Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold

Abstract

The purpose of this study is to investigate electrospinning poly(L-lactic acid) (PLLA) nanofibrous scaffold with different contents of amorphous calcium phosphate (ACP), which is suitable for using in bone regeneration through sustained release of calcium and orthophosphate ions. Three groups of nanofibrous scaffolds, ACP-free PLLA, ACP-5 wt%/PLLA and ACP-10 wt%/PLLA, are developed and characterized by scanning electron microscopy and gel permeation chromatography. Calcium and phosphate colorimetric assay kits are used to test ions released from scaffold during hydrolytic degradation. The results show ACP-5 wt%/PLLA and ACP-10 wt%/PLLA scaffolds have relatively high degradation rates than ACP-free PLLA group. The bioactivity evaluation further reveals that ACP-5 wt%/PLLA scaffold presents more biocompatible feature with pre-osteoblast cells and significant osteogenesis ability of calvarial bone defect. Due to the facile preparation method, sustained calcium and orthophosphate release behavior, and excellent osteogenesis capacity, the presented ACP/PLLA nanofibrous scaffold has potential applications in bone tissue engineering.

Nucleation of Brushite and Hydroxyapatite from Amorphous Calcium Phosphate Phases Revealed by Dynamic In Situ Raman Spectroscopy

“…Phosphorus (P) and calcium (Ca) are also essential elements for the development of living organisms. Moreover, calcium phosphates find wide medical and industrial applications in dentistry, orthopedics, reconstructive surgery, food additives, fertilizers, and water depollution (1)(2)(3)(4)(5)(6). The formation of calcium phosphates and the interactions and associations of these minerals with organic macromolecules have been widely investigated under in vivo and in vitro conditions in the last decades (7-10) in order to identify the nature of first primary precipitating particles, their lifetime and their chemical transformation into other minerals in aqueous media (11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”

Biological evaluation of the modified nano-amorphous phosphate calcium doped with citrate/poly-amino acid composite as a potential candidate for bone repair and reconstruction

“…The study investigated a novel n-ACP-cit/PAA composite combining the good degradability, superior bioactivity, and osteogenic activity of n-ACP-cit powder with the excellent properties of PAA matrix that is similar to the polypeptide architecture of natural collagen proteins [ 24 ]. Among the factors influencing the physicochemical and biological properties of the composites, the interface combination between the different components of the composites was significantly important [ 25 ].…”

Biomimetic delivery of signals for bone tissue engineering

“…200 , 201 Of note, biomechanical stimulation can also affect the degradation and release properties of PLA and PLGA polymers. 202 – 204 …”