Electrospun poly(l-lactic acid) fiber mats containing crude Garcinia mangostana extracts for use as wound dressings

Suwantong, Orawan; Pankongadisak, Porntipa; Deachathai, Suwanna; Supaphol, Pitt

doi:10.1007/s00289-014-1102-9

Cited by 33 publications

(26 citation statements)

References 52 publications

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“…This dehydrogenase reagent indicator (tetrazolium salt) works based on the reduction reaction of yellow tetrazolium salt to purple formazan crystals by the action of dehydrogenase enzymes on the mitochondria of the cell (Fig. 3) [14]. The succinic dehydrogenase produced by the mitochondria of living cells and contained within these microbes can actively convert the tetrazolium (MTT) salt into purple formazan [10].…”

Section: Resultsmentioning

confidence: 99%

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

Ambarwati

Malik²,

Deborah³

et al. 2017

Asian J Pharm Clin Res

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Objective: This research focuses on the antibacterial activities of fractions of ethyl acetate Garcinia latissima Miq. extracts against Bacillus subtilis and Pseudomonas aeruginosa. Methods:For each bacteria, the values of the inhibitory zone diameter were determined using paper discs method, while the minimum inhibitory concentration (MIC) was determined by microdilution and antibacterial assay was determined by bioautography. Fractionation was carried out using gradient elution with silica gel column as stationary phase successively increasing the separation according to the polarity (using combination of eluents; n-hexane, ethyl acetate, and methanol). Results:To determine the diameter of the inhibitory zone for each fraction, the fractions were diluted with dimethyl sulfoxide until the concentration of each fraction was 20,000 ppm. The results showed that fraction B had no inhibitory zone against B. subtilis. The inhibitory zone diameters of fractions A, C, D, E, F, and G against B. subtilis were 7.600±0.000, 6.767±0.202, 7.950±0.477, 7.883±0.901, and 9.233±0.231 mm, respectively. Only Fraction G had an inhibition zone diameter of 7.200±0.173 mm against P. aeruginosa. The active fractions were tested by contact bioautography using silica gel (60 GF254) plate and hexane:chloroform (2:3) as mobile phase for Fractions A, C, D, E, and F and chloroform:ethyl acetate (4:1) as mobile phase for Fraction G. The thin layer chromatography plates were observed under the ultraviolet light at wavelengths of 254 and 366 nm, while the others plate (using in contact bioautography) placing on agar medium that had been inoculated with the bacteria, so the compounds could be diffuse to the medium. The active compound will appeared inhibitory zones in agar medium. The determination of the MIC values using microdilution with methylthiazol tetrazolium salt showed that fractions of the ethyl acetate extracts of G. latissima Miq. stem bark inhibited the growth of B. subtilis more actively than P. aeruginosa. The highest activity of the fractions against B. subtilis was shown by fraction G, which had a MIC value of 78.125 ppm, followed by fractions F (156.25 ppm), E (312.5 ppm), D (625 ppm), C (1250 ppm), B (2500 ppm), and A (5000 ppm). The highest activity of the fractions against P. aeruginosa was Fraction G (5000 ppm), while the MIC values of other fractions were >5000 ppm. Conclusion:Further investigations should be conducted to obtain the new antibacterial compounds in fractions of ethyl acetate extract of G. latissima Miq. stem bark that effective to inhibit the growth of B. subtilis and P. aeruginosa.

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Section: Resultsmentioning

confidence: 99%

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

Ambarwati

Malik²,

Deborah³

et al. 2017

Asian J Pharm Clin Res

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“…Based on DPPH (2,2diphenyl-1-picrylhydrazyl) assays, a variety of phenolic compounds obtained from the flower, fruit, and seed parts exhibited radical scavenging activity. Ten µM each of quercetin 3-O-βgalactopyranoside (30), fukugeside (32), xanthochymol (34), and guttiferone E (37) from the flowers showed potent antioxidant activity producing scavenging values of 57%, 56%, 60% and 59%, respectively, whereas that of the reference anti-oxidant, butylated hydroxytoluene (BHT) was only 43% [21]. Among the 42 compounds isolated from the green and ripe fruits, morelloflavone (1), camboginol (52), dulcisflavan (69), cambogin (79), and (-)epicathechin (81) were effective scavengers of the DPPH radical.…”

