Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferation

Bai, Lingchuang; Li, Qian; Duo, Xinghong; Hao, Xiao‐Jiang; Zhang, Wencheng; Shi, Changcan; Guo, Jintang; Ren, Xiang‐Kui; Feng, Yakai

doi:10.1039/c7ra06253b

Cited by 31 publications

(31 citation statements)

References 57 publications

(63 reference statements)

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“…PCL‐PIBMD has been electrospun with silk fibroin (SF) to prepare PCL‐PIBMD/SF (denoted as PS) nanofiber film in our previous study . PCL‐PIBMD provides it with high mechanical strength and SF with cell affinity sites . This PS nanofiber film was used to load gene complexes and could promote the proliferation of ECs in vitro .…”

Section: Introductionmentioning

confidence: 99%

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Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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Artificial small‐caliber vascular grafts are still limited in clinical application because of thrombosis, restenosis, and occlusion. Herein, a small‐caliber vascular graft (diameter 2 mm) is fabricated from poly(ε‐caprolactone)‐b‐poly(isobutyl‐morpholine‐2,5‐dione) (PCL‐PIBMD) and silk fibroin (SF) by electrospinning technology and then biofunctionalized with low‐fouling poly(ethylene glycol) (PEG) and two cell‐adhesive peptide sequences (CREDVW and CAGW) with the purpose of enhancing antithrombogenic activity and endothelialization. The successful grafting of PEG and peptide sequences is confirmed by X‐ray photoelectron spectroscopy. The suitable surface wettability of the modified vascular graft is testified by water contact angle analysis. The surface hemocompatibility is verified by platelet adhesion assays and protein adsorption assays, and the results demonstrate that both platelet adhesion and protein adsorption on the biofunctionalized surface are significantly reduced. In vitro studies demonstrate that the biofunctionalized surface with suitable hydrophilicity and cell‐adhesive peptides can selectively promote the adhesion, spreading, and proliferation of human umbilical vein endothelial cells. More importantly, compared with control groups, this biofunctionalized small‐caliber vascular graft shows high long‐term patency and endothelialization after 10 weeks of implantation. The biofunctionalization with PEG and two cell‐adhesive peptide sequences is an effective method to improve the endothelialization and long‐term performance of synthetic vascular grafts.

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Section: Introductionmentioning

confidence: 99%

“…PCL‐PIBMD provides it with high mechanical strength and SF with cell affinity sites . This PS nanofiber film was used to load gene complexes and could promote the proliferation of ECs in vitro . PS surface is relatively hydrophobic.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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“…Polyethylenimine (branched PEI, Mw = 10 kDa), methoxypoly(ethylene glycol) (mPEG, Mw = 5 kDa), sorbitol, stannous octoate (Sn (Oct) 2 ) were purchased from Sigma‐Aldrich (St. Louis, USA). N‐Hydroxy succinimide (NHS), 1‐ethyl‐3‐(3‐(dimethylamino)propyl)‐carbodiimide hydrochloride (EDC), succinic anhydride, diallylcarbamyl chloride, and 2,2‐dimethoxy‐2‐phenylacetophenone (DMPA) were obtained from Tianjin Heowns Biochemical Technology Co., Ltd. CAGW (Cys‐Ala‐Gly‐Trp) and TAT‐NLS (Gly‐Arg‐Lys‐Lys‐Arg‐Arg‐Gln‐Arg‐Arg‐Arg‐Pro‐Lys‐Lys‐Lys‐Arg‐Lys‐Val) peptides were purchased from GL Biochem (Shanghai) Ltd. EA.hy926 cells (human umbilical vein endothelial cells) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and cultured as described before . Other chemicals and biological reagents were supplied by the companies referred in our previous study …”

Section: Methodsmentioning

confidence: 99%

“…However, small‐caliber artificial vascular grafts usually encounter some problems such as thrombosis and restenosis in clinic application . Gene therapy offers a promising way to solve these problems by means of promoting the proliferation, migration, and differentiation of endothelial cells (ECs) on graft surface . Generally, gene therapy needs gene carriers to overcome multiple barriers in gene delivery and finally deliver therapeutic genes into target cells .…”

