Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Due to their good mechanical stability compared to gelatin, collagen or polyethylene glycol nanofibers and slow degradation rate, biodegradable poly-ε-caprolactone (PCL) nanofibers are promising material as scaffolds for bone and soft-tissue engineering. Here, PCL nanofibers were prepared by the electrospinning method and then subjected to surface functionalization aimed at improving their biocompatibility and bioactivity. For surface modification, two approaches were used: (i) COOH-containing polymer was deposited on the PCL surface using atmospheric pressure plasma copolymerization of CO 2 and C 2 H 4 , and (ii) PCL nanofibers were coated with multifunctional bioactive nanostructured TiCaPCON film by magnetron sputtering of TiC-CaO-Ti 3 PO x target. To evaluate bone regeneration ability in vitro, the surface-modified PCL nanofibers were immersed in simulated body fluid (SBF, 1×) for 21 days. The results obtained indicate different osteoblastic and epithelial cell response depending on the modification method. The TiCaPCON-coated PCL nanofibers exhibited enhanced adhesion and proliferation of MC3T3-E1 cells, promoted the formation of Ca-based mineralized layer in SBF and, therefore, can be considered as promising material for bone tissue regeneration. The PCL-COOH nanofibers demonstrated improved adhesion and proliferation of IAR-2 cells, which shows their high potential for skin reparation and wound dressing.

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“…No significant differences for cell adhesion and spreading [39] PLA/TSF, PLA/TSF/HA 1.5× SBF doped with 1 wt% of asparaginic acid…”

Section: Mg-63mentioning

confidence: 94%

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Permyakova

Kiryukhantsev-Korneev

Gudz

et al. 2019

Nanomaterials

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“…However, to date, ACP is rarely used as a graft for bone regeneration mainly because of its rapid dissolution and release of inorganic ion, leading to the diminished mechanical properties 17 . Hybridizing ACP with polymer matrix is a promising choice to overcome these defects 30 31 . Here, we develop ACP particle and poly(L-lactic acid) (PLLA) based nanofibrous scaffold by electrospinning method, aiming to achieve the long-term and adjustable delivery of calcium and orthophosphate ions.…”

mentioning

confidence: 99%

Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold

Niu

Liu

Tian

et al. 2017

Sci Rep

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The purpose of this study is to investigate electrospinning poly(L-lactic acid) (PLLA) nanofibrous scaffold with different contents of amorphous calcium phosphate (ACP), which is suitable for using in bone regeneration through sustained release of calcium and orthophosphate ions. Three groups of nanofibrous scaffolds, ACP-free PLLA, ACP-5 wt%/PLLA and ACP-10 wt%/PLLA, are developed and characterized by scanning electron microscopy and gel permeation chromatography. Calcium and phosphate colorimetric assay kits are used to test ions released from scaffold during hydrolytic degradation. The results show ACP-5 wt%/PLLA and ACP-10 wt%/PLLA scaffolds have relatively high degradation rates than ACP-free PLLA group. The bioactivity evaluation further reveals that ACP-5 wt%/PLLA scaffold presents more biocompatible feature with pre-osteoblast cells and significant osteogenesis ability of calvarial bone defect. Due to the facile preparation method, sustained calcium and orthophosphate release behavior, and excellent osteogenesis capacity, the presented ACP/PLLA nanofibrous scaffold has potential applications in bone tissue engineering.

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“…An RYJ-6A diffusion test apparatus (Shanghai Huang-Hai Drug Control Instrument Co., Ltd., Shanghai, China) was exploited to conduct the ex vivo permeation experiments. The diffusion area of each cell was 2.60 cm 2 . Each donor and receptor compartment was filled with 1 and 7.2 mL of PBS, respectively.…”

Section: Ex Vivo Permeation Testsmentioning

confidence: 99%

“…After half a century of pharmaceutical development, research on drug delivery is gradually extricating itself from the traditional models of drug controlled release, in which drug-release profiles often rely solely on the chemical and physical properties of pharmaceutical excipients. [1][2][3] New combined models are frequently developed, in which complicated drug controlled-release profiles (such as multiple-phase release, dual release, controlled release of multiple active ingredients in one dosage form, simultaneous control release of time and initial place, or specific release of place and release rate) are provided for improved therapeutic effects. [4][5][6] Among them, colon-specific (or colon-targeted) pulsatile drug delivery, which allows a time-and site-specific release of drugs in the colon after a predetermined lag time, is a new advanced drug controlled-release model.…”

Section: Introductionmentioning

confidence: 99%

Colon-specific pulsatile drug release provided by electrospun shellac nanocoating on hydrophilic amorphous composites

Yang¹,

Liu²,

Yu³

et al. 2018

IJN

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BackgroundColon-specific pulsatile drug release, as a combined drug controlled-release model, is a useful drug delivery manner for a series of diseases. New nanomedicines and related preparation methods are highly desired.MethodsWith diclofenac sodium (DS) as a model drug, a new type of structural nanocomposite (SC), in which composite polyvinylpyrrolidone (PVP)–DS core was coated by shellac, was fabricated via modified coaxial electrospinning. For comparison, traditional PVP–DS monolithic hydrophilic nanocomposites (HCs) were generated using a traditional blending process. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), water contact angle (WCA), and in vitro dissolution and ex vivo permeation tests were conducted to characterize the composites.ResultsSEM images demonstrated that both composites were linear nanofibers with smooth surface morphology and cross sections. TEM disclosed that the SCs had a thin shellac sheath layer of approximately 12 nm. XRD and ATR-FTIR results demonstrated that the crystalline DS was converted into amorphous composites with PVP because of favorable secondary interactions. WCA and in vitro dissolution tests demonstrated that the sheath shellac layers in SC could resist acid conditions and provide typical colon-specific pulsatile release, rather than a pulsatile release of HC under acid conditions. Ex vivo permeation results demonstrated that the SCs were able to furnish a tenfold drug permeation rate than the DS particles on the colon membrane.ConclusionA new SC with a shellac coating on hydrophilic amorphous nanocomposites could furnish a colon-specific pulsatile drug release profile. The modified coaxial process can be exploited as a useful tool to create nanocoatings.

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Electrospinning of calcium phosphate-poly(D,L-lactic acid) nanofibers for sustained release of water-soluble drug and fast mineralization

Cited by 25 publications

References 41 publications

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold

Colon-specific pulsatile drug release provided by electrospun shellac nanocoating on hydrophilic amorphous composites

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