Electrospun poly(l-lactic acid-co-ɛ-caprolactone) fibers loaded with heparin and vascular endothelial growth factor to improve blood compatibility and endothelial progenitor cell proliferation

Chen, Xi; Wang, Jing; An, Qingzhu; Li, Dawei; Liu, Peixi; Zhu, Wei; Mo, Xiumei

doi:10.1016/j.colsurfb.2015.02.023

Cited by 94 publications

(69 citation statements)

References 48 publications

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“…These include modifications such as heparin-coated scaffolds, VEGF, antibodies, and various peptides. 28,34,38,46 Such modifications may improve initial cell seeding, which, in turn, may expedite in vitro endothelialization and tissue formation on bioreactor-cultured TEVGs. In addition, there is still some controversy on the role of EPCs and the role of specific subsets of these cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

Melchiorri

Bracaglia

Kimerer

et al. 2016

Tissue Engineering Part C: Methods

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Section: Discussionmentioning

confidence: 99%

In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

Melchiorri

Bracaglia

Kimerer

et al. 2016

Tissue Engineering Part C: Methods

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“…However, the results are conflicting due to the recruitment of other positive cells, which accelerate the formation of thrombus and the occurrence of inflammatory response . Secondly, cytokines, like nerve growth factor (NGF), stromal cell‐derived factor‐1

normalα

, brain‐derived neurotrophic factor, and VEGF are used to bind small‐diameter vascular grafts to recruit EPCs . Moreover, peptides such as TPS, RGD, and YIGSR were applied to cross‐link with small‐diameter vascular grafts to capture EPCs .…”

Section: Unipotent Stem Cellsmentioning

confidence: 99%

“…13 Secondly, cytokines, like nerve growth factor (NGF), stromal cellderived factor-1a, brain-derived neurotrophic factor, and VEGF are used to bind small-diameter vascular grafts to recruit EPCs. 14,[148][149][150] Moreover, peptides such as TPS, RGD, and YIGSR were applied to cross-link with small-diameter vascular grafts to capture EPCs. [151][152][153] Other modification methods, such as polymer cross-linking and coating with specific EPC capturing aptamers, have also been reported.…”

Section: Unipotent Stem Cellsmentioning

confidence: 99%

Cell‐based strategies for vascular regeneration

Zou

Fan

Fartash

et al. 2016

J Biomedical Materials Res

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Vascular regeneration is known to play an essential role in the repair of injured tissues mainly through accelerating the repair of vascular injury caused by vascular diseases, as well as the recovery of ischemic tissues. However, the clinical vascular regeneration is still challenging. Cell-based therapy is thought to be a promising strategy for vascular regeneration, since various cells have been identified to exert important influences on the process of vascular regeneration such as the enhanced endothelium formation on the surface of vascular grafts, and the induction of vessel-like network formation in the ischemic tissues. Here are a vast number of diverse cell-based strategies that have been extensively studied in vascular regeneration. These strategies can be further classified into three main categories, including cell transplantation, construction of tissue-engineered grafts, and surface modification of scaffolds. Cells used in these strategies mainly refer to terminally differentiated vascular cells, pluripotent stem cells, multipotent stem cells, and unipotent stem cells. The aim of this review is to summarize the reported research advances on the application of various cells for vascular regeneration, yielding insights into future clinical treatment for injured tissue/organ.

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“…Chen et al reported encapsulating heparin into the core of PLCL core-shell nanofibers via emulsion electrospinning to fabricate a nanofiber scaffold for anticoagulation [23]. Jiang et al demonstrated that modification of decellularized vascular grafts with elastomer and heparin resulted in significant improvement in antithrombotic properties [24].…”

Section: Commentmentioning

confidence: 99%

Novel Bioresorbable Vascular Graft With Sponge-Type Scaffold as a Small-Diameter Arterial Graft

Sugiura

Tara

Nakayama

et al. 2016

The Annals of Thoracic Surgery

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Background Current commercialized small-diameter arterial grafts have not shown clinical effectiveness due to their poor patency rates. The purpose of the present study is to evaluate the feasibility of arterial bioresorbable vascular graft, which has a porous sponge type scaffold, as a small-diameter arterial conduit. Methods The grafts were constructed by a 50:50 poly (l-lactic-co-ε-caprolactone) copolymer (PLCL) scaffold reinforced by a poly (l-lactic acid) (PLA) nano-fiber. The pore size of the PLCL scaffold was adjusted to a small size (12.8 ± 1.85 μm) or a large size (28.5 ± 5.25 μm). We compared the difference in cellular infiltration followed by tissue remodeling between the groups. The grafts were implanted in 8–10 week old female mice (n = 15 in each group) as infra-renal aortic interposition conduits. Animals were followed for 8 weeks and sacrificed to evaluate neotissue formation. Results No aneurysmal change or graft rupture was observed in both groups. Histologic assessment demonstrated favorable cell infiltration into scaffolds, neointimal formation with endothelialization, smooth muscle cell proliferation, and elastin deposition in both groups. No significant difference was observed between the groups. Immunohistochemical characterization with anti-F4/80 antibody demonstrated that macrophage infiltration into the grafts occurred in both groups. Staining for M1 and M2, which are the two major macrophage phenotypes, showed no significant difference between groups. Conclusions Our novel bioresorbable vascular grafts showed well-organized neointimal formation in the high pressure arterial circulation environment. Whereas the large pore size scaffold did not improve cellular infiltration and neotissue formation when compared to the small pore scaffold.

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Electrospun poly(l-lactic acid-co-ɛ-caprolactone) fibers loaded with heparin and vascular endothelial growth factor to improve blood compatibility and endothelial progenitor cell proliferation

Cited by 94 publications

References 48 publications

In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

Cell‐based strategies for vascular regeneration

Novel Bioresorbable Vascular Graft With Sponge-Type Scaffold as a Small-Diameter Arterial Graft

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