Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Yu, Jihong; Lee, An-Rei; Lin, Wei-Yang; Lin, Chan-Shing; Wu, Yen Wen; Tsai, Wei‐Bor

doi:10.1089/ten.tea.2013.0008

Cited by 99 publications

(69 citation statements)

References 43 publications

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“…12 Meanwhile, the functional molecules (eg, drugs and growth factors) and bioactive elements can also be electrospun into the composite nanofibers to achieve high bioactivity and cell affinity. [13][14][15] Nanostructured CaP materials with high biocompatibility are usually added in the polymer solution to modify the electrospun composite nanofibers. [16][17][18] For example, hydroxyapatite (HA) and anti-inflammatory drugs can be incorporated into polycaprolactone fibers by electrospinning, achieving sustained drug release and mineralization.…”

Section: Fu Et Almentioning

confidence: 99%

Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Fu¹,

Zi²,

Xu³

et al. 2016

IJN

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Calcium phosphate-based biomaterials have been well studied in biomedical fields due to their outstanding chemical and biological properties which are similar to the inorganic constituents in bone tissue. In this study, amorphous calcium phosphate (ACP) nanoparticles were prepared by a precipitation method, and used for preparation of ACP-poly( d , l -lactic acid) (ACP-PLA) nanofibers and water-soluble drug-containing ACP-PLA nanofibers by electrospinning. Promoting the encapsulation efficiency of water-soluble drugs in electrospun hydrophobic polymer nanofibers is a common problem due to the incompatibility between the water-soluble drug molecules and hydrophobic polymers solution. Herein, we used a native biomolecule of lecithin as a biocompatible surfactant to overcome this problem, and successfully prepared water-soluble drug-containing ACP-PLA nanofibers. The lecithin and ACP nanoparticles played important roles in stabilizing water-soluble drug in the electrospinning composite solution. The electrospun drug-containing ACP-PLA nanofibers exhibited fast mineralization in simulated body fluid. The ACP nanoparticles played the key role of seeds in the process of mineralization. Furthermore, the drug-containing ACP-PLA nanofibers exhibited sustained drug release which simultaneously occurred with the in situ mineralization in simulated body fluid. The osteoblast-like (MG63) cells with spreading filopodia were well observed on the as-prepared nanofibrous mats after culturing for 24 hours, indicating a high cytocompatibility. Due to the high biocompatibility, sustained drug release, and fast mineralization, the as-prepared composite nanofibers may have potential applications in water-soluble drug loading and release for tissue engineering.

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Section: Fu Et Almentioning

confidence: 99%

Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Fu¹,

Zi²,

Xu³

et al. 2016

IJN

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“…Two adhesive peptides N-acetyl-GRGDSPGYG (RGD) and N-acetyl-GYIGSRGYG (YIGSR) -both deriving from laminin -were mixed with PLGA to electrospin, and compared to laminincoated PLGA scaffolds [121]. In vitro studies using rat CMs indicated that the cells cultured on the YIGSR incorporated and the laminin-coated aligned scaffolds showed physiological-like morphology, while the overall superior behavior corresponded to the YIGSR-PLGA.…”

Section: Co-electrospinning With Biological Materialsmentioning

confidence: 99%

“…Fibronectin is the most widely used biological substance for cardiac cells and has been coated on chitosan [68], PCL [153] [84], PLA [154] and PU [99]. Collagen type-I was also used on P(L-D,L)LA [111] and laminin on PLGA [121]. In all cases, the cells show improved attachment, proliferation, and viability, while in some cases the desired shape of CMs is achieved.…”

Section: Co-electrospinning With Conductive Materialsmentioning

confidence: 99%

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Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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“…The use of electrospun fibres for the support of cardiac cells has an advantage due to similarity to extracellular matrix morphology, as well as the ability to tailor fibres in dimension, composition, and functionality. Different types of fibre materials have been used for stem cell graft materials, such as nano-and micro-sized fibres with different polymer compositions [5] and bio-functionalised fibres [6]. The 3-dimensional morphology provided by the fibres makes for a good basis for a cardiac patch.…”

Section: Introductionmentioning

confidence: 99%

Electroactive 3D materials for cardiac tissue engineering

et al. 2015

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Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Cited by 99 publications

References 43 publications

Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

Electroactive 3D materials for cardiac tissue engineering

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