2017.

show abstract

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Cited by 5 publications

References 13 publications

Chemically Interconnected Thermotropic Polymers for Transparency-Tunable and Impact-Resistant Windows

Chemically Interconnected Thermotropic Polymers for Transparency-Tunable and Impact-Resistant Windows

Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment

Tailoring the physicochemical and shape memory properties of the biodegradable polymer poly(glycerol dodecanoate) via curing conditions

Contact Info

Product

Resources

About