Section: Introductionmentioning

confidence: 99%

“…2,3 Gene therapy offers a promising way to solve these problems by means of promoting the proliferation, migration, and differentiation of endothelial cells (ECs) on graft surface. 4,5 Generally, gene therapy needs gene carriers to overcome multiple barriers in gene delivery and finally deliver therapeutic genes into target cells. [6][7][8][9][10] Therefore, it is of significance to develop a safe and efficient gene carrier to realize gene therapy.…”

Section: Introductionmentioning

confidence: 99%

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CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

Duo

Bai

Wang

et al. 2019

Polymers for Advanced Techs

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Gene therapy has attracted much attention in vascular tissue engineering. However, it is still challenging to develop a novel gene carrier with multifunction to overcome the barriers in gene delivery. Herein, the multitargeting gene complexes were developed based on methoxy‐poly(ethylene glycol)‐b‐poly‐(D,L‐lactide‐co‐glycolide) (mPEG‐b‐PLGA), poly(d,l‐lactide‐co‐glycolide)‐g‐polyethylenimine‐g‐CAGW (PLGA‐g‐PEI‐g‐CAGW), cell‐penetrating peptide YGRKKRRQRRR (TAT), nuclear localization signals (NLS), and pEGFP‐ZNF580 (pDNA) with the purpose of enhancing the transfection of endothelial cells (ECs). The low cytotoxic multitargeting gene complexes could be easily prepared by adjusting the weight ratio of mPEG‐b‐PLGA and PLGA‐g‐PEI‐g‐CAGW. Meanwhile, CAGW peptide with selectively ECs‐targeting ability and TAT‐NLS peptide sequence with both cell‐penetrating ability and nuclear targeting capacity were simultaneously introduced into gene complexes in order to enable them with the multitargeting function so as to improve their gene delivery capacity. The pDNA loading capacity of these gene complexes was confirmed by agarose gel electrophoresis assay. MTT results demonstrated that the relatively cell viability of the multitargeting gene complexes was higher than those of other groups. These multitargeting gene complexes showed higher internalization and transfection efficiencies than other groups. These results revealed that CAGW and TAT‐NLS peptide sequences benefited for efficient gene delivery. Furthermore, the wound healing assay demonstrated that the multitargeting gene complexes could promote the proliferation and migration of ECs. These results collectively demonstrated that CAGW and TAT‐NLS peptides functionalized gene delivery system could effectively enhance the transfection of ECs, which has great potential in vascular tissue engineering.

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Biofabrication of an Esophageal Tissue Construct from a Polymer Blend Using 3D Extrusion‐Based Printing

et al. 2022

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Many tissue engineering approaches are being explored to offer solutions for diseased esophageal tissue and its repair. However, classical techniques in tissue engineering are not capable of recapitulating the structural and functional parameters of native esophagi. Esophageal 3D bioprinting is yet an emerging field but holds great promise in meeting this challenge. Herein, the use of extrusion‐based 3D printing is examined to generate esophageal substitutes from a polymer blend‐based formulation. The fabricated 3D esophageal structures are printed at a very high speed without collapse and without the use of supporting material. In vitro analysis reveals that the printed material enables cell adhesion, proliferation, and migration into the deeper zones. Moreover, the designed construct is suturable to the native esophagus and exhibits no leakage while offering mechanical properties similar to that of the native tissue. Overall, these biochemical and biomechanical features make the reported artificial esophagus a promising solution for the repair of damaged esophagi.

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Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferation

Abstract: PCL-PIBMD/SF scaffolds can maintain the integrity of plasmid complexes loaded in scaffolds, and thereby enhance the proliferation of endothelial cells.

Cited by 31 publications

References 57 publications

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

Biofabrication of an Esophageal Tissue Construct from a Polymer Blend Using 3D Extrusion‐Based Printing

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