Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO2 and Na2Ti6O13 Particles for Potential Use in Bone Regeneration

Ramírez-Cedillo, Erick; Ortega-Lara, Wendy; Rocha-Pizaña, María del Refugio; Gutiérrez-Uribe, Janet A.; Elı́as-Zúñiga, Alex; Rodrı́guez, Ciro A.

doi:10.3390/membranes9010012

Cited by 30 publications

(18 citation statements)

References 51 publications

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“…As shown in Figure , the fiber diameters changes between 700 and 900 nm, and prepared mats exhibit the pore size of more than 7 μm. Because of the high area surface to volume ratio of nanofibers, the spread and attachment of cells improve in comparison with microfibers, and the pore size of more than 7 μm provides a suitable condition for the osteoblast cell growth and proliferation . As seen in Figure and Table , the diameter of fibers decreases by increasing the nanoceramic concentration, since the dielectric properties of the nanocomposite solution have been enhanced and the solution conductivity has affected the morphology of fibers .…”

Section: Resultsmentioning

confidence: 99%

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Johari

Fathi

Fereshteh

et al. 2019

Polymers for Advanced Techs

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Biodegradable cell-incorporated scaffolds can guide the regeneration process of bone defects such as physiological resorption, tooth loss, and trauma which medically, socially, and economically hurt patients. Here, 0, 5, 10, and 15 wt% fluoridated hydroxyapatite (FHA) nanoparticles containing 25 wt% F − and 75 wt% OH − were incorporated into poly (ε-caprolactone) (PCL) matrix to produce PCL/FHA nanocomposite scaffolds using electrospinning method. Then, scanning electron microscopy (SEM), X-ray diffraction (XRD) pattern, and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the morphology, phase structure, and functional groups of prepared electrospun scaffolds, respectively. Furthermore, the tensile strength and elastic modulus of electrospun scaffolds were investigated using the tensile test. Moreover, the biodegradation behavior of electrospun PCL/FHA scaffolds was studied by the evaluation of weight loss of mats and the alternation of pH in phosphate buffer saline (PBS) up to 30 days of incubation.Then, the biocompatibility of prepared mats was investigated by culturing MG-63 osteoblast cell line and performing MTT assay. In addition, the adhesion of osteoblast cells on prepared electrospun scaffolds was studied using their SEM images. Results revealed that the fiber diameter of prepared electrospun PCL/FHA scaffolds alters between 700 and 900 nm. The mechanical assay illustrated the mat with 10 wt% FHA nanoparticles revealed the highest tensile strength and elastic modulus. The weight loss alternation of mats determined around 1% to 8% after 30 days of incubation. The biocompatibility and cell adhesion of mats improved by increasing the amounts of FHA nanoparticles. K E Y W O R D S biocompatibility, electrospinning, nano-fluoridated hydroxyapatite, poly(ɛ-caprolactone), tensile strength 1 | INTRODUCTION Fibrous structure scaffolds exhibits the similar size to the extracellular matrix structure, protein absorption, and cell attachment in tissue engineering applications. 1 As an effective and low-cost fabricating method, electrospinning has been applied to produce continuous fibers from the nanoscale to the microscale. 2 Tissue engineering scaffolds should essentially possess several properties such as desirable biocompatibility, simple fabrication, controllable degradation rate, and sufficient mechanical properties. Accordingly, diverse synthetic polymers have been electrospun and modified as well as natural polymersto perform in different tissues. 3 Among these, poly(ε-caprolactone) (PCL) as a synthetic and biodegradable polymer possesses the biocompatibility and rheological and viscoelastic properties, prepared as a fibrous structure via the electrospinning. 4-6 Hence, PCL fibers can be selected as an appropriate candidate for tissue engineering scaffolds, regenerating skin, cartilage, bone, and cardiac constructs. 7 PCL is a polyester with a semicrystalline structure and amorphous domains,

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Section: Resultsmentioning

confidence: 99%

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Johari

Fathi

Fereshteh

et al. 2019

Polymers for Advanced Techs

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“…On the other hand, some studies confirm that PCL fibers without any treatment do not provoke a considerable inhibition of S. aureus [95,101]. Adeli-Sardou M. et al (2018), reported that PCL/GEL fiber did not affect biofilm-producing bacteria S. aureus, P. mirabilis, MRSA and P. aeruginosa [102]. However, PCL has been used as a base material to fabricate antimicrobial nanofibers, such as the case of PCL 10% loaded with organic modified-montmorillonite and curcumin, which revealed high antimicrobial activity [103].…”

Section: Discussionmentioning

confidence: 99%

Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings

et al. 2020

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Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit™ silver nanoparticles (Ag-Si/Al2O3) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58–60 °C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria Pseudomona aeruginosa and Escherichia coli, Gram-positive Staphylococcus aureus and the fungus Candida albicans. The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-Al2O3-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-Al2O3-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity.

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“…The utilization of bioactive ceramic materials such as TiO 2 and Na 2 Ti 6 O 13 in the electrospinning method for the preparation of PCL nanocomposites membranes with plausible bioactivity from which the bone tissue regeneration can be established is an area of interest in medical society. Due to their abundance, insignificant toxicity and stable chemical structure, ceramic particles can be treated by the electrospinning technique to allow the formation of the membranes with viable stiffness and bioactive characteristics for cell proliferation and excellent production of bone structures [34,36].…”

Section: Electrospun Nanoparticlesmentioning

confidence: 99%

Morphology and Properties of Electrospun PCL and Its Composites for Medical Applications: A Mini Review

et al. 2019

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Polycaprolactone (PCL) is one of the most used synthetic polymers for medical applications due to its biocompatibility and slow biodegradation character. Combining the inherent properties of the PCL matrix with the characteristic of nanofibrous particles, result into promising materials that can be suitable for different applications, including the biomedical applications. The advantages of nanofibrous structures include large surface area, a small diameter of pores and a high porosity, which make them of great interest in different applications. Electrospinning, as technique, has been heavily used for the preparation of nano- and micro-sized fibers. This review discusses the different methods for the electrospinning of PCL and its composites for advanced applications. Furthermore, the steady state conditions as well as the effect of the electrospinning parameters on the resultant morphology of the electrospun fiber are also reported.

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Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO2 and Na2Ti6O13 Particles for Potential Use in Bone Regeneration

Cited by 30 publications

References 51 publications

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings

Morphology and Properties of Electrospun PCL and Its Composites for Medical Applications: A Mini Review

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