Electrospun Hyaluronan‐Gelatin Nanofibrous Matrix for Nerve Tissue Engineering

Liou, Hau-Min; Rau, Lih-Rou; Huang, Chun-Chiang; Lu, Meng-Ru; Hsu, Fu‐Yin

doi:10.1155/2013/613638

Cited by 20 publications

(8 citation statements)

References 34 publications

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“…There was a significant difference in the expression of Islet-1 and ChAT between 2D and 3D cultures at first and second week of induction with PCL (30:70) increased the number of differentiated cells toward neural lineages in comparison with PCL or gelatin alone [26]. According to Liou et al, gelatin/ hyaluronan electrospun scaffold upregulates the expression of neural genes in neural cells of the peripheral system in comparison with gelatin membrane [44].…”

Section: Discussionmentioning

confidence: 96%

Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems

Faghihi

Mirzaei

et al. 2015

Mol Neurobiol

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Many people worldwide suffer from motor neuron-related disorders such as amyotrophic lateral sclerosis and spinal cord injuries. Recently, several attempts have been made to recruit stem cells to modulate disease progression in ALS and also regenerate spinal cord injuries. Chorion-derived mesenchymal stem cells (C-MSCs), used to be discarded as postpartum medically waste product, currently represent a class of cells with self renewal property and immunomodulatory capacity. These cells are able to differentiate into mesodermal and nonmesodermal lineages such as neural cells. On the other hand, gelatin, as a simply denatured collagen, is a suitable substrate for cell adhesion and differentiation. It has been shown that electrospinning of scaffolds into fibrous structure better resembles the physiological microenvironment in comparison with two-dimensional (2D) culture system. Since there is no report on potential of human chorion-derived MSCs to differentiate into motor neuron cells in two- and three-dimensional (3D) culture systems, we set out to determine the effect of retinoic acid (RA) and sonic hedgehog (Shh) on differentiation of human C-MSCs into motor neuron-like cells cultured on tissue culture plates (2D) and electrospun nanofibrous gelatin scaffold (3D).

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Section: Discussionmentioning

confidence: 96%

Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems

Faghihi

Mirzaei

et al. 2015

Mol Neurobiol

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“…In natural tissues, cells are surrounded by extracellular matrix (ECM), which is a complex composed of nanosized proteins and glycosaminoglycans (GAGs) [1][2][3]. Electrospinning has been proven to be a relatively simple technique that utilizes high-voltage electrostatic field to drive the polymer solutions or melts to produce nanofibers with diameter in the range from micrometers down to tens of nanometers, which has been investigated as a polymer processing technique for tissue engineering application in recent years [4,5]. Therefore, scaffolds fabricated by electrospinning could mimic the architecture of ECM.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Electrospun Polycaprolactone Blended with Chitosan‐Gelatin Complex Nanofibrous Mats

Qian

Zhang

Zheng

et al. 2014

Journal of Nanomaterials

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Design and fabrication of nanofibrous scaffolds should mimic the native extracellular matrix. This study is aimed at investigating electrospinning of polycaprolactone (PCL) blended with chitosan-gelatin complex. The morphologies were observed from scanning electron microscope. As-spun blended mats had thinner fibers than pure PCL. X-ray diffraction was used to analyze the degree of crystallinity. The intensity at two peaks at 2θof 21° and 23.5° gradually decreased with the percentage of chitosan-gelatin complex increasing. Moreover, incorporation of the complex could obviously improve the hydrophilicity of as-spun blended mats. Mechanical properties of as-spun nanofibrous mats were also tested. The elongation at break of fibrous mats increased with the PCL content increasing and the ultimate tensile strength varied with different weight ratios. The as-spun mats had higher tensile strength when the weight ratio of PCL to CS-Gel was 75/25 compared to pure PCL. Both as-spun PCL scaffolds and PCL/CS-Gel scaffolds supported the proliferation of porcine iliac endothelial cells, and PCL/CS-Gel had better cell viability than pure PCL. Therefore, electrospun PCL/Chitosan-gelatin nanofibrous mats with weight ratio of 75/25 have better hydrophilicity mechanical properties, and cell proliferation and thus would be a promising candidate for tissue engineering scaffolds.

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“…This protein was electrospun easily and was crosslinked with glutaraldehyde to improve water‐resistant ability 35,95 . Natural polymers were combined and with other biological materials such as the brain extracellular matrix to up‐regulate the expression of neural genes, enhance the neurite extension, and improve the neural outgrowth and regeneration 57,96‐98 . Melanin, as an electroactive, antioxidant, and bioactive compound was blended with silk‐fibroin to electrospun nanofibrous nerve scaffolds.…”

Section: Utilized Polymers For the Preparation Of The Nerve Scaffoldsmentioning

confidence: 99%

Regeneration of the peripheral nerve via multifunctional electrospun scaffolds

Ghane

Khalili

Khorasani

et al. 2020

J Biomedical Materials Res

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Over the last two decades, electrospun scaffolds have proved to be advantageous in the field of nerve tissue regeneration by connecting the cavity among the proximal and distal nerve stumps growth cones and leading to functional recovery after injury. Multifunctional nanofibrous structure of these scaffolds provides enormous potential by combining the advantages of nano‐scale topography, and biological science. In these structures, selecting the appropriate materials, designing an optimized structure, modifying the surface to enhance biological functions and neurotrophic factors loading, and native cell‐like stem cells should be considered as the essential factors. In this systematic review paper, the fabrication methods for the preparation of aligned nanofibrous scaffolds in yarn or conduit architecture are reviewed. Subsequently, the utilized polymeric materials, including natural, synthetic and blend are presented. Finally, their surface modification techniques, as well as, the recent advances and outcomes of the scaffolds, both in vitro and in vivo, are reviewed and discussed.

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Electrospun Hyaluronan‐Gelatin Nanofibrous Matrix for Nerve Tissue Engineering

Cited by 20 publications

References 34 publications

Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems

Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems

Fabrication and Characterization of Electrospun Polycaprolactone Blended with Chitosan‐Gelatin Complex Nanofibrous Mats

Regeneration of the peripheral nerve via multifunctional electrospun scaffolds

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