Section: Anti-oxidant Activitymentioning

confidence: 99%

“…Recently, Suwantong and co-workers have demonstrated by the disc diffusion assay that electrospun poly(L-lactic acid) (PPLA) fiber mats containing an acetone extract of G. dulcis flowers were active against some common pathogenic microorganisms found on burn wounds with the highest potency against Streptococcus pyogenes, followed by P. aeruginosa, S. aureus, S. epidermidis, Acinetobacter calcoaceticus, Streptococcus agalactiae, and Candida albicans. In addition, they were non-toxic to the normal human fibroblasts making their potential for use as wound dressings [30]. Among 15 different compounds isolated from the fruits of G. dulcis that were evaluated for their anti-bacterial activity (1, 33, 35-36, 39, 42-43, 51-53, 57, 60, 68, 79-80), only cowaxanthone (33), -mangostin (39), camboginol (52), and lupabigenin (60) could inhibit S. aureus and MRSA but in moderate potency with MICs ranging from 4-16 µg/mL as compared with vancomycin (MICs 2 µg/mL), whereas the others showed either weak or no activity [23].…”

Section: Anti-bacterial Activitymentioning

confidence: 99%

Phytoconstituents and Biological Activities ofGarcinia Dulcis(Clusiaceae): A Review

Khamthong

Hutadilok‐Towatana

2017

Natural Product Communications

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Garcinia dulcis (Roxb.) Kurz is a tropical fruit tree native to Southeast Asia where it has a long history of use as a traditional medicine for the treatment of ailments such as lymphatitis, parotitis, struma, scurvy, cough, and sore throat. Despite its medicinal values, this plant is not well known and rarely found nowadays. Research on the phytochemical constituents and biological activities of G. dulcis have demonstrated that various parts of the plant contain an abundance of bioactive compounds mainly xanthones and flavonoids, with significant pharmacological properties such as anti-atherosclerosis, anti-bacterial, anti-cancer, anti-hypertension, and anti-malarial. In the present review, current knowledge of the phytochemistry of G. dulcis and biological activities of its active constituents based on the available literature are summarized in order to explore application potentials and prospective research works on this plant.

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“…Kurz, commonly known as "Ma Phut" in Thailand, or Mundu in Indonesia and Malaysia, is in the Guttiferae family and is also found cultivated as a home garden plant in Thailand and other Southeast Asian countries. Garcinia dulcis (G. dulcis) is one of the most important species of genus Garcinia, which are a rich source of aromatic metabolites, such as flavonoids, benzophenones, and xanthones (Suwantong et al 2013). The traditional ethnomedical uses of G. dulcis are as follows.…”

Section: Introductionmentioning

confidence: 99%

Eco-Dyeing of Silk Fabric withGarcinia Dulcis(Roxb.) Kurz Bark as a Source of Natural Dye by using The Padding Technique

Mongkholrattanasit

Saiwan

Rungruangkitkrai

et al. 2015

Journal of Natural Fibers

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Silk fabrics were put through a process of dyeing with natural colorants extracted and used from the bark of the Garcinia dulcis (Roxb.) Kurz. Two padding techniques, pad-batch and pad-dry, were used under different study conditions. The effects of mordant agents used on the fabrics and the natural colorant in the dyeing process, color values, color strength, and the fastness properties of silk fabric were investigated and the results recorded in the following study. The results showed that color values can be changed using various metallic salts as mordants. The fastness properties ranged from fair to very good, while perspiration and light fastness was poor to fair.

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Electrospun poly(l-lactic acid) fiber mats containing crude Garcinia mangostana extracts for use as wound dressings

Cited by 33 publications

References 52 publications

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

Phytoconstituents and Biological Activities ofGarcinia Dulcis(Clusiaceae): A Review

Eco-Dyeing of Silk Fabric withGarcinia Dulcis(Roxb.) Kurz Bark as a Source of Natural Dye by using The Padding Technique

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