Electrospun gelatin/polyurethane blended nanofibers for wound healing

Abstract

In this study, we prepared a blended nanofiber scaffold using synthetic and natural polymers, polyurethane (PU) and gelatin respectively, using the electrospinning method to prepare a material for wound dressing. In order to confirm the properties of this gelatin/PU blended nanofiber scaffold, we performed scanning electron microscopy, atomic force microscopy, attenuated total reflectance Fourier-transform infrared spectroscopy, thermal gravimetric analysis, contact angle, water uptake, mechanical property, recovery, and degradation tests, and cellular response. The results obtained indicate that the mean diameter of these nanofibers was uniformly electrospun and ranged from 0.4 to 2.1 microm. According to the results, when the amount of gelatin in the blended solution decreased, the contact angle increased and water uptake of the scaffold decreased concurrently. In the mechanical tests, the blended nanofibrous scaffolds were elastic, and elasticity increased as the total amount of PU increased. Moreover, as the total amount of gelatin increased, the cell proliferation increased with the same amount of culture time. Therefore, this gelatin/PU blended nanofiber scaffold has potential application for use as a wound dressing.

The Use of Natural Polymers in Tissue Engineering: A Focus on Electrospun Extracellular Matrix Analogues

“…Electrospun gelatin scaffolds have been used for various applications such as wound healing [59,60], nerve [52,61,62], dental [49], bone [48,50,57,63], dermal tissue engineering applications [64,65] and vascular grafts [66]. Much of the work published on electrospun gelatin scaffolds has been directed towards its use in cardiac tissue engineering applications.…”

Wound Healing and Electrospun Wound Dressings: Review

“…62 Bir diğer ça-lışmada ise poliüretan-jelatin karışımı ile hazırlanan nanoliflerin karakterizasyonları yapıla-rak yara tedavisinde potansiyel olarak kullanılabi-leceği belirtilmiştir. 63 Kitozan, non toksik, biyoparçalanabilir, biyouyumlu olması, antibakteriyel, antimikotik özel-lik göstermesi ve hemostatik aktivitesi nedeni ile elektro-çekim yöntemiyle hazırlanan nanolif yapı-sındaki yara örtülerinin hazırlanmasında en çok kullanılan polimerlerden biridir. [64][65][66][67][68][69][70][71] Kitozanın, polivinil alkol, polikaprolakton, poli (L-laktit), poli(Llaktit)/poli(etilen glikol), polietilenoksit, kollajen kombinasyonları ile hazırlanan nanolif yapısındaki yara örtüleriyle başarılı sonuçlar alınmıştır.…”

Electrospun Polyurethane Nanofibers

“…69937 19 wound dressing materials, sensors, in biomedical applications, drug delivery, etc. [21,[37][38][39][40][41][42][43][44].…”

“…It has reported that semipermeable dressings, many of which are PU, enhance wound healing [21,39]. Degradable and biocompatible aliphatic PU could also be formed into scaffolds via melt electrospinning [42].…”