Electrospun PLGA/collagen nanofibrous membrane as early-stage wound dressing

Liu, Shih‐Jung; Kau, Yi-Chuan; Chou, C.-T.; Chen, Jan‐Kan; Wu, Ren‐Chin; Yeh, Wen‐Ling

doi:10.1016/j.memsci.2010.03.012

Cited by 224 publications

(96 citation statements)

References 23 publications

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“…Electrospun nanofibers were in vivo extracellular matrix (ECM) like which are promising for further applications in tissue engineering and regenerative medicine [10,11].…”

Section: Introductionmentioning

confidence: 99%

Patch method for culture of primary hippocampal neurons

Tang

Severino

Iseppon

et al. 2017

Microelectronic Engineering

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In vitro culture of primary neurons, especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method to culture primary neurons on a monolayer of gelatin nanofibers, electrospun and crosslinked on a microfabricated honeycomb frame of poly (ethylene glycol) diacrylate (PEGDA). By using such a patch method, neural networks could be formed with a minimal cell-exogenous materials contact and a maximal exposure of the cells to the medium. Interestingly, hippocampal cells, especially astrocytes, showed in-vivo like morphology and most of neurons were found in the porous areas inside the honeycomb compartments although the nanofibers were deposited everywhere of the frame. Finally, calcium imaging showed that primary neurons have a higher degree of neural activity on the patch than on glass.

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“…Electrospun nanofibers were in vivo extracellular matrix (ECM) like which are promising for further applications in tissue engineering and regenerative medicine [10,11].…”

Section: Introductionmentioning

confidence: 99%

Patch method for culture of primary hippocampal neurons

Tang

Severino

Iseppon

et al. 2017

Microelectronic Engineering

View full text Add to dashboard Cite

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“…A variety of polymer-based nanofibers have been applied as wound dressings, including polyvinylalcohol [143], chitosan [130], gelatine [144], hyaluronic acid [145], collagen and collagen-based composites [146,147], polyethersulfone [148], and polyurethane/polyurethane nanocomposites [149,150]. Polyethersulfone (PES), a synthetic and biocompatible polymer, was electrospun into nanofibers and applied as an epidermal wound dressing, which proved to be effective in improving the skin healing [148].…”

Section: Polymer-based Nanofibers For Wound Dressingmentioning

confidence: 99%

Multifunctional Nanofibers towards Active Biomedical Therapeutics

et al. 2015

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One-dimensional (1-D) nanostructures have attracted enormous research interest due to their unique physicochemical properties and wide application potential. These 1-D nanofibers are being increasingly applied to biomedical fields owing to their high surface area-to-volume ratio, high porosity, and the ease of tuning their structures, functionalities, and properties. Many biomedical nanofiber reviews have focused on tissue engineering and drug delivery applications but have very rarely discussed their use as wound dressings. However, nanofibers have enormous potential as wound dressings and other clinical applications that could have wide impacts on the treatment of wounds. Herein, the authors review the main fabrication methods of nanofibers as well as requirements, strategies, and recent applications of nanofibers, and provide perspectives of the challenges and opportunities that face multifunctional nanofibers for active therapeutic applications.

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“…Typically, these wounds require extensive hospitalization, labour intensive clinical procedures and costly wound care products, representing a major burden over total world healthcare expenditure [9,153,175]. Several skin therapies and wound care products have been developed and tested through pre-clinical and randomized controlled clinical trials with the ultimate goal of promoting the repair and regeneration of functional skin [44,49,84,86,88,103,124,189,195,212]. Traditional treatments are based on the use of grafts (which includes auto-, allo-and xenograft varieties), herbal and animalderived compounds, silver-containing and traditional dressings.…”

Section: Introductionmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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Electrospun PLGA/collagen nanofibrous membrane as early-stage wound dressing

Cited by 224 publications

References 23 publications

Patch method for culture of primary hippocampal neurons

Patch method for culture of primary hippocampal neurons

Multifunctional Nanofibers towards Active Biomedical Therapeutics

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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