Electrospun multilayer chitosan scaffolds as potential wound dressings for skin lesions

Trinca, Rafael Bergamo; Westin, Cecília Buzatto; Silva, José Alberto Fracassi da; Moraes, Ângela Maria

doi:10.1016/j.eurpolymj.2017.01.021

Cited by 120 publications

(75 citation statements)

References 42 publications

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“…By contrast, contact angles of more than 90° correspond to low wettability. 29 As shown in Figure 4A the contact angle value of the PNNM scaffold is lower than that of other scaffolds. In Figure 4B, the swelling ratios of all of the scaffolds are not significantly different.…”

Section: Morphological Characteristics and Physical Properties Of Msnmentioning

confidence: 83%

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

Zhu¹,

Song²,

Ju³

et al. 2019

IJN

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PurposeIn this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methodsWe first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).ResultsNAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.ConclusionA PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering.

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Section: Morphological Characteristics and Physical Properties Of Msnmentioning

confidence: 83%

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

Zhu¹,

Song²,

Ju³

et al. 2019

IJN

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“…2 However, it is a challenge to yield suitable poly(ε-caprolactone)-based biomaterials for tissue engineering purposes because poly(ε-caprolactone) has high hydrophobicity and low water adsorption. 3 Therefore, poly(ε-caprolactone) should be blended to polysaccharides (chitosan, 2 cellulose acetate, 4 and starch 5 ) or associated to materials with biological properties such as dexamethasone (notable for its anti-inflammatory properties) 6,7 to produce electrospun membranes for applications in tissue engineering field. These materials can enhance the cytocompatibility, promoting better anchorage of cells onto poly(ε-caprolactone)-based membrane surfaces.…”

Section: Introductionmentioning

confidence: 99%

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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Poly(ε-caprolactone), an aliphatic polyester with biodegradability and cytocompatibility, has been used to create scaffolds for tissue engineering purposes. However, the hydrophobicity and low water absorptivity of poly(ε-caprolactone) reduce cell anchorage on their membranes. Here, poly(ε-caprolactone)-based scaffolds were prepared by electrospinning of poly(ε-caprolactone)chitosan blend, and poly(ε-caprolactone)-dexamethasone solution. Chitosan and dexamethasone play an essential role to increase the scaffolding performance of poly(ε-caprolactone)-based electrospun membranes. A poly(ε-caprolactone) membrane without chitosan and dexamethasone did not provide satisfactory results to promote cell culture of adipose mesenchymal stem cells (AMSCs). Compared to the poly(ε-caprolactone)-dexamethasone surface, poly(ε-caprolactone)chitosan membrane imparts better cytoskeletal reorganization, and cell spreading, increasing the strength of cell attachment. Also, poly(ε-caprolactone)-chitosan composite provides strong antimicrobial activity against Pseudomonas aeruginosa (ca. 90% inhibition). Therefore, the poly(ε-caprolactone)-chitosan composite is a better alternative to treat skin diseases and promote skin regeneration than conventional approaches based on dexamethasone.

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“…Blends of PEG-PCL polymers have been used as a strategy to modify the mechanical properties, wettability, biocompatibility, and solubility of these scaffolds in water and thus accelerate the degradability of the scaffolds [29,30]. The mechanical properties of scaffolds designed for the treatment of skin lesions should match those of the skin itself [31]. For native skin, values of Young modulus are reported to be within the range of ~5-30 MPa [32].…”

Section: Discussionmentioning

confidence: 99%

Development of bioactive electrospun scaffolds suitable to support skin fibroblasts and release Lucilia sericata maggot excretion/secretion

Giacaman

Styliari

Taresco

et al. 2019

Preprint

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Larval therapy has been reported to exert beneficial actions upon chronic wound healing by promoting granulation tissue formation, antimicrobial activity and degrading necrotic tissue. However, the use of live maggots is problematic for patient acceptance, and thus there is a need to develop materials which can adsorb and release therapeutic biomolecules from maggot secretions. Here we describe the fabrication of a novel bioactive scaffold that can be loaded with Lucilia sericata maggot excretion/secretion (L. sericata maggot E/S) for wound therapy, and which also provides structural stability for mammalian cell-growth and migration. We show that electrospun scaffolds can be prepared from polycaprolactone-poly (ethylene glycol)-block copolymer (PCL-b-PEG) blended with PCL, to form fibres with average diameters of ~4 μm. We further demonstrate that the fibres are able to be loaded with L. sericata maggot E/S, in order to influence fibroblast migration through protease activity. Finally, we show that after 21 days, the cumulative amount of released L. sericata maggot E/S was ~14 μg/mL from PCLb-PEG/PCL scaffolds and that the protease activity of L. sericata maggot E/S was preserved when PCL-b-PEG/PCL scaffolds were used as the release platform.

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Electrospun multilayer chitosan scaffolds as potential wound dressings for skin lesions

Cited by 120 publications

References 42 publications

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Development of bioactive electrospun scaffolds suitable to support skin fibroblasts and release Lucilia sericata maggot excretion/secretion

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